Directed evolution of Thermomyces lanuginosus lipase to enhance methanol tolerance for efficient production of biodiesel from waste grease

Tian, Kaiyuan; Tai, Kee; Chua, Bryan Jian Wei; Li, Zhi

doi:10.1016/j.biortech.2017.05.108

Cited by 69 publications

(34 citation statements)

References 44 publications

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“…Food sources are essential feedstock for first-generation biofuels and can also contribute to the competition of food [3]. While only 5% of the world's arable land is used to produce renewable resources in the form of first-generation biofuels, it has made a substantial impact on higher food and feed prices [4]. However, the existing effects of biofuels on the increase in food price remain uncertain in research or news reports.…”

Section: Introductionmentioning

confidence: 99%

Biodiesel and green diesel generation: an overview

Vignesh¹,

Kumar

Ganesh

et al. 2021

Oil Gas Sci. Technol. – Rev. IFP Energies nouvelles

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First, second, third, and fourth-generation biofuels are continuously evolving as a promising substitute to petrodiesel catalyzed by energy depletion, economic and environmental considerations. Bio-diesel can be synthesized from various biomass sources, which are commonly divided into FAME and renewable biodiesel. FAME biodiesel is generally produced by the transesterification of vegetable oils and fats while renewable diesel is produced by hydro-deoxygenation of vegetable and waste oils and fats. The different generation, processing technologies and standards for FAME and renewable biodiesel are reviewed. Finally, the life cycle analysis and production cost of conventional and renewable biodiesel are described.

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Section: Introductionmentioning

confidence: 99%

Biodiesel and green diesel generation: an overview

Vignesh¹,

Kumar

Ganesh

et al. 2021

Oil Gas Sci. Technol. – Rev. IFP Energies nouvelles

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“…Methanol and edible waste oil were also converted into biodiesel and glycerin via the actions of microbial lipase ( Karmee et al, 2015 ). Such as, T. lanuginosus lipase form catalyzed methanolysis of waste oil for the production of biodiesel ( Tian et al, 2017 ). Using the commercial lipase Novozyme ® 435 (Made by Novozymes), edible waste oil was used as raw material to produce biodiesel via enzyme-catalyzed regeneration ( Sorte et al, 2020 ).…”

Section: Heterologous Expression and Molecular Modification Of Microbial Lipasesmentioning

confidence: 99%

“…Similarly, lipase obtained from staphylococcal strains has been used in the manufacture of antioxidants such as ethanol-acetate and eugenyl benzoate (Horchani et al, 2012). Dai and Xia, 2005;Herrera-Lopez, 2012;Horchani et al, 2012;Bancerz, 2017;Kumar et al, 2017;Memarpoor-Yazdi et al, 2018 Pulp and paper Hydrolysis Paper with improved quality Demuner et al, 2011;Horchani et al, 2012;Liu et al, 2012;Almeida et al, 2018;Patel et al, 2019 Washing Hydrolysis Removal of fats Niyonzima and More, 2015;Phuah et al, 2015;Kumar et al, 2016;Unni et al, 2016;Holland and Sajic, 2017;Mehta et al, 2017;Saraswat et al, 2017;Abol-Fotouh et al, 2021 Biodiesel Transesterification, hydrolysis, synthesis Biodiesel production Karmee et al, 2015;Tran et al, 2016;Khosla et al, 2017;Tian et al, 2017;Li et al, 2020;Sorte et al, 2020;Talavari et al, 2020 Leather Hydrolysis Leather products Kavitha et al, 2018;Rashid et al, 2018 Cosmetics Synthesis Emulsifiers, moisturizers Gupta et al, 2015;Kim et al, 2015;Ugo et al, 2017;Uppada et al, 2017;Tufiño et al, 2019 Environmental protection Transesterification, hydrolysis Environmental detection and repair Lauprasert et al, 2017;Bucur et al, 2018;Hassan et al, 2018;…”

Section: Medical Applications Of Microbial Lipasesmentioning

confidence: 99%

A Valuable Product of Microbial Cell Factories: Microbial Lipase

Yao

Liu

et al. 2021

Front. Microbiol.

