Directional Photonic Wire Mediated by Homo-Förster Resonance Energy Transfer on a DNA Origami Platform

Nicoli, Francesca; Barth, Anders; Bae, Wooli; Neukirchinger, Fabian; Crevenna, Álvaro H.; Lamb, Don C.; Liedl, Tim

doi:10.1021/acsnano.7b05631

Cited by 69 publications

(67 citation statements)

References 54 publications

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“…DNA has been widely used as a platform to prepare chromophoric arrays [7][8][9][10][11] , and recently, photonic arrays and circuits based on DNA nano-structures have been reported [12][13][14][15] . In these structures, EM and hetero FRET play crucial roles in efficient energy harvesting and transport [16][17][18][19][20][21][22][23][24][25][26] . Several research groups, including ours, have utilized DNA scaffolds to quantify orientation and distance dependence of hetero FRET from a donor to an acceptor [27][28][29][30][31][32][33] .…”

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confidence: 99%

Quantitative evaluation of energy migration between identical chromophores enabled by breaking symmetry

et al. 2018

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Energy migration between the identical chromophores is a necessary process in both natural and artificial photosynthesis. The distance and orientation dependence of energy migration have not been experimentally investigated in detail. Here we propose a method to investigate energy migration. Two fluorophores are introduced into one strand of a DNA duplex with a quencher placed opposite one of fluorophores. This design enables asymmetrization of identical fluorophores and allows one fluorophore to behave as an acceptor. The emission intensities and lifetimes decrease depending on the efficiency of energy migration. Distance and orientation dependence are successfully quantified, and the excitation energy migration efficiencies measured are in excellent agreement with those calculated based on Förster theory. We also demonstrate that multi-step energy migration among four fluorophores can be estimated from the theory. These results may provide a basis for design and preparation of efficient light-harvesting photonic devices and chemical probes.

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confidence: 99%

Quantitative evaluation of energy migration between identical chromophores enabled by breaking symmetry

et al. 2018

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“…Work realized by the Hanley group used DNA templates to align fluorescent proteins (monomeric teal fluorescent protein or labeled bovine serum albumin) to also look at HomoFRET transfer, finding that the total fluorescence was not the sum of the individual components and confirming the existence of parasitic traps . Liedl and collaborators looked at HomoFRET within the context of a DNA origami that displayed non‐linearly arranged HomoFRET regions (see Figure D) . Their relay was more triangular shaped, but still showed efficient relay capabilities with the HomoFRET section increasing transfer from 0% to 5% over 16 nm.…”

Section: Fluorescently Labeled Dna Materialsmentioning

confidence: 99%

Section: Fluorescently Labeled Dna Materialsmentioning

confidence: 99%

Utilizing the Organizational Power of DNA Scaffolds for New Nanophotonic Applications

Bui

Dı́az

Fontana

et al. 2019

Advanced Optical Materials

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Rapid development of DNA technology has provided a feasible route to creating nanoscale materials. DNA acts as a self‐assembled nanoscaffold capable of assuming any three‐dimensional shape. The ability to integrate dyes and new optical materials such as quantum dots and plasmonic nanoparticles precisely onto these architectures provides new ways to exploit their near‐ and far‐field interactions. A fundamental understanding of these optical processes will help drive development of next‐generation photonic nanomaterials. This review is focused on latest progress in DNA‐based photonic materials and highlights DNA scaffolds for rapidly assembling and prototyping nanoscale optical devices. Three areas are discussed including intrinsically active DNA structures displaying chiral properties, DNA scaffolds hosting plasmonic nanomaterials, and fluorophore‐labeled DNAs that engage in Förster resonance energy transfer and give rise to complex molecular photonic wires. An explanation of what is desired from these optical processes when harnessed sets the tone for what DNA scaffolds are providing toward each focus. Examples from the literature illustrate current progress along with a discussion of challenges to overcome for further improvements. Opportunities to integrate diverse classes of optically active molecules including light‐generating enzymes, fluorescent proteins, nanoclusters, and metal–chelates in new structural combinations on DNA scaffolds are also highlighted.

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“…Fluorophores are often directly incorporated into the DNA “staple” strands for arrangement on DNA origami structures. 27 − 32 …”

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confidence: 99%

DNA Origami Route for Nanophotonics

et al. 2018

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The specificity and simplicity of the Watson–Crick base pair interactions make DNA one of the most versatile construction materials for creating nanoscale structures and devices. Among several DNA-based approaches, the DNA origami technique excels in programmable self-assembly of complex, arbitrary shaped structures with dimensions of hundreds of nanometers. Importantly, DNA origami can be used as templates for assembly of functional nanoscale components into three-dimensional structures with high precision and controlled stoichiometry. This is often beyond the reach of other nanofabrication techniques. In this Perspective, we highlight the capability of the DNA origami technique for realization of novel nanophotonic systems. First, we introduce the basic principles of designing and fabrication of DNA origami structures. Subsequently, we review recent advances of the DNA origami applications in nanoplasmonics, single-molecule and super-resolution fluorescent imaging, as well as hybrid photonic systems. We conclude by outlining the future prospects of the DNA origami technique for advanced nanophotonic systems with tailored functionalities.

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Directional Photonic Wire Mediated by Homo-Förster Resonance Energy Transfer on a DNA Origami Platform

Cited by 69 publications

References 54 publications

Quantitative evaluation of energy migration between identical chromophores enabled by breaking symmetry

Quantitative evaluation of energy migration between identical chromophores enabled by breaking symmetry

Utilizing the Organizational Power of DNA Scaffolds for New Nanophotonic Applications

DNA Origami Route for Nanophotonics

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