Discontinuities in the DNA synthesized in an Excision-defective strain of Escherichia coli following ultraviolet irradiation

Minding the gap: The underground functions of BRCA1 and BRCA2 at stalled replication forks

“…Single mutants of recA or uvrA are mildly sensitive to UV light, but the recA uvrA double mutant is absolutely intolerant of UV damage; RecA is essential for survival in the presence of UV lesions that cannot be excised. Clues regarding the role of RecA in this process came from physical analysis of newly synthesized daughter strands in UV exposed uvrA mutants [30]. UV light caused a reduction in the average size of newly synthesized daughter strands, but during recovery from UV damage, daughter strands reverted to normal length.…”

A general approach for purifying proteins encoded by cloned genes without using a functional assay: isolation of the uvrA gene product from radiolabeled maxicells

“…2 1 of logarithmic phase DM1415 carrying either pUkR2000 or pDR1996 grown at 37%C in K-medium (25) supplemented with 5 pg/ml tetracycline and ZO0O ug/ml ampicillin were irradiated with a fluence of 3b-50 J/m2 from a germicidal UV lamp. After irradiation, cycloserine (200O ig/ml), carbenicillin (100 ug/ml) and methicillin (100( pg/ml) were added and the incubation was continued with shaking at 37uC for 12-14 hrs.…”

DNA repair, DNA replication and human disorders: A personal journey

“…Dean Rupp and Paul Howard-Flanders had published their seminal work on postreplication repair in E. coli a couple of years earlier, in which they demonstrated daughter-strand gaps in UV-irradiated cells and showed that these gaps were subsequently sealed [10]. I thought it would be interesting to look if something similar happened in mammalian cells.…”