Disaturated-phosphatidylcholine and Surfactant protein-B turnover in human acute lung injury and in control patients

Simonato, Manuela; Baritussio, Aldo; Ori, Carlo; Vedovelli, Luca; Rossi, Sandra; Massara, Lorenza Dalla; Rizzi, S.; Carnielli, Virgilio P.; Cogo, Paola

doi:10.1186/1465-9921-12-36

Cited by 25 publications

(34 citation statements)

References 39 publications

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“…Figure 3 reports SP-C isotopic enrichment curves in our patients together with SP-B curves obtained in the same patients in previous studies [18,21]. As shown in Fig.…”

Section: Kinetic Datamentioning

confidence: 78%

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Surfactant protein C metabolism in human infants and adult patients by stable isotope tracer and mass spectrometry

et al. 2014

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Surfactant protein C (SP-C) is deemed as the surfactant protein most specifically expressed in type II alveolar epithelial cells and plays an important role in surfactant function. SP-C turnover in humans and its meaning in the clinical context have never been approached. In this study, we used mass spectrometry to investigate SP-C turnover in humans. We studied four infants and eight adults requiring mechanical ventilation. All patients had no lung disease. Patients received a 24-h continuous infusion of (13)C-leucine as precursor of SP-C, and serial tracheal aspirates and plasma samples were obtained every 6 h till 48 h. SP-C was isolated from tracheal aspirates by sorbent-phase chromatography. (13)C-leucine SP-C enrichment could be successfully measured in three infant and in four adult samples by using mass spectrometry coupled with a gas chromatographer. Median SP-C fractional synthesis rate, secretion time, and peak time were 15.7 (14.1-27.5)%/day, 6.0 (4.7-11.5) h, and 24 (20-27) h. In conclusion, this study shows that it is feasible to accurately determine SP-C turnover in humans by stable isotopes.

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“…Figure 3 reports SP-C isotopic enrichment curves in our patients together with SP-B curves obtained in the same patients in previous studies [18,21]. As shown in Fig.…”

Section: Kinetic Datamentioning

confidence: 78%

“…Fractional synthesis rate (FSR), secretion time (ST), and peak time (PT) were calculated as previously reported [21,18]. Briefly, FSR was calculated by dividing the slope of the linear increase of the enrichment of SP-C by the plasma steady-state enrichment of free 13 C-leucine.…”

Section: Discussionmentioning

confidence: 99%

Surfactant protein C metabolism in human infants and adult patients by stable isotope tracer and mass spectrometry

et al. 2014

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“…BALF DSPC pools were significantly diminished in I lungs compared with the NI and control lungs. In ARDS patients we recently reported that DSPC amount in tracheal aspirates was markedly reduced and DSPC FSR was 3.1 times higher than in control patients (34), suggesting an overall compensatory DSPC synthetic mechanism that was still not sufficient to replace the DSPC lost during the acute phase of lung injury.…”

Section: Discussionmentioning

confidence: 99%

Pulmonary surfactant synthesis after unilateral lung injury in mice

Lamonica

Amigoni

Vedovelli

et al. 2014

Journal of Applied Physiology

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Aspiration pneumonitis can lead to alveolar surfactant dysfunction. We employed a murine model of unilateral aspiration to compare surfactant synthesis in the injured (I) and noninjured (NI) contralateral lung. Mice were instilled with hydrochloric acid in the right bronchus and, after 18 h, an intraperitoneal dose of deuterated water was administered as precursor of disaturated phosphatidylcholine (DSPC)-palmitate. Selected bronchoalveolar lavage fluid (BALF) was collected at scheduled time points and lungs were removed. We measured DSPC-palmitate synthesis in lung tissue and secretion in BALF by gas chromatography-isotope ratio mass spectrometry, together with total proteins and myeloperoxidase activity (MPO) by spectrophotometry. BALF total proteins and MPO were significantly increased in the I lungs compared with NI and naïve control lungs. The DSPC pool size was significantly lower in the BALF of the I lungs compared with naïve controls. DSPC synthesis was accelerated in the I and NI lungs. DSPC secretion of the I lungs was similar to their respective naïve controls, and it was markedly lower compared with their respective NI contralateral lungs. DSPC synthesis and secretion were faster, especially in the NI lungs, compared with naïve control lungs, as a possible compensatory mechanism due to a cross-talk between the lungs triggered by inflammation, hyperventilation, and/or undetermined type II cell reaction to the injury.

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“…DSPC and SP-B concentrations were expressed as percentage of total phospholipids and as concentrations in epithelial lining fluid [19].…”

Section: Analysis Of Tracheal Aspiratesmentioning

confidence: 99%

“…Fractional synthesis rate was calculated from the linear portion of the enrichment curve rise over time relative to the plateau enrichment of the respective precursors [19,20].…”

Section: Calculationsmentioning

confidence: 99%

Impaired surfactant protein B synthesis in infants with congenital diaphragmatic hernia

et al. 2012

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Pulmonary hypoplasia and hypertension account for significant morbidity and mortality in neonates with congenital diaphragmatic hernia (CDH). Whether CDH is associated with surfactant dysfunction remains controversial. Therefore, we measured disaturated phosphatidylcholine (DSPC) and surfactant protein (SP)-B concentration in tracheal aspirates and their synthesis rate in infants with CDH compared to infants without lung disease.2 H 2 O as a precursor of DSPC and 1-13 C-leucine as a precursor of SP-B were administered to 13 infants with CDH and eight controls matched for gestational age. DSPC and SP-B were isolated from tracheal aspirates, and their fractional synthesis rate was derived from 2 H and 13 C enrichment curves obtained by mass spectrometry. DSPC and SP-B amounts in tracheal aspirates were also measured. In infants with CDH, SP-B fractional synthesis rate and amount were 62¡27% and 57¡22% lower, respectively, than the value found in infants without lung disease (p,0.01 and p,0.05, respectively). There were no significant group differences in DSPC fractional synthesis rate and amount.Infants with CDH have a lower rate of synthesis of SP-B and less SP-B in tracheal aspirates. In these infants, partial SP-B deficiency could contribute to the severity of respiratory failure and its correction might represent a therapeutic goal.

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Disaturated-phosphatidylcholine and Surfactant protein-B turnover in human acute lung injury and in control patients

Cited by 25 publications

References 39 publications

Surfactant protein C metabolism in human infants and adult patients by stable isotope tracer and mass spectrometry

Surfactant protein C metabolism in human infants and adult patients by stable isotope tracer and mass spectrometry

Pulmonary surfactant synthesis after unilateral lung injury in mice

Impaired surfactant protein B synthesis in infants with congenital diaphragmatic hernia

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