Disassembly of Noncovalent Amphiphilic Polymers with Proteins and Utility in Pattern Sensing

Savariar, Elamprakash N.; Ghosh, Suhrit; Thayumanavan, S.

doi:10.1021/ja800164z

Cited by 118 publications

(96 citation statements)

References 18 publications

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“…This strategy represents a noncovalent method different from the ones proposed by Thayumanavan 17,18 and Ji. 20 Their strategies are more based on protein-induced disassembling of supramolecular assemblies and result in fluorescence quenching of the encapsulated fluorophores.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Protein Binding-Induced Surfactant Aggregation Variation: A New Strategy of Developing Fluorescent Aqueous Sensor for Proteins

Ding

Cao

et al. 2015

ACS Appl. Mater. Interfaces

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Novel strategies of developing fluorescent sensors for proteins are highly demanded. In this work, we particularly synthesized a cholesterol-derivatized pyrene probe. Its fluorescence emission is effectively tuned by the aggregation state of a cationic surfactant dodecyltrimethylammonium bromide (DTAB). The used probe/DTAB assemblies exhibit highly sensitive ratiometric responses to pepsin and ovalbumin egg (o-egg) with detection limits of 4.8 and 18.9 nM, respectively. The fluorescence changes indicate the protein-surfactant interaction leads to further aggregation of DTAB assemblies. The results from Tyndall effect and dynamic light scattering verify this assumption. The responses to pepsin and o-egg are due to their strong electrostatic or hydrophobic interaction with DTAB assemblies at pH 7.4. The present noncovalent supramolecular sensor represents a novel and simple strategy for sensing proteins, which is based on the encapsulated fluorophore probing the aggregation variation of the surfactant assemblies.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Protein Binding-Induced Surfactant Aggregation Variation: A New Strategy of Developing Fluorescent Aqueous Sensor for Proteins

Ding

Cao

et al. 2015

ACS Appl. Mater. Interfaces

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“…The I 392 /I 370 ratio of the pyrene uorescence emission spectra as a function of lauroylcholine concentration gave the CMC value of 0.612 mM at 20 C. According to the literature, surfactants can form supramolecular assemblies in aqueous solutions, and the critical aggregation concentration (CAC) of a surfactant could be reduced in the presence of counterions. 41,42 In this paper, PDI-DHA was used as a counterion to reduce the CAC of lauroylcholine due to its strong electrostatic interactions. In addition, 10 mM of PDI-DHA was preferentially prepared in the MOPS buffer solution (10 mM, pH 7.5), which is signicant for excimer formation.…”

Section: Resultsmentioning

confidence: 99%

A ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening based on supramolecular assembly induced monomer–excimer emission transition of a perylene probe

Zhou

Hussain

et al. 2018

RSC Adv.

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“…These particles disassembled and released drug in response to phosphorylation catalysed by PKA. The micellization of the complex of the polymer poly(potassium acrylate) (PPA) and the surfactant cetyltrimethylammonium bromide (CTAB), using the fluorescent pyrene as a guest molecule, resulted in an enzyme responsive system [100,101].…”

Section: Enzyme-responsive Micellesmentioning

confidence: 99%

Stimuli-Responsive Polymers and Their Applications in Nanomedicine

et al. 2012

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This review focuses on smart nano-materials built of stimuli-responsive (SR) polymers and will discuss their numerous applications in the biomedical field. The authors will first provide an overview of different stimuli and their corresponding, responsive polymers. By introducing myriad functionalities, SR polymers present a wide range of possibilities in the design of stimuli-responsive devices, making use of virtually all types of polymer constructs, from self-assembled structures (micelles, vesicles) to surfaces (polymer brushes, films) as described in the second section of the review. In the last section of this review the authors report on some of the most promising applications of stimuli-responsive polymers in nanomedicine. In particular, we will discuss applications pertaining to diagnosis, where SR polymers are used to construct sensors capable of selective recognition and quantification of analytes and physical variables, as well as imaging devices. We will also highlight some examples of responsive systems used for therapeutic applications, including smart drug delivery systems (micelles, vesicles, dendrimers...) and surfaces for regenerative medicine.

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Disassembly of Noncovalent Amphiphilic Polymers with Proteins and Utility in Pattern Sensing

Cited by 118 publications

References 18 publications

Protein Binding-Induced Surfactant Aggregation Variation: A New Strategy of Developing Fluorescent Aqueous Sensor for Proteins

Protein Binding-Induced Surfactant Aggregation Variation: A New Strategy of Developing Fluorescent Aqueous Sensor for Proteins

A ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening based on supramolecular assembly induced monomer–excimer emission transition of a perylene probe

Stimuli-Responsive Polymers and Their Applications in Nanomedicine

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