Directed self-assembly of proteins into discrete radial patterns

Thakur, Garima; Prashanthi, K.; Thundat, Thomas

doi:10.1038/srep01923

Cited by 19 publications

(15 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regulating biomolecular immobilization is essential for chemical modification of biochips and for biosensing of surfaces for protein capture . We used thin film gold surface implanted on glass, which had 192 spots available as the parent substrate due to their stability and chemical assembly features through Au‐S bonds in biochips (supplied by Thermohybaid, Interactiva Division, Ulm, Germany) …”

Section: Methodsmentioning

confidence: 99%

S‐S‐PEG‐COOH Self‐Assembled Monolayer on Gold Surface Enabled a Combined Assay for Serological EBV Antibody Isotypes

Liu

et al. 2018

Proteomics Clinical Apps

View full text Add to dashboard Cite

Purpose: Epstein-Barr virus (EBV) is a ubiquitous human gamma herpes virus that infects human epithelial cells and B lymphocytes. It would be potentially valuable to develop novel combined assays to benefit screening for large panels of samples of EBV infectious diseases. Experimental design: A simple antigen-probed biochip that is modified with S-S-PEG-COOH and is used as a label-free high-throughput screening method for a combined detection of EBV capsid antigen IgM antibody, capsid antigen IgG antibody, and nuclear antigen IgG antibody.Results: This protein biochip has similar feasibility, sensitivity, and specificity in comparison with Liaison chemiluminescent immunoassay (CLIA). Detection limit of the EBV antibodies by the biochip is almost identical to that by CLIA-L (2.91 U mL -1 vs 3.00 U mL -1 for EBNA-1 IgG, 8 U mL -1 vs10 U mL -1 for EBV-VCA IgG, and 3.5 U mL -1 vs 10 U mL -1 for EBV-VCA IgM). Tests of the three serological antibodies against EBV by the biochip are consistent with the CLIA-L method in 274 clinical sera, respectively. Finally, the combined biochip is successfully utilized for diagnostic identification of EBV infection in 14 patients with infectious mononucleosis (IM) and 25 patients with systemic lupus erythematosus SLE, as well as additional 10 known real-time PCR positive patients.

show abstract

Section: Methodsmentioning

confidence: 99%

S‐S‐PEG‐COOH Self‐Assembled Monolayer on Gold Surface Enabled a Combined Assay for Serological EBV Antibody Isotypes

Liu

et al. 2018

Proteomics Clinical Apps

View full text Add to dashboard Cite

show abstract

“…Based on microfabrication and self-assembled monolayer (SAM) technologies1516, we describe a method to selectively immobilize kinesin and dynein in two different regions. After evaluation of the selective coating, we study MT behavior at the boundaries and categorize these MTs into one of four groups based on their behaviors.…”

mentioning

confidence: 99%

Tug-of-war of microtubule filaments at the boundary of a kinesin- and dynein-patterned surface

Ikuta

Kamisetty

Shintaku

et al. 2014

Sci Rep

View full text Add to dashboard Cite

Intracellular cargo is transported by multiple motor proteins. Because of the force balance of motors with mixed polarities, cargo moves bidirectionally to achieve biological functions. Here, we propose a microtubule gliding assay for a tug-of-war study of kinesin and dynein. A boundary of the two motor groups is created by photolithographically patterning gold to selectively attach kinesin to the glass and dynein to the gold surface using a self-assembled monolayer. The relationship between the ratio of two antagonistic motor numbers and the velocity is derived from a force-velocity relationship for each motor to calculate the detachment force and motor backward velocity. Although the tug-of-war involves >100 motors, values are calculated for a single molecule and reflect the collective dynein and non-collective kinesin functions when they work as a team. This assay would be useful for detailed in vitro analysis of intracellular motility, e.g., mitosis, where a large number of motors with mixed polarities are involved.

show abstract

“…However, for scaffold or substrate based protein pattern designing there are various approaches that are being used such as layer by layer strategy [13], use of protein templates [14], Electrostatic interaction induced assemblies [15], ligand or linker -based protein assemblies [16], gradient-directed assemblies [17] and Langmuir-Blodgett films [18].…”

Section: Two Dimensional Protein (2-d) Self-assembliesmentioning

confidence: 99%

Designing Protein Self-Assembly for Smart Materials

Thakur

Thundat

2017

JNMR

Self Cite

View full text Add to dashboard Cite

Protein self-assembly is advancing in this century as an extraordinary research field. Spontaneous assemblies of proteins or peptides into highly ordered nanoor-microstructures offer unprecedented advances in biotechnology. Designing protein self-assemblies is a challenging field but has huge potential to impact the biosensing industry. In this article we will focus on the most recent chemical and spontaneous self-assemblies of proteins resulting in pattern formation.

show abstract

Directed self-assembly of proteins into discrete radial patterns

Cited by 19 publications

References 44 publications

S‐S‐PEG‐COOH Self‐Assembled Monolayer on Gold Surface Enabled a Combined Assay for Serological EBV Antibody Isotypes

S‐S‐PEG‐COOH Self‐Assembled Monolayer on Gold Surface Enabled a Combined Assay for Serological EBV Antibody Isotypes

Tug-of-war of microtubule filaments at the boundary of a kinesin- and dynein-patterned surface

Designing Protein Self-Assembly for Smart Materials

Contact Info

Product

Resources

About