Disaggregation of silver nanoparticle homoaggregates in a river water matrix

Metreveli, George; Philippe, Allan; Schaumann, Gabriele E.

doi:10.1016/j.scitotenv.2014.11.058

Cited by 73 publications

(47 citation statements)

References 61 publications

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“…The source, preparation and characterization of the citrate‐stabilized AgNP used in this study were described in detail by Metreveli et al () and Grün et al (). Briefly, citrate stabilized AgNP of two different sizes (30 and 70 nm) were used for the effect assessment on Acanthamoeba .…”

Section: Methodsmentioning

confidence: 99%

“…The mean hydrodynamic particle diameters (z‐average; h d ) as determined by dynamic light scattering (Delsa Nano C Particle Analyser, Beckman Coulter) were 32 ± 3 nm (AgNP30) and 97 ± 3 nm (AgNP70) with polydispersity indices of 0.4 ± 0.02 and 0.2 ± 0.01 for AgNP30 and AgNP70, respectively. The AgNP30 stock solution had a ζ‐potential of –50 mV at pH 7 and h d of 40 ± 0.4 nm ( Metreveli et al, ). AgNO 3 and trisodium citrate (Na 3 C 6 H 5 O 7 ) were used as internal controls in both assays.…”

Section: Methodsmentioning

confidence: 99%

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Assessment of the effect of silver nanoparticles on the relevant soil protozoan genus Acanthamoeba

Grün

Scheid

Hauröder

et al. 2017

J. Plant Nutr. Soil Sci.

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Silver nanoparticles (AgNP) are used in a broad range of consumer products and industrial applications. During the regular product life cycle and disposal, AgNP are continuously released into the environment. Hence, the aim of this study was to investigate the potential ecotoxicological effects of AgNP exposure on amoebae. The Acanthamoeba castellanii ATCC 30234 strain and environmental isolate Acanthamoeba strain C5/2, which are both affiliated with genotype T4, were chosen as representatives of ecologically important soil protozoan organisms. The amoebae were exposed to citrate‐stabilized AgNP (30 and 70 nm in size) for 24 h and 96 h at concentrations ranging from 600 µg L−1 to 20 mg L−1. A newly adopted cell culture based microscopic assay was applied to assess the adherence ability of the amoeba trophozoites. The general metabolic activity of Acanthamoeba was determined to be a second independent endpoint by means of intracellular reduction of the redox dye AlamarBlue®. The fate of AgNP within the amoebae and test solutions was visualized by light‐ and transmission electron microscopy (TEM). Both Acanthamoeba strains showed a significant dose‐dependent decrease of adherence ability (p < 0.04) and metabolic activity (p < 0.01) after 96 h of AgNP exposure. The environmental Acanthamoeba strain C5/2 lost both its adherence ability and metabolic activity at lower AgNP concentrations than the type strain, indicating a higher sensitivity to ionic silver. This was confirmed by the application of AgNO3, provoking a higher effect level in strain C5/2. AgNP was visualized intracellularly by transmission electron microscopy within the cytoplasm of Acanthamoeba. This is the first report to show the ecotoxicological effects of short‐term AgNP exposure on the soil protist Acanthamoeba, causing both changes in the adherence ability and metabolic activity of this amoeba. This combined approach may be a powerful tool in the future for predicting potential harmful ecotoxicological effects of AgNP exposure using soil protozoans.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Assessment of the effect of silver nanoparticles on the relevant soil protozoan genus Acanthamoeba

Grün

Scheid

Hauröder

et al. 2017

J. Plant Nutr. Soil Sci.

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“…Although DLS has serious limitations for sizing nanoparticle suspensions in complex matrices, it is very valuable to monitor aggregation behaviours. For example, aggregation of Ag NPs exposed to human synthetic stomach fluid [103], gastric juices [104] or river water [105] has been studied by DLS. In addition, this technique has also been used to proof the reliability of AF4 data in consumer products, although some results were biased to larger particle diameters respect to AF4 results [106].…”

