Disc-electrophoretic separation of water soluble proteins from scales of different forms of ichthyosis

Leonhardi, G.; Ross, L. U.; Neufahrt, A

doi:10.1007/bf00561417

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…Investigations of the soluble protein of pathological stratum corneum are few (8)(9)(10), because their significance is unclear, even though they appear to include most of the basic proteins in the interfilamentous matrix of the horny cells. Of these basic proteins, however, the characteristics of keratohyaline granule derivative protein have recently been thoroughly studied (19)(20)(21)(22)(23).…”

Section: Electrophoresis Of Keratin Fibrous Fractionsmentioning

confidence: 99%

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Fractionation and Characterization of the Human Epidermal Stratum Corneum in Keratinization Disorders

Yoshiike

Negi

Manabe

et al. 1982

The Journal of Dermatology

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Stratum corneum obtained from persons with a variety of keratinization disorders was fractionated into keratin fibers, water soluble proteins and cell membranes. SDS gel electrophoresis was used to study the keratin fibrous and water soluble fractions, and amino-acid analysis was employed for the membra~eousfractions.Electrophoresis of the keratin fibrous fractions obtained from cases of epidermolytic hyperkeratosis and psoriasis vulgaris revealed a significant decrease in the 55,000 dalton subunit was observed. Similarly, a reduction of the 73,000 dalton subunit was observed in samples from a case of keratoma climacterium. Minor variations from the normal were recorded from cases of ichthyosis vulgaris, lamellar ichthyosis, and palmoplantar keratosis (which included a Vomer and punctate type, and one with corneal dystrophy).The water soluble fractions from all the cases of keratinization disorders studied exhibited different densities for the individual polypeptides after electrophoresis.The most impressive changes were revealed by amino-acid analysis of the membraneous fractions. Half-cystine and proline were uniformly reduced in cases of ichthyosis vulgaris, lamellar ichthyosis, punctate palmo-plantar keratosis, palmoplantar keratosis with corneal dystrophy and keratoma climacterium, and the presence of an abnormal amino-acid, ornithine, was characteristic of epidermolytic hyperkeratosis and psoriasis vulgaris.

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Section: Electrophoresis Of Keratin Fibrous Fractionsmentioning

confidence: 99%

“…However, little research has been carried out on soluble proteins (8)(9)(10) and cell membranes (11) of abnormal stratum corneum. In this study, a very simple and practical procedure for fractionating human epidermal stratum corneum into keratin fibers, water soluble proteins, and cell membranes is presented.…”

Section: Introductionmentioning

confidence: 99%

Fractionation and Characterization of the Human Epidermal Stratum Corneum in Keratinization Disorders

Yoshiike

Negi

Manabe

et al. 1982

The Journal of Dermatology

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Presence of two typical DNA-binding nonhistone proteins in psoriatic scales contrary to normal human dermis, epidermis and horny layer

Reimer¹

1978

Arch Dermatol Res

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The composition of DNA-binding proteins (DBP) was shown to be tissue-specific and to vary at different stages of gene expression. As the accelerated epidermopoesis in psoriasis indicates changed gene activities, DBP of psoriatic scales were compared with those of normal human epidermis, dermis and horny layer. Each skin fraction is characterized by its own DBP pattern, indicating different cell species. 1. The DBP of normal human epidermis shows only a small accordance with the DBP of human dermis and implies their difference in origin, function and cell types. 2. Psoriatic scale DBP and epidermal DPB contain more corresponding proteins which can be deduced from the scale's origin from epidermis. However, the composition of all proteins differs to a great extent. This either occurred during parakeratotic keratinization or reflects differences of normal to psoriatic epidermis. Imposing for psoriatic scale DBP are two protein bands with molecular weights of 84,000 and 90,000 daltons. Evidently both are not present in the DBP of other skin layers. 3. The horny layer contains a very small amount of DBP which might represent DNases to a major part. The small DBP content in horny layer confirms the previous supposition of psoriatic scales, to be mostly derived from the preserved nuclei of the parakeratotic scale layer.

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Disc-electrophoretic separation of water soluble proteins from scales of different forms of ichthyosis

Cited by 2 publications

References 11 publications

Fractionation and Characterization of the Human Epidermal Stratum Corneum in Keratinization Disorders

Fractionation and Characterization of the Human Epidermal Stratum Corneum in Keratinization Disorders

Presence of two typical DNA-binding nonhistone proteins in psoriatic scales contrary to normal human dermis, epidermis and horny layer

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