Discoidin domain receptor 1 mediates collagen-induced nitric oxide production in J774A.1 murine macrophages

Kim, Sang Hyun; Lee, So‐Young; Suk, Kyoungho; Bark, Hyun; Jun, Chang Duk; Kim, Young Ho; Choi, Choong Hyeok; Yoshimura, Teizo

doi:10.1016/j.freeradbiomed.2006.10.052

Cited by 60 publications

(39 citation statements)

References 29 publications

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“…Electophoretic mobility shift assay (EMSA) was performed as previously described [11]. In brief, nuclear extract was prepared and incubated for 20 min at room temperature with 20 µg of bovine serum albumin, 2 µg of poly (dI-dC) (Pharmacia, Uppsala, Sweden), 2 µl of buffer C (20 mM Hepes/KOH, 20% glycerol, 100 mM KCl, and 0.5 mM PMSF, pH 7.9), 4 µl of buffer F (20% Ficoll-400, 100 mM Hepes/KOH, 300 mM KCl, 10 mM DTT, and 0.5 mM PMSF, pH 7.9), and 20,000 cpm of 32 P-labeled probe encoding the consensus sequence for NF-κB-binding sites (5′-CAGAGGGGACTTTCCGA GAG-3′) in a final volume of 20 µl.…”

Section: Electophoretic Mobility Shift Assaymentioning

confidence: 99%

DDR2 inhibition reduces migration and invasion of murine metastatic melanoma cells by suppressing MMP2/9 expression through ERK/NF-κB pathway

Poudel¹,

Lee²,

Kim³

2015

ABBS

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Metastatic melanoma is one of the most deadly and evasive cancers. Collagen I in the extracellular matrix promotes the migration and invasion of tumor cells through the production of matrix metalloproteinase (MMP) 2 and 9. Discoidin domain receptor (DDR) 2 is a collagen receptor that is implicated in several cancer types including breast and prostate cancers. However, the role of DDR2 in the migration and invasion of murine melanoma cells is less studied. In the present study, we investigated the effects and underlying mechanisms of DDR2 in migration and invasion of B16BL6 melanoma cells in response to collagen I. Results demonstrated that DDR2 is expressed and is phosphorylated by collagen I in the cells. Upon down-regulation of DDR2 using small-interfering RNA (siRNA) approach, both of the cell migratory and invasive phenotypes were significantly attenuated when compared with the control cells. This effect was mediated via suppression of MMP2/9 upon DDR2 inhibition. Furthermore, inhibition of DDR2 by specific siRNA markedly reduced the activation of extracellular regulated kinase (ERK) 1 and 2 and nuclear factor of kappa B (NF-κB) in the cells when compared with the control cells. Overall, these data demonstrated that DDR2 siRNA-mediated suppression of ERK1/2 and NF-κB could down-regulate the expressions of MMP2/ 9 in response to collagen I to reduce the migratory and invasive phenotypes of the cells.

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Section: Electophoretic Mobility Shift Assaymentioning

confidence: 99%

DDR2 inhibition reduces migration and invasion of murine metastatic melanoma cells by suppressing MMP2/9 expression through ERK/NF-κB pathway

Poudel¹,

Lee²,

Kim³

2015

ABBS

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“…Total RNA was isolated from superficial cervicel, axillary of each group. The conditions for PCR were similar to ones previously described [16]. Briefly, 2 μl of cDNA (100 ng), 1 μl of sense and antisense primer solution (0.4 μM), 12.5 μl of SYBR Premix Ex Taq (Takarabio Inc.), and 9.5 μl of dH 2 O were mixed together to obtain a final 25 μl reaction mixture in each reaction tube.…”

Section: Real-time Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

DA-9601 suppresses 2, 4-dinitrochlorobenzene and dust mite extract-induced atopic dermatitis-like skin lesions

Choi¹,

Lee²,

Hwang³

et al. 2011

International Immunopharmacology

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“…Macrophages are important cells in the development of inflammatory responses by secreting an array of pro-inflammatory cytokines and nitric oxide (NO) in a tissue microenvironment (Gross and Wolin, 1995;Kim et al, 2007). NO plays pivotal roles in many physiological and pathophysiological processes especially in inflammatory responses as a messenger and effector molecule (Kim et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of astragalin on expression of lipopolysaccharide-induced inflammatory mediators through NF-κB in macrophages

Kim

2011

Arch. Pharm. Res.

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104

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Astragalin (kaempferol-3-O-glucoside), a newly found flavonoid from leaves of persimmon or Rosa agrestis, is known to have antiatopic dermatitis and antioxidant activity. However, the effect of astragalin on the inflammatory response is not well defined. Nitric oxide (NO) produced from the activated macrophages is well known as a mediator of inflammation. Transcription factor (NF)-κB mediates the inducible expression of a variety of genes involved in immune and inflammatory responses including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and cytokines/chemokines. In the present study, we examined the inhibitory effects of astragalin on the lipopolysaccharide (LPS)-induced inflammatory mediators. Astragalin significantly reduced LPS-induced expression of iNOS, COX-2 and cytokines/chemokines, and production of NO in J774A.1 mouse macrophages. Astragalin inhibited LPSinduced activation of NF-κB as indicated by inhibition of degradation of IκBα, nuclear translocation of NF-κB, and NF-κB dependent gene reporter assay. The inhibitory effects of astragalin on the inflammatory mediators are comparable with quercetin, a well known flavonoid possessing antioxidant and anti-inflammatory activity. Using the mouse peritoneal macrophages, we confirmed the inhibitory effect of astragalin on NO production and NF-κB activation. Taken together, our results indicate that astragalin inhibits expression of proinflammatory mediators through the inhibition of NF-κB in macrophages.

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Discoidin domain receptor 1 mediates collagen-induced nitric oxide production in J774A.1 murine macrophages

Cited by 60 publications

References 29 publications

DDR2 inhibition reduces migration and invasion of murine metastatic melanoma cells by suppressing MMP2/9 expression through ERK/NF-κB pathway

DDR2 inhibition reduces migration and invasion of murine metastatic melanoma cells by suppressing MMP2/9 expression through ERK/NF-κB pathway

DA-9601 suppresses 2, 4-dinitrochlorobenzene and dust mite extract-induced atopic dermatitis-like skin lesions

Inhibitory effect of astragalin on expression of lipopolysaccharide-induced inflammatory mediators through NF-κB in macrophages

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Discoidin domain receptor 1 mediates collagen-induced nitric oxide production in J774A.1 murine macrophages

Cited by 60 publications

References 29 publications

DDR2 inhibition reduces migration and invasion of murine metastatic melanoma cells by suppressing MMP2/9 expression through ERK/NF-&kappa;B pathway

DDR2 inhibition reduces migration and invasion of murine metastatic melanoma cells by suppressing MMP2/9 expression through ERK/NF-&kappa;B pathway

DA-9601 suppresses 2, 4-dinitrochlorobenzene and dust mite extract-induced atopic dermatitis-like skin lesions

Inhibitory effect of astragalin on expression of lipopolysaccharide-induced inflammatory mediators through NF-κB in macrophages

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DDR2 inhibition reduces migration and invasion of murine metastatic melanoma cells by suppressing MMP2/9 expression through ERK/NF-κB pathway

DDR2 inhibition reduces migration and invasion of murine metastatic melanoma cells by suppressing MMP2/9 expression through ERK/NF-κB pathway