Disassembling bacterial extracellular matrix with DNase-coated nanoparticles to enhance antibiotic delivery in biofilm infections

Baelo, Aida; Levato, Riccardo; Julián, Esther; Crespo, Anna; Astola, José; Gavaldá, Joan; Engel, Elisabeth; Mateos‐Timoneda, Miguel A.; Torrents, Eduard

doi:10.1016/j.jconrel.2015.04.028

Cited by 193 publications

(139 citation statements)

References 41 publications

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“…Once biofilm matrix with extra polymeric substances is in place, impermeable property inhibits the penetration of therapeutic agents. Nanoparticles are able to penetrate the biofilm owing to their nano-scale particle size [57, 58]. …”

Section: Discussionmentioning

confidence: 99%

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

et al. 2017

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Propolis obtained from bee hives is a natural substance with antimicrobial properties. It is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of Malaysian propolis were prepared. Both the extracts displayed antimicrobial and anti-biofilm properties against Enterococcus faecalis, a common bacterium associated with hospital-acquired infections. High performance liquid chromatography (HPLC) analysis of propolis revealed the presence of flavonoids like kaempferol and pinocembrin. This study investigated the role of propolis developed into nanoparticles with chitosan for its antimicrobial and anti-biofilm properties against E. faecalis. Bacteria that grow in a slimy layer of biofilm are resistant to penetration by antibacterial agents. The use of nanoparticles in medicine has received attention recently due to better bioavailability, enhanced penetrative capacity and improved efficacy. A chitosan-propolis nanoformulation was chosen based on ideal physicochemical properties such as particle size, zeta potential, polydispersity index, encapsulation efficiency and the rate of release of the active ingredients. This formulation inhibited E. faecalis biofilm formation and reduced the number of bacteria in the biofilm by ~90% at 200 μg/ml concentration. When tested on pre-formed biofilms, the formulation reduced bacterial number in the biofilm by ~40% and ~75% at 200 and 300 μg/ml, respectively. The formulation not only reduced bacterial numbers, but also physically disrupted the biofilm structure as observed by scanning electron microscopy. Treatment of biofilms with chitosan-propolis nanoparticles altered the expression of biofilm-associated genes in E. faecalis. The results of this study revealed that chitosan-propolis nanoformulation can be deemed as a potential anti-biofilm agent in resisting infections involving biofilm formation like chronic wounds and surgical site infections.

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Section: Discussionmentioning

confidence: 99%

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

et al. 2017

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“…71 Ciproflaxin loaded poly(lactic-co-glycolic acid) nanoparticles modified with DNase I were investigated by Torrents and co-workers for the eradication of established biofilms. 63 While the particles were not able to prevent biofilm formation, from planktonic bacteria, they successfully reduced biofilm mass and size (Fig. 4A).…”

Section: Polymer Nanoparticles and Hydrogelsmentioning

confidence: 98%

“…Repeated administration over 3 days reduced 95% and eradicated more than 99.8% of established biofilms. 63 Polymeric nanofibers, composed of PLGA and PCL, were developed by Ashbaugher et al for local co-delivery of a combination of antibiotics. 80 The release can be adjusted by varying the PLGA/PCL polymer ratio and proved to be highly effective in preventing medical device infections in patients.…”

Section: Polymer Nanoparticles and Hydrogelsmentioning

confidence: 99%

Advancements on the molecular design of nanoantibiotics: current level of development and future challenges

Jijie

Barras

Teodorescu

et al. 2017

Mol. Syst. Des. Eng.

