Directed Sequencing of Plant Specific DNA Identifies the Dietary History of Four Species of Auchenorrhyncha (Hemiptera)

Cooper, William C.; Marshall, Adrian T.; Foutz, Jillian; Wildung, Mark R.; Northfield, Tobin D.; Crowder, David W.; Leach, Heather; Leskey, Tracy; Halbert, Susan E.; Snyder, James

doi:10.1093/aesa/saab053

Cited by 13 publications

(13 citation statements)

References 48 publications

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“…Our results support previous findings of polyphagous feeding behavior of early instars of L. delicatula [ 1 , 14 , 35 ]. Particularly, many authors reported the narrowing pattern of L. delicatula host plant use, from the first instars to adults [ 1 , 3 , 4 , 35 , 36 ].…”

Section: Discussionsupporting

confidence: 93%

“…We specifically used this published list to determine whether our findings could potentially contribute to and expand the host plant list of L. delicatula . Thirteen novel plant taxa that we reported in this study were also determined by comparison with the findings by Cooper et al [ 14 ], the only study that used DNA metabarcoding of gut contents of the first instars of L. delicatula . We emphasize that these are novel food plants, and whether they support the development and reproduction of L. delicatula could also be a focus for future studies.…”

Section: Discussionmentioning

confidence: 85%

“…The presence of all of these identified ingested plant species in Cecil County, Maryland, USA was confirmed through the USDA PLANT database ( , accessed on 3 April 2022). Finally, when the list of identified ingested plants was compared with the latest published host plant list for L. delicatula [ 9 ], and with the recent findings on ingested plants from L. delicatula gut contents [ 14 ], 13 novel species/genera were identified ( Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…Our findings also support, once again, the preference for tree of heaven, Ailanthus altissima , which was present at the collection site and was detected in 55% of all the samples. Cooper et al [ 14 ] showed that up to 17 plant families can be detected from a combined 1st–3rd instars; of these, however, only 10 different taxa (belonging to 7 plant families) were reported for the first instars with 2.5 plant taxa per nymphal individual. The authors, however, used only three nymphal individuals and amplified portions of trn F and ITS genes.…”

Section: Discussionmentioning

confidence: 99%

“…Due to their small size, cryptic coloration, and a discreet sap-feeding behavior, the first instars are challenging to find and monitor. While most studies on L. delicatula feeding preferences still focus on the experimental observations [ 5 , 13 ], recent advances using PCR-based DNA analyses provide opportunities for rapid and reliable detection and identification of L. delicatula ’s diet [ 6 , 14 ]. In our previous work on plant DNA detection from the gut contents of L. delicatula [ 6 ], we focused on late nymphal instars only (third and fourth nymphal instars).…”

Section: Introductionmentioning

confidence: 99%

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Diverse Host Plants of the First Instars of the Invasive Lycorma delicatula: Insights from eDNA Metabarcoding

McPherson

Avanesyan

Lamp

2022

Insects

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Identification of host plants of the invasive spotted lanternfly, Lycorma delicatula (Hemiptera: Fulgoridae), has been the focus of many studies. While the adults and late nymphs are relatively easy to observe on plants and to use for molecular gut-content analysis, studying the early instars is more challenging. This study is the continuation of our ongoing efforts to determine the host range for each developmental stage of L. delicatula. In the present study, we focused exclusively on the first nymphal instars, and we used a novel approach, utilizing “bulk” DNA extracts for DNA metabarcoding of nymphal gut contents, to identify all the detectable plants that the nymphs had ingested prior to being collected. We were able to obtain high-quality amplicons (up to 406 bp) of a portion of the rbcL gene and detect 27 unique ingested plant species belonging to 17 families. Both native and introduced plants with the prevalence of trees and grasses were present among the ingested plants. We also identified 13 novel host plants that have not been previously reported for L. delicatula on the U.S. territory. The results from our study have important applications for developing effective programs on early monitoring of invasive L. delicatula.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Diverse Host Plants of the First Instars of the Invasive Lycorma delicatula: Insights from eDNA Metabarcoding

McPherson

Avanesyan

Lamp

2022

Insects

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What's on the menu? A novel molecular gut content analysis to investigate the feeding behavior of phytophagous insects

Fluch,

Chignola,

Corretto

et al. 2024

Ecology and Evolution

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The relationship between phytophagous insects and plants is a central aspect of food webs and ecosystem functioning. The introduction of new species into an environment can have significant impacts on the food web of a native ecosystem. In many cases, there is a lack of knowledge on the biology and feeding behavior of invasive species prior their introduction and in the invaded regions. Gut content analyses of insects have provided valuable information on the host spectrum of insects. However, current approaches are time‐consuming and costly. Here, we describe a new molecular gut content analysis (GCA) approach using the Oxford Nanopore (ONT) Flongle sequencing platform to characterize the plant DNA present in the gut of the highly polyphagous insect species Halyomorpha halys. We demonstrate that this technique efficiently amplifies and correctly identifies plant DNA in a mock community. We performed a feeding experiment to determine the sensitivity of this approach and to assess how long the plant DNA can be detected. All plants used in the feeding experiment were correctly identified and detected after 56 days. Surprisingly, we also detected various plant genera that were not included in the feeding experiment and thus were likely ingested months before the experiment. Our study suggests that the GCA using the ONT Flongle sequencing platform represents a rapid and cost‐efficient diagnosis of the dietary preferences, host range, and the diversity of consumed plant species of pest insects with high precision.

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Prolonged phloem feeding by the spotted lanternfly, an invasive planthopper, alters resource allocation and inhibits gas exchange in grapevines

et al. 2022

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Spotted lanternfly ( Lycorma delicatula White; SLF) is a phloem‐feeding planthopper invasive to the Eastern United States that can feed on a range of wild and cultivated plant species. Since its 2014 introduction in the United States, large infestations and subsequent economic damage have been reported in cultivated grapevines, but no studies have detailed grapevine physiological responses to SLF phloem feeding. This study investigated grapevine‐SLF interactions, detailing how different infestation densities affect leaf gas exchange and end‐season concentrations of nonstructural carbohydrates and nitrogen in vegetative and perennial tissues of two Vitis species. Effects on fruit ripeness parameters and dormant bud freeze tolerance were examined, in addition to other year‐after effects. Phloem feeding by low densities (≤4 SLF shoot −1 ) had minimal effects, whereas greater densities (5–15 SLF shoot −1 ) increasingly affected carbohydrate and nitrogen dynamics in both Vitis species. Phloem feeding substantially affected starch and, to a lesser extent, total nitrogen concentrations of woody roots. Prolonged exposure strongly reduced leaf gas exchange. We conclude that intensive late‐season phloem feeding by large adult SLF population densities (≥8 SLF shoot −1 ) can induce carbon limitation, with the potential for negative year‐after effects in cases of severe belowground carbon depletion. This work presents novel insights into SLF‐grapevine interactions, identifies avenues of future SLF‐plant research, and assists the development of action thresholds for SLF management in vineyards.

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Directed Sequencing of Plant Specific DNA Identifies the Dietary History of Four Species of Auchenorrhyncha (Hemiptera)

Cited by 13 publications

References 48 publications

Diverse Host Plants of the First Instars of the Invasive Lycorma delicatula: Insights from eDNA Metabarcoding

Diverse Host Plants of the First Instars of the Invasive Lycorma delicatula: Insights from eDNA Metabarcoding

What's on the menu? A novel molecular gut content analysis to investigate the feeding behavior of phytophagous insects

Prolonged phloem feeding by the spotted lanternfly, an invasive planthopper, alters resource allocation and inhibits gas exchange in grapevines

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