Disaccharide-polyethylenimine organic nanoparticles as non-toxic in vitro gene transporters and their anticancer potential

Singh, Reena; Kumar, Pradeep

doi:10.1016/j.bioorg.2021.104918

Cited by 4 publications

(5 citation statements)

References 60 publications

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“…Cytotoxicity of HA, HD-2, HD-2(TG) and TG was determined by the standard MTT assay [47][48][49] on HepG2, HeLa and HEK293 cells. In a culture plate, cells were treated with HA, HD-2, HD-2(TG) and TG at various concentrations (i.e.…”

Section: Mtt Assaymentioning

confidence: 99%

“…In vitro cellular uptake studies Like MTT assay, here, HeLa cells were treated with fluorescein-HD-2 and fluorescein-HD-2(TG) samples at IC 50 values, i.e. 100.38 and 119.22 μg, 48,49 and incubated for various time points, i.e. 0.5, 1.5, 4 and 8 h. ImageJ software was used for determination of collected total cell fluorescence (CTCF) values.…”

Section: Preparation Of Fluorescein-labeled Hd Nanoparticlesmentioning

confidence: 99%

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Design, fabrication and evaluation of amphiphilic hyaluronic acid conjugates as efficient carriers of 6‐thioguanine for in vitro anticancer drug delivery applications

Deka

Singh

Verma

et al. 2022

Polymer International

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Nanoparticle‐based polymeric carriers are highly desirable for efficient delivery of hydrophobic drugs. The development of nanocarriers with high encapsulation efficiency synthesized by a simple emulsification technique has become a challenging task with their controlled and sustained release ability at disease sites. Here, we have synthesized a series of hydrophobically modified hyaluronic–dodecylamide (HD) conjugates by coupling dodecylamine onto hyaluronic acid in varying amounts and characterized them using physicochemical techniques. The synthesized HD conjugates, in an aqueous medium, self‐assembled into nanoparticles of size in the range of ca 189–355 nm with hydrophilic surface and hydrophobic core. 6‐Thioguanine (TG) was used as a model drug for encapsulation into the hydrophobic core of the conjugate (HD‐2) by the standard emulsification technique with high encapsulation efficiency. Spectrophotometric analysis revealed that the drug‐loaded nanoconjugate showed a sustained release of drug over a period of time and also showed enzymatic responsiveness towards hyaluronidase. In vitro experiments were performed with HEK293, HeLa and HepG2 cells and it was observed that drug‐loaded nanoparticles were more toxic towards cancerous cells as compared with free TG. These results advocate that the reported system, being biocompatible, could be used as promising anticancer drug delivery system. © 2022 Society of Industrial Chemistry.

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Section: Mtt Assaymentioning

confidence: 99%

Section: Preparation Of Fluorescein-labeled Hd Nanoparticlesmentioning

confidence: 99%

Design, fabrication and evaluation of amphiphilic hyaluronic acid conjugates as efficient carriers of 6‐thioguanine for in vitro anticancer drug delivery applications

Deka

Singh

Verma

et al. 2022

Polymer International

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“…Alginate-nanocellulose Delivery of the drug Ibuprofen to be used as pain killer for multiple disorders [7] Glycol chitosan Photodynamic antitumor therapy via ROS sensitization [8] Carboxymethyl tamarind kernel polysaccharide Drug delivery in Ophthalmic diseases [9] Angelica sinensis polysaccharide hepatoma-targeted delivery of therapeutic drug doxorubicin for liver cancer [10] Ginseng polysaccharides Fluorescent labeling and study of immunomodulatory mechanism [11] Chitosan Bioimaging and killing of cancer cells [12] Astragalus polysaccharide Treatment of sepsis induced myocardial dysfunction via TLR4/NF-κB signaling pathway inhibition [13] Carboxymethyl chitosan and oxidized alginate Bone tissue engineering [14] Disaccharide lactose, trehalose and sucrose Delivery of antimicrobial peptide [15] Sucrose and trehalose Gene transfer and anticancer activity [16]…”

Section: Polysaccharidementioning

confidence: 99%

Carbohydrate-Based Nanoparticles in Therapeutics and Biomedicine

Verma

2022

CTBEB

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Nanotechnology is a new emerging technology that is actively used in modern biomedicine and research. Of the various types of NPs, carbohydrate/saccharide-based NPs find their application in the therapy and diagnosis of various disorders and diseases due to their natural origin and the importance of carbohydrate components in biological processes. Biological permeability and natural glycans provide various advantages for their use in the field of medicine.

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“…Nonviral vectors with easy preparation process, low cost, and good compatibility can avoid the potential immunogenicity and carcinogenicity of viral vectors. 31 Cationic liposome is an ideal nonviral vector; however, the cytotoxicity and low efficiency in vivo always restrict its application. 32,33 Inclusion of hydrophobic poly(lactide-co-glycolide) (PLGA) inner core inside of the lipid membrane can contribute to pack water-insoluble drug and avoid drug leakage.…”

Section: Introductionmentioning

confidence: 99%

“…At present, gene delivery vectors include viral and nonviral vectors. Nonviral vectors with easy preparation process, low cost, and good compatibility can avoid the potential immunogenicity and carcinogenicity of viral vectors 31 . Cationic liposome is an ideal nonviral vector; however, the cytotoxicity and low efficiency in vivo always restrict its application 32,33 .…”

Section: Introductionmentioning

confidence: 99%

Gasdermin E plasmid DNA/indocyanine green coloaded hybrid nanoparticles with spatiotemporal controllability to induce pyroptosis for colon cancer treatment

et al. 2023

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Pyroptosis is an immunogenic cell death and would trigger robust antitumor immunity. However, due to cytoxicity of pyroptosis executors, Gasdermin family proteins, it is indispensable to construct tumor-specific vectors. Here, we report the development of a novel vector named lipid-coated poly(lactideco-glycolide) (PLGA) nanoparticles coloaded with Gasdermin E expressing plasmid DNA (GSDME-pDNA) with a heat-inducible mouse heat shock protein 70 (mHSP70) as the promoter and a photosensitizer indocyanine green (ICG) to activate the mHSP70 element. The cellular internalization and transfection rate of the vector were remarkably enhanced by photothermal treatment. And the mHSP70 promoter further improved the gene transfection rate for about 15-fold. With the combination of oxaliplatin (OXA), the mechanism switch between apoptosis and pyroptosis was fulfilled by cleavage of GSDME through activated caspase-3, which promoted damage-associated molecular patterns (DAMPs) release and increased the infiltration of immune cells at the tumor site. This combination strategy not only prominently inhibited the growth of the treated tumor, but also exhibited a lethal effect on the distal tumors. Besides, mouse colon cancer cell CT26 overexpressing GSDME after OXA treatment had the potential to be the preventive tumor vaccine. This study provides a novel thought and feasible method for the clinical treatment of colon cancer.

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Disaccharide-polyethylenimine organic nanoparticles as non-toxic in vitro gene transporters and their anticancer potential

Cited by 4 publications

References 60 publications

Design, fabrication and evaluation of amphiphilic hyaluronic acid conjugates as efficient carriers of 6‐thioguanine for in vitro anticancer drug delivery applications

Design, fabrication and evaluation of amphiphilic hyaluronic acid conjugates as efficient carriers of 6‐thioguanine for in vitro anticancer drug delivery applications

Carbohydrate-Based Nanoparticles in Therapeutics and Biomedicine

Gasdermin E plasmid DNA/indocyanine green coloaded hybrid nanoparticles with spatiotemporal controllability to induce pyroptosis for colon cancer treatment

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