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Abstract.Numerous antimicrobial drugs have been developed and commercialized to kill and inhibit the growth of pathogenic microbes. The therapeutic efficiency of these drugs has however become often inadequate for the treatment of microbial infections, the reasons being multiple. Oral administration results often in only partial absorption and up to 50% of the active compound can be excreted in the urine. Furthermore, antibiotic therapy is also frequently accompanied by gastrointestinal complications, including vomiting, nausea and diarrhea. From a therapeutic point of view, the low solubility of antibiotics in lipid membranes presents a limitation for rapid and efficient treatment of infections. The preferred route for the delivery of antimicrobial drugs is the oral one; oral formulations capable of extending the duration of drug delivery and minimizing side effects have received much attention. The combination of antimicrobial agents with nanomaterials has been seen as a particularly promising strategy to overcome these limitations. Particulate formulations have proven their efficiency in achieving better pharmacokinetic profiles and improving the bio availability of several antibiotics. Antibiotic modified particles have shown their capability to protect some of the antimicrobial drugs from stomach acid and first-pass metabolisms in the gastrointestinal tract. Likewise, particle formulations can increase the circulation times of a drug and the local concentration gradient across absorptive cells. The present review describes the current status of different types of antibiotic loaded nano-systems. Key principles such as antibiotic loading, the release mechanism and the mode of action involved in pathogen killing or inhibition will be discussed in more detail. It is hoped that the general knowledge obtained here will help in the generation of advanced nanomaterials loaded with antimicrobials agents.Post-print 2

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“…The present study was similar to that of reported by Visha et al [23].The difference in the reported sizes of nanoparticles could be explained by the variation in the strain used as bacterial proteins play a major role in controlling the size and shape of nanoparticles .In the present study it was found that SeNPs was effective against biofilm forming carbohydrate content reduction by SeNPs was found to be in all microorganisms respectively. The small size of nanoparticles alone [25]or combined with antibiotic activity of metals [26] has been employed by other researchers as well for the disruption of extracellular matrix in microbial biofilms.…”

Section: Bacillus Subtilismentioning

confidence: 99%

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2018

RJLBPCS

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Bacteria in biofilms are innately more resistant to existing antimicrobial agents owing to protective covering of exopolysaccharides around microbial colonies. The eradication of biofilm is difficult thereby accentuating the need to develop alternative interventions. The present study is concerned with the development and characterization of selenium nanoparticles (SeNPs) prepared by probiotics against biofilms. The nanoparticles were characterized by TEM. The antimicrobial study showed that the generated nanoparticles were more effective against biofilm forming micro-organisms. As biofilms are generally composed of extracellular proteins, and polysaccharides;SeNPs also reduced the protein as well as carbohydrates content crucial to biofilm formation and antimicrobial resistance. KEYWORDS: MATERIALS AND METHODSCommercially availableSporolac manufactured by Uni Sankyo Ltd. containing around 150 million spores ofLactobacillus sporogenesin 1g sachet was used in the study, Sodium selenite, NutrientBroth, Nutrient agar, MRS broth, YEPD agar, YEPD broth, Trypticase soy broth manufactured byHiMedia. All the reagents of standard grade were used in the study. Micro organismsFor evaluating the antimicrobial activity of selenium nanoparticles different microbial strains Congo Red Agar Method (CRA):Prepared 250 gm nutrient agar medium andCongo red stain as concentrated aqueous solution separately, mixed and autoclaved at 121˚ C for 15 minutes. Plates were inoculated with test organism and incubated at 37 ͦ C for 24 to 48 hours aerobically. Black colonies with a dry crystalline consistency indicated biofilm production; weak producers usually remained pink, though occasional darkening at the center of colonies was observed [19]. Tissue culture plate method (TCP):Isolates from fresh agar plates were inoculated on trypticase soy broth with 1% glucose (TSB media and incubated for 18 hours at 37°C and then diluted 1 in 100 with fresh medium. Individual wells of sterile polystyrene, 96 wells-flat bottom tissue culture plates were filled with 0.2 ml aliquots of the diluted cultures and only broth served as control to check the sterility and non-specific binding of the media. The tissue culture plates were incubated for 24 hours at 37°C.After incubation, the content from each well was gently removed by tapping the plates. The plates were gently submerged

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Disassembling bacterial extracellular matrix with DNase-coated nanoparticles to enhance antibiotic delivery in biofilm infections

Cited by 193 publications

References 41 publications

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

Advancements on the molecular design of nanoantibiotics: current level of development and future challenges

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