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As a powerful factory, microbial cells produce a variety of enzymes, such as lipase. Lipase has a wide range of actions and participates in multiple reactions, and they can catalyze the hydrolysis of triacylglycerol into its component free fatty acids and glycerol backbone. Lipase exists widely in nature, most prominently in plants, animals and microorganisms, among which microorganisms are the most important source of lipase. Microbial lipases have been adapted for numerous industrial applications due to their substrate specificity, heterogeneous patterns of expression and versatility (i.e., capacity to catalyze reactions at the extremes of pH and temperature as well as in the presence of metal ions and organic solvents). Now they have been introduced into applications involving the production and processing of food, pharmaceutics, paper making, detergents, biodiesel fuels, and so on. In this mini-review, we will focus on the most up-to-date research on microbial lipases and their commercial and industrial applications. We will also discuss and predict future applications of these important technologies.

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“…Combining the benefits of directed evolution and rational design, semi‐rational design approaches create “smart” libraries on the basis of prior structural or functional data that are more likely to yield positive results (Cheng et al, 2015; Chica et al, 2005). Several successful examples of semi‐rational approaches to improve enzymatic properties in OSs have been reported in the literature, for example, for lipase (Tian et al, 2017; Ulrich et al, 2017) and laccase (Rasekh et al, 2014). Interestingly, the OS resistance of enzymes can be substantially increased by introducing only one to five substitutions (Bornscheuer et al, 2018; Chen & Arnold, 1991; Stepankova et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Enabling biocatalysis in high‐concentration organic cosolvent by enzyme gate engineering

Cheng

Wei

et al. 2021

Biotech & Bioengineering

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Biocatalysis in high-concentration organic solvents (OSs) offers many advantages, but realizing this process remains a huge challenge. An R-selective ω-amine transaminase variant (AcATA M2 ) exhibited high activity toward 50 g/L pro-sitagliptin ketone 1-[1-piperidinyl]-4-[2,4,5-trifluorophenyl]-1,3-butanedione (PTfpB). However, AcATA M2 displayed unsatisfactory organic-cosolvent resistance against highconcentration dimethyl sulfoxide (DMSO), which is required to enhance the solubility of the hydrophobic substrate PTfpB. Located in the substrate-binding tunnel, enzyme gates are structural elements that undergo reversible conformational transitions, thus affecting the accessibility of the binding pocket to solvent molecules.Depending on the conformation of the enzyme gates, one can define an open or closed conformation on which the enzyme activity in OSs may depend. To enhance the DMSO resistance of AcATA M2 , we identified the beneficial residues at the "enzyme gate" region via computational analysis, alanine scanning, and sitesaturation mutagenesis. Two beneficial variants, namely, AcATA M2 F56D and AcA-TA M2 F56V , not only displayed improved enzyme activity but also exhibited enhanced DMSO resistance (the half-life value increased from 25.71 to 42.49 h under 60% DMSO). Molecular dynamic simulations revealed that the increase in DMSO resistance was mainly caused by the decrease in the number of DMSO molecules in the substrate-binding pocket. Moreover, in the kilogram-scale experiment, the conversion of 80 g/L substrate was increased from 50% (AcATA M2 ) to 85% (M2 F56D in 40% DMSO) with a high e.e. of >99% within 24 h.

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Directed evolution of Thermomyces lanuginosus lipase to enhance methanol tolerance for efficient production of biodiesel from waste grease

Cited by 69 publications

References 44 publications

Biodiesel and green diesel generation: an overview

Biodiesel and green diesel generation: an overview

A Valuable Product of Microbial Cell Factories: Microbial Lipase

Enabling biocatalysis in high‐concentration organic cosolvent by enzyme gate engineering

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