Disaccharidase Activity in Isolated Brush Border from the Gut of Ascaris suum

Gentner, Harry W.; Savage, William R.; Castro, Gilbert A.

doi:10.2307/3278082

Cited by 14 publications

(5 citation statements)

References 12 publications

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“…The presence of maltase, saccharase and trehalase in the homogenate of the gut of A. suum confirms the observation of various authors (van den Bossche et al, 1973;Gentner et al, 1972Gentner et al, , 1974. Moreover, in the present study a relatively high maltase activity was also observed in the homogenate of the uterus.…”

Section: Discussionsupporting

confidence: 93%

“…Activities of different disaccharidases have been demonstrated in the intestine of Ascaris lumbricoides (Carpenter, 1952;Fukushima, 1967;Palma et al, 1970) and Ascaris suum (Feist et al, 1965;Bossche et al, 1972;Gentner et al, 1972Gentner et al, , 1974. In the present study the activities of disaccharidases in different helminths of pigs parasitized in different parts of the gastro-intestinal tract have been determined.…”

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confidence: 61%

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Activity of disaccharidases in the intestinal contents of piglets infected with gastrointestinal nematodes and activity in these nematodes

Ënigk

Dey-Hazra

1978

J. Helminthol.

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The activity of maltase, saccharase, cellobiase, trehalase and lactase was estimated in the intestinal contents of non-infected pigs and pigs infected with several gastrointestinal nematodes and in the nematodes themselves.Enzyme activities in the intestinal contents of pigs infected with Hyostrongylus rubidus were similar to those in uninfected control pigs. However the enzyme activity of the contents of the whole intestine was increased in Strongyloides ransomi infections and moderately increased in Ascaris suum infection. In Trichuris and Oesophagostomum infections activity was only increased in the large intestine contents.Ascaris suum contained high activity of maltase and saccharase in its intestine and lower levels in other organs, while levels were much lower in other pig nematodes. Hyostrongylus rubidus and its third-stage larva did not contain any disaccharidases activity.The disaccharidases in the pig nematodes, especially in Strongyloides, were more sensitive to high temperatures (70°C) than those in the contents of the pig intestine.Some disaccharidases were demonstrable in embryonated Ascaris and Trichuris eggs and in third-stage larvae of Strongyloides and Oesophagostomum.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 61%

Activity of disaccharidases in the intestinal contents of piglets infected with gastrointestinal nematodes and activity in these nematodes

Ënigk

Dey-Hazra

1978

J. Helminthol.

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“…These facts suggest that nematodes utilize the glycosidases for intracellular digestion, whereas many trematodes may depend predominantly on the extracellular or intraluminal digestion through hydrolysis as indicated by HALTON (1967) and BOGITSH (1972). In A. suum, however, some other hydrolytic and proteolytic enzymes were demonstrated on or near the brush border (LEE, 1962 a, b;BORGERS et al, 1970;GENTNER et al, 1972;VAN DEN BOSSCHE & BORGERS, 1973).…”

Section: Discussionmentioning

confidence: 99%

Comparative histochemical studies of glycosidase activity in some helminths

Fujino

Ishii

1986

J. Helminthol.

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Comparative histochemical studies of glycosidase activity were carried out in Clonorchis sinensis, Eurytrema pancreaticum, Fasciola hepatica, Dipylidium caninum, Hymenolepis nana, Ascaris suum, Toxocara canis, Ancylostoma caninum, Trichuris vulpis and Dirofilaria immitis. The enzymes examined were: N-Acetyl-α-Glucosaminidase (Ec 3.2.1.30), α-Glucuronidase (Ec 3.2.1.31) and α-Galactosidase (Ec 3.2.1.23). There were variations in enzyme distribution and intensity among the species and also between trematodes and nematodes; no marked positive reaction for these enzymes occurred in cestodes. In some trematodes, the caeca, especially in the brush border, and the tegument, subtegumental cells and testes, were reactive to the enzymes. In nematodes, although there was variation in reactions among species, N-acetyl-α-glucosaminidase and α-galactosidase were localized in the hypodermis and lateral cords excluding the excretory canal, and coelomocytes, intestinal epithelium and the walls of the reproductive systems.

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“…According to Sajid et al, (1997) the aminopeptidases from the muscles of A. suum may play an important role in intracellular metabolism and inactivate neuropeptides. The presence of carbohydrates digesting enzymes in the intestine and in other parts of the body of A. suum has been detected by many authors (Palma et al, 1970;Gentner et al, 1972;Zenka & Prokopic 1984;Żółtowska, 1991, 2001Żółtowska & Dmitryjuk, 1998;Dmitryjuk & Żół-towska 2004). These authors determined the activity of α-amylase, maltase, invertase, palatinase and trehalase, but they did not find any activity of β-galactosidase (lactase) and β-glucosidase (celobiase).…”

Section: Discussionmentioning

confidence: 99%

“…According to Maki et al (1982) and Oue et al (2000) the activity of many enzymes in parasites' intestines depends on the composition of the absorbed food. The ability to digest numerous carbohydrates was observed in the intestine of A. suum (Gentner et al, 1972;Van den Bossche & Borgers, 1973;Żółtowska, 1991). The activity of endoproteases and lipase in the intestine of A. suum was determined by many authors, e.g.…”

Section: Introductionmentioning

confidence: 99%

The activity of selected hydrolases of adult female Ascaris suum, Goeze 1782

Dziekońska-Rynko

2006

Helminthologia

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59 SummaryThe activity of hydrolases was measured in the perienteric fluid, muscles and three sections of the intestine of female Ascaris suum. In none of the examined samples was the activity of lipase and α-galactosidase detected. The activity of hydrolases in anterior, middle and posterior sections of the A. suum intestine was similar. The high activity of the following glucosidases: β-galactosidase (lactase), N-acetyl-β glucosaminidase and α-fucosidase, was detected in the contents and extracts from the intestinal wall, apart from the activity of those found in earlier studies. In perienteric fluid and in extracts from muscles, a high activity of β-glucuronidase and β-glucosidase was detected; such activity was not found in the intestine.

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Disaccharidase Activity in Isolated Brush Border from the Gut of Ascaris suum

Cited by 14 publications

References 12 publications

Activity of disaccharidases in the intestinal contents of piglets infected with gastrointestinal nematodes and activity in these nematodes

Activity of disaccharidases in the intestinal contents of piglets infected with gastrointestinal nematodes and activity in these nematodes

Comparative histochemical studies of glycosidase activity in some helminths

The activity of selected hydrolases of adult female Ascaris suum, Goeze 1782

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