Section: Light Scattering Techniquesmentioning

confidence: 99%

“…Thus larger particles are transported faster than smaller ones, as they spend less time near the edges of the capillaries [181]. [181,182], Ag NPs in natural river waters [105,183] and synthetic surface waters [184], and TiO 2 and ZnO NPs in commercial sunscreens [184]. A pre-treatment based on the aqueous extraction with a surfactant (Triton-X 100) and a 1 µm filtration was performed before the analysis of sunscreens by HDC.…”

Section: Hydrodynamic Chromatographymentioning

confidence: 99%

“…A pre-treatment based on the aqueous extraction with a surfactant (Triton-X 100) and a 1 µm filtration was performed before the analysis of sunscreens by HDC. From the two columns commercially available, the column with a separation range of the 5-300 nm is used preferentially [181][182][183][184][185] to the 20-1200 nm [105,184]. Poor resolution and coating surface effects on elution time have been observed for Au NPs in the range from 5 to 100 nm [185], which may prove problematic in complex matrices since NPs surface is likely to be modified, affecting their retention behaviour, as pointed out by these authors.…”

Section: Hydrodynamic Chromatographymentioning

confidence: 99%

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Detection, characterization and quantification of inorganic engineered nanomaterials: A review of techniques and methodological approaches for the analysis of complex samples

Laborda

Bolea

Cepriá

et al. 2016

Analytica Chimica Acta

325

170

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The increasing demand of analytical information related to inorganic engineered nanomaterials requires the adaptation of existing techniques and methods, or the development of new ones.The challenge for the analytical sciences has been to consider the nanoparticles as a new sort of analytes, involving both chemical (composition, mass and number concentration) and physical information (e.g. size, shape, aggregation). Moreover, information about the species derived from the nanoparticles themselves and their transformations must also be supplied. Whereas techniques commonly used for nanoparticle characterization, such as light scattering techniques, show serious limitations when applied to complex samples, other well-established techniques, like electron microscopy and atomic spectrometry, can provide useful information in most cases. Furthermore, separation techniques, including flow field flow fractionation, capillary electrophoresis and hydrodynamic chromatography, are moving to the nano domain, mostly hyphenated to inductively coupled plasma mass spectrometry as element specific detector.Emerging techniques based on the detection of single nanoparticles by using ICP-MS, but also coulometry, are in their way to gain a position. Chemical sensors selective to nanoparticles are in their early stages, but they are very promising considering their portability and simplicity.Although the field is in continuous evolution, at this moment it is moving from proofs-of-2 concept in simple matrices to methods dealing with matrices of higher complexity and relevant analyte concentrations. To achieve this goal, sample preparation methods are essential to manage such complex situations. Apart from size fractionation methods, matrix digestion, extraction and concentration methods capable of preserving the nature of the nanoparticles are being developed. This review presents and discusses the state-of-the-art analytical techniques and sample preparation methods suitable for dealing with complex samples. Single-and multimethod approaches applied to solve the nanometrological challenges posed by a variety of stakeholders are also presented.

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Surface and Mechanical Properties of Nanoparticulate Resin Coatings and Their Toxicological Characterization

et al. 2016

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A process chain to produce and characterize nanoparticulate, hydrophobic resin coatings was investigated. The investigated process stain could be used for sanitary applications, for instance. Nanocomposites might have a potential risk to humans and the environment. Thus, not only the manufacturing process, but also the achievable product properties were examined. Toxicological investigations with regard to the nanoparticle sizes and solid contents used were performed by investigating the impact of the nanoparticles on lung (A549 cells) and liver (HepG2 cells) cells. Results included showed that by using modified alumina the product properties could be improved in terms of hydrophobicity and mechanical properties and that alumina particles were nontoxic. However, cell viability was slightly affected by the nanocomposite extracts, depending on the applied particle size and concentration in the nanocomposites.

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Disaggregation of silver nanoparticle homoaggregates in a river water matrix

Cited by 73 publications

References 61 publications

Assessment of the effect of silver nanoparticles on the relevant soil protozoan genus Acanthamoeba

Assessment of the effect of silver nanoparticles on the relevant soil protozoan genus Acanthamoeba

Detection, characterization and quantification of inorganic engineered nanomaterials: A review of techniques and methodological approaches for the analysis of complex samples

Surface and Mechanical Properties of Nanoparticulate Resin Coatings and Their Toxicological Characterization

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