Directional porosity of porcine small‐intestinal submucosa

Ferrand, B.; Kokini, Klod; Badylak, Stephen F.; Geddes, L. A.; Hiles, Michael C.; Morff, R. J.

doi:10.1002/jbm.820271004

Cited by 36 publications

(25 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…decrease from the serosal (abluminal) surface to mucosal (luminal) surface of the ECM. 11 Additionally, interspersed throughout the collagen network of SIS are remnant structures of blood vessels (tissue tags) and an occasional thick lymphoid tissue structure (Peyer's patch). AllodermÒ consists of 2 layers, papillary dermis and reticular dermis, that differ in porosity.…”

Section: Discussionmentioning

confidence: 99%

Oxygen Diffusion through Natural Extracellular Matrices: Implications for Estimating “Critical Thickness” Values in Tendon Tissue Engineering

Androjna

Gatica

Belovich

et al. 2008

Tissue Engineering Part A

View full text Add to dashboard Cite

Oxygen is necessary for maintaining cell proliferation and viability and extracellular matrix (ECM) production in 3-dimensional tissue engineering. Typically, diffusion is the primary mode for oxygen transport in vitro; thus, ensuring an adequate oxygen supply is essential. In this study, we determined the oxygen diffusion coefficients of 3 natural ECMs that are being investigated as construct scaffolds for tendon tissue engineering: small-intestine submucosa (SIS), human dermis (AllodermÒ), and canine fascia lata. Diffusion coefficients were determined using a standard diffusion cell system. The ranges for each matrix type were: SIS: 7Â10 À6 À 2Â10 À5 cm 2 /s, AllodermÒ: 1.9 À 3.1Â10 À5 cm 2

show abstract

Section: Discussionmentioning

confidence: 99%

Oxygen Diffusion through Natural Extracellular Matrices: Implications for Estimating “Critical Thickness” Values in Tendon Tissue Engineering

Androjna

Gatica

Belovich

et al. 2008

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…For SIS disks, chondrocytes were seeded onto the ablumenal surface, which demonstrated a greater degree of porosity. 12 The resulting cell count was approximately 2 3 10 7 cells per disk. No further medium was added at this time in order to allow chondrocyte attachment.…”

Section: Scaffold Inoculation With Chondrocytes and Implantation Intomentioning

confidence: 99%

Small Intestinal Submucosa versus Salt-Extracted Polyglycolic Acid-Poly-L-lactic Acid: A Comparison of Neocartilage Formed in Two Scaffold Materials

et al. 2002

View full text Add to dashboard Cite

This study sought to compare differences in neocartilage produced over time from two types of resorbable scaffold materials. One material was entirely synthetic and contained a polyglycolic acid-poly-L-lactic acid matrix (PGA-PLLA). The second scaffold material was bioactive and consisted of a four-layered construct of porcine small intestinal submucosa (SIS). Disk-shaped scaffolds were seeded with canine chondrocytes and implanted into athymic mice for periods of 5, 8, 12, and 24 weeks. Constructs were examined microscopically, assayed for hydroxyproline (HP) and glycosaminoglycan (GAG) content, and collagen typed (I or II) at each time period. Creep indentation tests determined aggregate and shear modulus, permeability, and thickness. Results indicated that SIS maintained its thickness through the first 12 weeks, and then doubled by week 24. The 24-week tissue appeared chondroid-like and possessed high GAG content. Tissues derived from PGA-PLLA scaffolds were lower in HP content than SIS-derived tissues, but type II collagen was demonstrated only in PGA-PLLA-derived tissues at 24 weeks. Mechanical properties were not significantly different for any tissue over time (p > 0.05), but aggregate and shear modulus mean values were consistently higher for PGA-PLLA-derived tissues at nearly every time interval. This, coupled with the presence of collagen types I and II, suggested a more congruent solid phase may be forming within the extracellular matrix of tissues derived from PGA-PLLA scaffolds. Future study is necessary to compare these materials under simulated loading conditions.

show abstract

“…10,11 In these studies the porosity was correlated with applied pressure and uniaxial tension. In a bladder context, and in normal conditions, the urinary bladder is not normally subjected to a pressure above 10 cm of water.…”

Section: Discussionmentioning

confidence: 99%

Porosity of porcine bladder acellular matrix: Impact of ACM thickness

Farhat

Chen

Erdeljan

et al. 2003

J Biomedical Materials Res

View full text Add to dashboard Cite

The objectives of this study are to examine the porosity of bladder acellular matrix (ACM) using deionized (DI) water as the model fluid and dextran as the indicator macromolecule, and to correlate the porosity to the ACM thickness. Porcine urinary bladders from pigs weighing 20-50 kg were sequentially extracted in detergent containing solutions, and to modify the ACM thickness, stretched bladders were acellularized in the same manner. Luminal and abluminal ACM specimens were subjected to fixed static DI water pressure (10 cm); and water passing through the specimens was collected at specific time interval. While for the macromolecule porosity testing, the diffusion rate and direction of 10,000 MW fluoroescein-labeled dextrans across the ACM specimens mounted in Ussing's chambers were measured. Both experiments were repeated on the thin stretched ACM. In both ACM types, the fluid porosity in both directions did not decrease with increased test duration (3 h); in addition, the abluminal surface was more porous to fluid than the luminal surface. On the other hand, when comparing thin to thick ACM, the porosity in either direction was higher in the thick ACM. Macromolecule porosity, as measured by absorbance, was higher for the abluminal thick ACM than the luminal side, but this characteristic was reversed in the thin ACM. Comparing thin to thick ACM, the luminal side in the thin ACM was more porous to dextran than in the thick ACM, but this characteristic was reversed for the abluminal side. The porcine bladder ACM possesses directional porosity and acellularizing stretched urinary bladders may increase structural density and alter fluid and macromolecule porosity.

show abstract

Directional porosity of porcine small‐intestinal submucosa

Cited by 36 publications

References 4 publications

Oxygen Diffusion through Natural Extracellular Matrices: Implications for Estimating “Critical Thickness” Values in Tendon Tissue Engineering

Oxygen Diffusion through Natural Extracellular Matrices: Implications for Estimating “Critical Thickness” Values in Tendon Tissue Engineering

Small Intestinal Submucosa versus Salt-Extracted Polyglycolic Acid-Poly-L-lactic Acid: A Comparison of Neocartilage Formed in Two Scaffold Materials

Porosity of porcine bladder acellular matrix: Impact of ACM thickness

Contact Info

Product

Resources

About