Disappearance of acetic acid from the bovine reticulorumen at basal and elevated concentrations of acetic acid1

Peters, J. P.; Shen, R. Y. W.; Robinson, J. A.

doi:10.2527/1992.7051509x

Cited by 13 publications

(8 citation statements)

References 14 publications

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“…This result confirms previous data obtained with the same method by Weigand et al (1972a,b), Thorlacius and Lodge (1973) and Oshio and Tahata (1984) who showed that VFA absorption rate (% of VFAs infused) was constant when their ruminal concentration increased, provided the latter was lower than 130 mM. Peters et al (1992) obtained the same result using marked VFAs. Only Dijkstra et al (1993) observed a saturation of VFA absorption when VFA concentration increased.…”

Section: Effect Of Available Vfassupporting

confidence: 91%

Effects of level of intake and of available volatile fatty acids on the absorptive capacity of sheep rumen

Doreau

Ferchal

Beckers

1997

Small Ruminant Research

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The objective of this experiment was to determine the respective effects of level of intake and of the amount of volatile fatty acids (VFAs) present in the rumen on VFA ruminal absorption. In a factorial design, six ewes received a hay diet either at 126 or at 73% of energy maintenance requirements. Their capacity of absorption of VFAs was evaluated by the method of temporarily isolated rumens, in which the rumen was filled with solutions containing high or low amounts of VFAs. Rate of absorption (amount of VFAs absorbed per hour) was significantly higher, by 32% on average, in sheep fed the high intake than in sheep fed at low intake. The proportion of initial VFAs absorbed did not depend on the concentration of VFAs introduced in the rumen, so that the amount of VFAs absorbed varied as the concentration of VFAs introduced in the rumen. The decrease in intake resulted in a decrease by 32% in the rate of absorption of total VFAs. No interaction between concentration of VFAs and previous intake was observed. This suggests that the capacity of the rumen to absorb VFAs is reduced at low intake even if low amounts of VFAs are available.

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Section: Effect Of Available Vfassupporting

confidence: 91%

Effects of level of intake and of available volatile fatty acids on the absorptive capacity of sheep rumen

Doreau

Ferchal

Beckers

1997

Small Ruminant Research

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“…The importance of the undisturbed metabolism of SCFA for the health and performance of ruminants can hardly be overestimated, with their production in cattle reaching more than 100 mol/day and accounting for more than 75% of the total metabolizable energy in these animals [5,8,9]. Most of the SCFA are absorbed by the ruminal epithelium [10][11][12], and although a large fraction of butyrate is metabolized intracellularly [4,[13][14][15], both in vivo [14][15][16] and in vitro studies [17][18][19][20][21] have demonstrated that considerable amounts of acetate and propionate cross the basolateral membrane of the rumen in an unmetabolized state.…”

Section: Introductionmentioning

confidence: 98%

Cultured ruminal epithelial cells express a large-conductance channel permeable to chloride, bicarbonate, and acetate

Stumpff

Martens

Bilk

et al. 2008

Pflugers Arch - Eur J Physiol

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The absorption of short-chain fatty acids (SCFA) from the rumen requires efficient mechanisms for both apical uptake and basolateral extrusion. Previous studies suggest that the rumen expresses a basolateral chloride conductance that might be permeable to SCFA. In order to characterize this conductance in more detail, isolated cultured ruminal epithelial cells were studied with the patch-clamp technique, revealing a whole-cell conductance with p(Cl(-)) approximately p(NO(3) (-)) > p(HCO(3) (-)) > p(acetate(-)) > p(gluconate(-)). Currents could be blocked by diisothiocyanato-stilbene-2,2'-disulfonic acid (1 mmol l(-1) > 100 micromol l(-1)), 5-nitro-2-(3-phenylpropyl-amino)benzoic acid (50 micromol l(-1)), niflumic acid (100 micromol l(-1)), and p-chloromercuribenzoate (1 mmol l(-1)). Single-channel conductance was 350 +/- 7 pS for chloride and 142 +/- 7 pS for acetate. Open probability could be fitted with a three-state gating model. We propose a role for this channel in mediating the permeation of chloride, bicarbonate, and acetate across the basolateral membrane of the ruminal epithelium.

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“…VFA absorption through the rumen wall of steers represents 65 to 87% of its intraruminal production (Peters et al, 1990(Peters et al, , 1992. Considerable amounts of intraruminally produced VFA are metabolized during absorption by the rumen epithelium (Kristensen et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Influence of dietary concentrate to forage ratio on the development of rumen mucosa in calves

Žitňan

Voigt

Schönhusen

et al. 1998

Archiv für Tierernaehrung

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Effects of structural and non-structural carbohydrates on the development of rumen fermentation and ruminal mucosa in calves were examined during the weaning period. Barley/soybean meal (SBM) group was fed a concentrate starting from 2 weeks of age, whereas alfalfa group received a mixture of concentrate and alfalfa hay in which the proportion of the latter was gradually increased from 20% to 70% between weeks 2 and 9 of age. The total volatile fatty acid concentration in rumen fluid of calves increased with age, but at 9 weeks there were no significant differences between the two diets (barley/SBM group 153 mmol/l, alfalfa group 150 mmol/l). Rumen papillae at 9 weeks of age, as compared to 6 weeks of age, were longer and fewer in number per square centimetre mucosa, with larger cut surface. This resulted in a higher surface of papillae per square centimetre mucosa at 9 weeks (barley/SBM group 286 mm2/cm2, alfalfa group 245 mm2/cm2) than at 6 weeks of age (barley/SBM group 217 mm2/cm2, alfalfa group 198 mm2/cm2). At 9 weeks of age, the pH (barley/SBM 5.0, alfalfa 5.7), the acetate to propionate ratio (barley/SBM 2.2, alfalfa 3.2) as well as the length of the papillae in the ventral ruminal sac (barley/SBM 1.96 mm, alfalfa 2.37 mm) were increased in the alfalfa group when compared to the barley/SBM group (P < 0.1). In the former group, the proportion of butyrate revealed significantly increased values at 4 and 6 weeks of age. In animals of the barley/SBM group at 9 weeks of age, characteristic protrusions with proliferated thick epithelium occurred on the papillae and increased the surface for absorption. On the epithelium (Stratum corneum) desquamating cells with parakeratosis could be observed. In the alfalfa group the papillae of the ventral ruminal sac were longer, without protrusions. The morphotypes of the adhering rumen microflora differed between the groups. It can be concluded that feeding greater amounts of non-structural carbohydrates increases the surface for absorption of the rumen epithelium in calves. The absence of hyperkeratosis and rumenitis in the barley/SBM group indicated that there is no reason to limit high starch diets in the early weaning period of calves.

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Disappearance of acetic acid from the bovine reticulorumen at basal and elevated concentrations of acetic acid1

Cited by 13 publications

References 14 publications

Effects of level of intake and of available volatile fatty acids on the absorptive capacity of sheep rumen

Effects of level of intake and of available volatile fatty acids on the absorptive capacity of sheep rumen

Cultured ruminal epithelial cells express a large-conductance channel permeable to chloride, bicarbonate, and acetate

Influence of dietary concentrate to forage ratio on the development of rumen mucosa in calves

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