Disagreement and Controversy in History

Levich, Marvin

doi:10.2307/2504334

Cited by 3 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This cell-flow method allows for the equilibration of all receptors on the cell surface with ligands within 4−10 ms. Glutamate was applied by the cell-flow method to establish the position of the cell that gave the largest maximum current amplitude. The current amplitude was then corrected for receptor desensitization that occurred during the equilibration of the cell surface receptors with glutamate by using eq 1 ( , ), which is based on the theory of the flow of solutions over spherical objects ( , ). To take into account the uneven rate at which the ligand solution flows over the cell surface, we divide the current time course into constant time intervals (we have used 3 ms) and then correct the current for the desensitization that occurs during each time interval Δ t .…”

Section: Methodsmentioning

confidence: 99%

Mechanism of Glutamate Receptor-Channel Function in Rat Hippocampal Neurons Investigated Using the Laser-Pulse Photolysis (LaPP) Technique

Nowak

Gee

et al. 2002

Biochemistry

View full text Add to dashboard Cite

Ionotropic glutamate receptors are members of a large family of plasma membrane proteins expressed by cells of the nervous system. Upon binding glutamate, the receptors transiently open transmembrane channels that allow the entry of sodium ions. The resulting changes in the transmembrane potential of the cell initiates a process that is involved in signal transmission to another cell. The binding of glutamic acid triggers the channel opening in the microsecond time domain and the reversible inactivation (desensitization) of the receptors in the millisecond time region. The channel-opening mechanism of glutamate receptors was investigated in rat hippocampal neurons voltage-clamped to -60 mV at room temperature and pH 7.4. Two rapid chemical reaction techniques were used: (1) a cell-flow method with a 4-10 ms time resolution to apply L-glutamate and (2) a laser-pulse photolysis technique to release glutamate from gamma-O-(alpha-carboxy-2-nitrobenzyl)glutamate (alphaCNB-caged L-glutamate) with a time constant of 30 micros. The rate and equilibrium constants for channel opening were determined. The results are consistent with the receptor binding two molecules of glutamic acid before the channel opens, with an apparent dissociation constant of 600 microM. Channel opening and closing rate constants, k(op) and k(cl), were determined to be (9.5 +/- 1) x 10(3) s(-1) and (1.1 +/- 0.1) x 10(3) s(-1), respectively. The value of the channel-opening equilibrium constant, Phi (=k(op)/k(cl)), was 8.6 when determined by laser-pulse photolysis and 6.6 in cell-flow experiments. The results suggest that there are at least two forms of glutamate receptors in rat hippocampal neurons that desensitize with different rates. At a concentration of 500 microM glutamate, 80% of the receptors desensitized with a rate of approximately 200 s(-1) and 20% with a rate of approximately 50 s(-1).

show abstract

Section: Methodsmentioning

confidence: 99%

Mechanism of Glutamate Receptor-Channel Function in Rat Hippocampal Neurons Investigated Using the Laser-Pulse Photolysis (LaPP) Technique

Nowak

Gee

et al. 2002

Biochemistry

View full text Add to dashboard Cite

show abstract

“…Whilst there exists an extensive literature on this subject [1][2][3][4][5] , the focus of earlier publications has been on local continuum descriptions. Subsequently, there have been several publications on mesoscopic 6,7 and atomistic [8][9][10] simulations of phoretic flows, but there is a shortage of simulations on model systems that are simple enough to allow quantitative comparison between analytical theory and simulation.…”

Section: Introductionmentioning

confidence: 99%

Comparing theory and simulation for thermo-osmosis

Proesmans

Frenkel

2019

The Journal of Chemical Physics

View full text Add to dashboard Cite

We report a numerical study of thermo-osmotic slip, i.e. the particle flux induced by a thermal gradient along a solid-fluid interface. To facilitate comparison with theory, we consider a model of an ideal but viscous gas. We compare three numerical routes to obtain the slip coefficient: 1. by using the Onsager reciprocity relations 2. by using the appropriate Green-Kubo relation 3. via the excess enthalpy. The numerical results are found to be mutually consistent, and to agree with the theoretical prediction based on the assumption that hydrodynamics and thermodynamics are locally valid.

show abstract

The Nonlinear Analysis of Chiral Medium

Волобуев¹

2019

Chirality From Molecular Electronic States

View full text Add to dashboard Cite

The principle of calculation of a plate from a metamaterial with inductive type chiral inclusions is submitted. It is shown that distribution of an electromagnetic wave to such substance can be investigated with the help of introduction of a chiral parameter and on the basis of a detailed method of calculation. By comparison of two methods the dependence of chiral parameter from frequency of electromagnetic radiation falling on a plate is found. With the help of a detailed method the nonlinear equation for potential on the chiral plate is found. It is shown that this equation has solutions as solitary and standing waves but not running waves. The analysis of the received solutions of the nonlinear equation is carried out.

show abstract

Disagreement and Controversy in History

Cited by 3 publications

References 0 publications

Mechanism of Glutamate Receptor-Channel Function in Rat Hippocampal Neurons Investigated Using the Laser-Pulse Photolysis (LaPP) Technique

Mechanism of Glutamate Receptor-Channel Function in Rat Hippocampal Neurons Investigated Using the Laser-Pulse Photolysis (LaPP) Technique

Comparing theory and simulation for thermo-osmosis

The Nonlinear Analysis of Chiral Medium

Contact Info

Product

Resources

About