Directional evolution of TetR protein and development of a fluoroimmunoassay for screening of tetracyclines in egg

Wang, Ge; Xia, Wan Qiu; Liu, Juxiang; Wang, Jian Ping; Liu, Jing

doi:10.1016/j.microc.2019.104184

Cited by 17 publications

(10 citation statements)

References 36 publications

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“…By now, there have been some papers reporting the use of the receptor as a recognition reagent for detection of some analytes, 11,12 including β-agonists, 13 β-lactams, 14,15 and tetracyclines. 16,17 Results show that their specificities and recognition spectra are comparable to or better than the controlled antibodies.…”

Section: ■ Introductionmentioning

confidence: 94%

“…In comparison, the receptor is the natural macromolecular protein, and it is usually the active site of one class of drugs, so a specific receptor can recognize all the species of this class of drugs. By now, there have been some papers reporting the use of the receptor as a recognition reagent for detection of some analytes, , including β-agonists, β-lactams, , and tetracyclines. , Results show that their specificities and recognition spectra are comparable to or better than the controlled antibodies.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism

Liu

Wang

2021

J. Agric. Food Chem.

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In this study, the dihydropteroate synthase of Staphylococcus aureus was obtained, and its recognition mechanisms for 31 sulfonamide drugs were studied. Results showed that their core structure matched well with the binding pocket of paraaminobenzoic acid, and all the sulfonamide side chains were out of the binding pocket. Hydrogen bonds and hydrophobic interactions were the main intermolecular forces, and the key amino acids were Gly171 and Lys203. The binding sites in sulfonamide molecules were mainly around the para-aminobenzenesulfonamide part. This enzyme was used to develop a fluorescence polarization assay for detection of these drugs in chicken muscles. The change trends of half of inhibition concentrations and crossreactivities for the 31 drugs were identical with the receptor−ligand affinities. The limits of detection were in the range of 2.0−38.5 ng/g, and one assay could be finished within several minutes. Therefore, this method could be used for multiscreening of sulfonamide residues in meat samples.

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Section: ■ Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism

Liu

Wang

2021

J. Agric. Food Chem.

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“…Immunoassays of various analytes in eggs are often preceded by a rather laborious sample preparation. So, for example, for analysis of tetracyclines (Wang, Xia, Liu, Wang, & Liu, 2019), six coccidiostatics (Bienenmann-Ploum et al, 2012) and antibiotics from amphenicol group (Lei et al, 2018), the sample preparation included extraction with acetonitrile, removing of the precipitated ingredients by centrifugation, separation of the organic supernatant and its complete evaporation, re-dissolution of the precipitate in a buffer with hexane or methanol, additional centrifugation to obtain a supernatant for testing in immunoassay.…”

Section: Examination Of Matrix Effectmentioning

confidence: 99%

Development of indirect competitive enzyme-linked immunoassay of colistin for milk and egg analysis

Galvidis

Eremin

Burkin

2020

Food and Agricultural Immunology

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Polyclonal antibodies were obtained by immunizing rabbits with polymyxin B (PMB) conjugated to bovine serum albumin. Based on the heterologous coating conjugate of PMB with periodateoxidized gelatin and the resulting antibodies, an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed to detect residual colistin (COL), a structural analogue of PMB in livestock products. The developed analysis was characterized by close specificity towards PMB (100%) and COL (88%), sensitivity (IC 50 = 5.7 ng/mL), dynamic range (IC 20 -IC 80 = 1.0-80 ng/mL) and the detection limit of COL (IC 10 = 0.4 ng/mL). It was possible to quantify the antibiotic in milk and eggs without special and labour-intensive sample pretreatment using a simple 10-and 50-fold dilution of the corresponding samples. The developed analysis system made it possible to record the maximum residue limit of COL in dairy products (50 μg/kg) and eggs (300 μg/kg) with 89-104% recovery of analyte.

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“…It is well known that the receptor is a natural macromolecule, and a specific receptor is the usual target site of one class of drugs, so in theory, it should recognize all the species of this class of drugs. During the past few years, some receptors have been used as recognition reagents to develop pseudo-immunoassays for the detection of sulfonamides [ 19 , 20 , 21 , 22 , 23 ], β-lactams [ 24 ], tetracyclines [ 25 , 26 , 27 ], and β 2 -agonists [ 28 ]. Results showed that these receptors exhibited broader detection spectra than the controlled antibodies.…”

Section: Introductionmentioning

confidence: 99%

Development of a Fluorescence Polarization Assay for Multi-Determination of 10 Aminoglycosides in Pork Muscle Sample Based on Ribosomal Protein S12 and Studying Its Recognition Mechanism

Xia

Zhang

Wang

2022

Foods

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The residues of aminoglycosides in foods of animal origin are a potential risk to consumers. There have been some immunoassays reported for the screening of aminoglycoside residues, but the method showing the broadest detection spectrum can only be used to detect two drugs. This is because a broad specific recognition reagent is not available. In the present study, the receptor of aminoglycosides (ribosomal protein S12 of Lysinibacillus sphaericus) was expressed, and its affinities and recognition mechanisms for 10 aminoglycosides were studied by using surface plasmon resonance and molecular docking, respectively. Then the receptor was used as a recognition reagent to develop a fluorescence polarization assay on a 96-well microplate for the detection of the 10 drugs in pork muscle samples. The limits of detection for the 10 drugs ranged from 5.25 to 30.25 ng/g. The sensitivities for the 10 drugs were generally consistent with their respective receptor affinities and binding energies. After comprehensive comparison, the method performances were better than all the previously reported immunoassays for aminoglycosides. This is the first study reporting the recognition mechanisms of ribosomal protein S12 of Lysinibacillus sphaericus for 10 aminoglycosides and the use of it as a recognition reagent to develop a pseudo-immunoassay for the multi-determination of aminoglycosides in food samples.

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Directional evolution of TetR protein and development of a fluoroimmunoassay for screening of tetracyclines in egg

Cited by 17 publications

References 36 publications

Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism

Development of a Dihydropteroate Synthase-Based Fluorescence Polarization Assay for Detection of Sulfonamides and Studying Its Recognition Mechanism

Development of indirect competitive enzyme-linked immunoassay of colistin for milk and egg analysis

Development of a Fluorescence Polarization Assay for Multi-Determination of 10 Aminoglycosides in Pork Muscle Sample Based on Ribosomal Protein S12 and Studying Its Recognition Mechanism

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