Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis

Ye, Wei; Liu, Taomei; Zhang, Weiyang; Li, Saini; Zhu, Muzi; Li, Haohua; Kong, Yali; Xu, Luoshan

doi:10.3390/ijms20236090

Cited by 29 publications

(16 citation statements)

References 48 publications

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“…Plants usually need to respond to different environmental changes and biotic stresses. Pathogens are a well-known example of biotic stress, and B. sorokiniana is particularly important because this can cause multiple diseases in wheat, including black point, root rot, and leaf spot 4 , 11 , 27 . Therefore, plants have evolved complex strategies to allow rapid modulation of biological processes and cellular functions of an active defense response, such as increasing antioxidase activity and inducing the expression of various genes related to oxidative stress, including genes of pathogenesis-related (PR) proteins 26 – 28 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat

Gao

et al. 2021

Sci Rep

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Black point is a cereal disease caused by complex pathogens, of which the pathogenicity of Bipolaris sorokiniana is the most serious in wheat. Resistance to black point is quantitative in nature, and thus the mechanism is poorly understood. We conducted a comparative transcriptome analysis to identify differentially expressed genes (DEGs) in black point-slightly susceptible and -highly susceptible wheat lines at different timepoints following B. sorokiniana inoculation. DEGs associated with photosynthesis were upregulated in black point-slightly susceptible lines. The top Gene Ontology enrichment terms for biological processes were oxidation–reduction, response to cold, salt stress, oxidative stress, and cadmium ion; terms for cellular component genes were mainly involved in plasma membrane and cytoplasmic membrane-bounded vesicle, whereas those for molecular function were heme binding and peroxidase activity. Moreover, activities of antioxidant enzymes superoxide dismutase, catalase, and peroxidase were higher in slightly susceptible lines than those in highly susceptible lines (except peroxidase 12–24 days post-inoculation). Thus, resistance to B. sorokiniana-caused black point in wheat was mainly related to counteracting oxidative stress, although the specific metabolic pathways require further study. This study presents new insights for understanding resistance mechanisms of selected wheat lines to black point.

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Section: Discussionmentioning

confidence: 99%

Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat

Gao

et al. 2021

Sci Rep

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“…In recent years, transcriptome and metabolome analyses have provided a powerful comprehensive approach to assess the relationship of genotype, phenotype, and metabolite changes in plants challenged by abiotic and biotic stresses (Etalo et al, 2013;Mo et al, 2019;Ye et al, 2019;Hong et al, 2020). For example, transcriptional profiling combined with targeted metabolite quantification found that the levels of many genes and several metabolites in phenylpropanoid and shikimate pathways are significantly changed by the expression of WtsE, an effector secreted from Pantoea stewartii ssp.…”

Section: Introductionmentioning

confidence: 99%

Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response

Wang

et al. 2021

Front. Plant Sci.

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Maize is one of the major crops in the world; however, diseases caused by various pathogens seriously affect its yield and quality. The maize Rp1-D21 mutant (mt) caused by the intragenic recombination between two nucleotide-binding, leucine-rich repeat (NLR) proteins, exhibits autoactive hypersensitive response (HR). In this study, we integrated transcriptomic and metabolomic analyses to identify differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) in Rp1-D21 mt compared to the wild type (WT). Genes involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI) were enriched among the DEGs. The salicylic acid (SA) pathway and the phenylpropanoid biosynthesis pathway were induced at both the transcriptional and metabolic levels. The DAMs identified included lipids, flavones, and phenolic acids, including 2,5-DHBA O-hexoside, the production of which is catalyzed by uridinediphosphate (UDP)-dependent glycosyltransferase (UGT). Four maize UGTs (ZmUGTs) homologous genes were among the DEGs. Functional analysis by transient co-expression in Nicotiana benthamiana showed that ZmUGT9250 and ZmUGT5174, but not ZmUGT9256 and ZmUGT8707, partially suppressed the HR triggered by Rp1-D21 or its N-terminal coiled-coil signaling domain (CCD21). None of the four ZmUGTs interacted physically with CCD21 in yeast two-hybrid or co-immunoprecipitation assays. We discuss the possibility that ZmUGTs might be involved in defense response by regulating SA homeostasis.

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“…Combined approaches involving high-throughput transcriptome sequencing and whole-metabolome profiling are improving our understanding in plant-pathogen and plant-insect pest interactions [17][18][19][20]. With the limited amount of information available on the transcriptomic and metabolomic response of common bean to FSP infection, in this study, we employed a combined de novo transcriptome and metabolome analysis to obtain comprehensive insight into the defense responses of common bean to FSP.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

Chen¹,

Wu²,

He³

et al. 2020

Genes

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Common bean (Phaseolus vulgaris L.) is a major legume and is frequently attacked by fungal pathogens, including Fusarium solani f. sp. phaseoli (FSP), which cause Fusarium root rot. FSP substantially reduces common bean yields across the world, including China, but little is known about how common bean plants defend themselves against this fungal pathogen. In the current study, we combined next-generation RNA sequencing and metabolomics techniques to investigate the changes in gene expression and metabolomic processes in common bean infected with FSP. There were 29,722 differentially regulated genes and 300 differentially regulated metabolites between control and infected plants. The combined omics approach revealed that FSP is perceived by PAMP-triggered immunity and effector-triggered immunity. Infected seedlings showed that common bean responded by cell wall modification, ROS generation, and a synergistic hormone-driven defense response. Further analysis showed that FSP induced energy metabolism, nitrogen mobilization, accumulation of sugars, and arginine and proline metabolism. Importantly, metabolic pathways were most significantly enriched, which resulted in increased levels of metabolites that were involved in the plant defense response. A correspondence between the transcript pattern and metabolite profile was observed in the discussed pathways. The combined omics approach enhances our understanding of the less explored pathosystem and will provide clues for the development of common bean cultivars’ resistant to FSP.

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Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis

Cited by 29 publications

References 48 publications

Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat

Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat

Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response

Transcriptomic and Metabolomic Changes Triggered by Fusarium solani in Common Bean (Phaseolus vulgaris L.)

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