Directional Correlation Studies of Anomalous Water in Carbonic Anhydrase

Time differential perturbed angular correlation (TDPAC) studies of the 133Ba ion uptake in bone crystals

“…In figure 4 ( a ) the time-independent anisotropy for 133Cs in the BaClz aqueous solution shows an unperturbed angular correlation, as expected, because of the Brownian motion of the 133Ba ions in the solution. Taking into account the solid-angle correction factors, the measured angular correlation coefficient is A 2 = +0.046 f 0.005, in good agreement with previous measurements (Bodenstedt eta1 1959, Glass andGraf 1970). For the polycrystalline '33BaC12(2Hz0) source the anisotropy curve shows a time dependence (figure 4(b)).…”

“…The technique of perturbed angular correlation (PAC) has been used successfully for some years for obtaining information on the motion and structure of biological molecules (Leipert et a1 1968, Shirley 1970, Glass and Graf 1970, Meares et a1 1972, Bauer et a1 1974. In a recent paper, Graf et af (1974) reviewed the applications of PAC in the biological field, with special reference to the study of protein-metal interactions, most study to date having centred on measurements of molecular rotational correlation times and internal electric field gradients in metallo-proteins (Meares et a1 1969, Bauer and Limkilde 1974, Richer et a1 1974.…”

Statistical Time Events in Enzymes: A Physical Assessmen

“…More exactly, it can be shown 40 that the light beam interacts with the oscillator fluctuations of the medium, and that the Raman intensities can be directly calculated by applying the fluctuation-dissipation theorem according to the response function theory. In other words, the Raman scattering spectrum can be considered as the spectrum of the spontaneous fluctuations of the medium at optical frequencies which must be determined by measurement performed on the associate radiation field.…”

Perturbed angular correlation spectroscopy and its application to metal sites in proteins: possibilities and limitations

“…These investigations demonstrated that spectral information could be obtained by PAC spectroscopy. Additional PAC work on carbonic anhydrase was performed in 1974 (Glass & Graf, 1970;Bauer et al 1974). In 1964 it was shown that in this…”