Directed evolution of artificial repeat proteins as habit modifiers for the morphosynthesis of (111)-terminated gold nanocrystals

Prasad, Janak; Viollet, Sébastien; Gurunatha, Kargal L.; Urvoas, Agathe; Fournier, Agathe C.; Valerio-Lepiniec, Marie; Marcelot, Cécile; Baris, Bulent; Minard, Philippe; Dujardin, Erik

doi:10.1039/c9nr04497c

Cited by 5 publications

(4 citation statements)

References 71 publications

(67 reference statements)

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“…Amino acids containing aromatic or sp 2 hybridized atoms, such as arginine, were identified to have a higher preference for binding to (111) vs (100) facets in MD simulations . Similarly, proteins selected on the basis of preferential binding to gold (111) facets using directed evolution displayed a high frequency of lysine and arginine . While methionine is also known to have a strong binding energy to gold surfaces, we have not found literature supporting the selectivity of methionine for (111) surfaces.…”

Section: Resultsmentioning

confidence: 63%

“…43 Similarly, proteins selected on the basis of preferential binding to gold (111) facets using directed evolution displayed a high frequency of lysine and arginine. 44 While methionine is also known to have a strong binding energy to gold surfaces, we have not found literature supporting the selectivity of methionine for (111) surfaces. Therefore, we hypothesized that in the absence of methionine, the remaining arginine and lysine residues in Z2M246I result in a higher preference for binding to the (111) facet of gold nanomaterials during synthesis.…”

Section: ■ Experimental Materials and Methodsmentioning

confidence: 59%

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Anisotropic Gold Nanomaterial Synthesis Using Peptide Facet Specificity and Timed Intervention

Lachowski,

Chiang,

Torkelson

et al. 2023

Langmuir

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Thin metal particles with two-dimensional (2D) symmetry are attractive for multiple applications but are difficult to synthesize in a reproducible manner. Although molecules that selectively adsorb to facets have been used to control nanoparticle shape, there is still limited research into the temporal control of growth processes to control these structural outcomes. Moreover, much of the current research into the growth of thin 2D particles lacks mechanistic details. In this work, we study why the substitution of isoleucine for methionine in a gold-binding peptide (Z2, RMRMKMK) results in an increase in gold nanoparticle anisotropy. Nanoplatelet growth in the presence of Z2M246I (RIRIKIK) is characterized using in situ small-angle X-ray scattering (SAXS) and UV−vis spectroscopy. Fitting time-resolved SAXS profiles reveal that 10 nm-thick particles with 2D symmetry are formed within the first few minutes of the reaction. Next, through a combination of electron diffraction and molecular dynamics simulations, we show that substitution of methionine for isoleucine increases the (111) facet selectivity in Z2M246I, and we conclude that this is key to the growth of nanoplatelets. However, the potential application of nanoplatelets formed using Z2M246I is limited due to their uncontrolled lateral growth, aggregation, and rapid sedimentation. Therefore, we use a liquid-handling robot to perform temporally controlled synthesis and dynamic intervention through the addition of Z2 to nanoplatelets grown in the presence of Z2M246I at different times. UV−vis spectroscopy, dynamic light scattering, and electron microscopy show that dynamic intervention results in control over the mean size and stability of plate-like particles. Finally, we use in situ UV−vis spectroscopy to study plate-like particle growth at different times of intervention. Our results demonstrate that both the selectivity and magnitude of binding free energy toward lattices are important for controlling nanoparticle growth pathways.

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Section: Resultsmentioning

confidence: 63%

Section: ■ Experimental Materials and Methodsmentioning

confidence: 59%

Anisotropic Gold Nanomaterial Synthesis Using Peptide Facet Specificity and Timed Intervention

Lachowski,

Chiang,

Torkelson

et al. 2023

Langmuir

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“…There is actually a continuum of NP-protein complex residence times encompassing a biologically relevant range that extends from seconds to hours, or even days. Previous studies have conducted quantitative assessments of the binding and unbinding kinetics between individual proteins and 'conventional' NPs [6,54,61,[77][78][79][80][81][82][83][84][85][86][87][88][89]. Figure 4A presents a compilation of the apparent k on and k off rate constants obtained from these investigations (for individual values, refer to Supplementary Table S1).…”

Section: Dissociation Kinetics and Residence Timesmentioning

confidence: 99%

“…binding and unbinding kinetics between individual proteins and 'conventional' NPs [6,54,61,[77][78][79][80][81][82][83][84][85][86][87][88][89]. Figure 4A presents a compilation of the apparent kon and koff rate constants obtained from these investigations (for individual values, refer to Supplementary Table S1).…”

Section: Dissociation Kinetics and Residence Timesmentioning

confidence: 99%

Kinetics and Timescales in Bio–Nano Interactions

Lima,

Sousa

2023

Physchem

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Engineered nanoparticles (NPs) have the potential to revolutionize disease diagnostics and treatment. However, NP interactions with proteins in biological fluids complicate their in vivo control. These interactions often lead to the formation of protein coronas around the NP surface, shaping NP fate and behavior within biological systems. To harness the full potential of NPs in biomedical applications, it is therefore essential to gain a comprehensive understanding of their interactions with proteins. Within this context, it must be recognized that traditional equilibrium-based descriptions of NP–protein interactions, which encompass parameters like equilibrium binding affinity and corona composition, do not provide sufficient detail to predict NP behavior in vivo. This limitation arises because the open in vivo system is a nonequilibrium state characterized by constantly changing concentrations and dynamic regulation of biological processes. In light of these considerations, this review explores the kinetics and timescales of NP–protein interactions, discussing their relevance, fundamental concepts, measurement techniques, typical ranges of association and dissociation rate constants, and dynamics of protein corona formation and dissociation. The review concludes by outlining potential areas for further research and development in this field.

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Phage Display Methodologies

Urvoas

Minard

Soumillion

2020

Springer Protocols Handbooks

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Directed evolution of artificial repeat proteins as habit modifiers for the morphosynthesis of (111)-terminated gold nanocrystals

Abstract: Artificial repeat proteins are selected by directed evolution for their high chemical affinity for gold and structural selectivity for (111) facets. The proteins chaperone the growth of (111)-terminated nanocrystals and form a functional shell.

Cited by 5 publications

References 71 publications

Anisotropic Gold Nanomaterial Synthesis Using Peptide Facet Specificity and Timed Intervention

Anisotropic Gold Nanomaterial Synthesis Using Peptide Facet Specificity and Timed Intervention

Kinetics and Timescales in Bio–Nano Interactions

Phage Display Methodologies

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