2015
DOI: 10.7243/2054-717x-2-1
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Directed differentiation protocols for successful human intestinal organoids derived from multiple induced pluripotent stem cell lines

Abstract: Background: Relatively little has been reported comparing the ability of different induced pluripotent stem cells (iPSCs) and protocols to derive human intestinal organoids (HIO), although there is potential to supply HIO for translational research and regenerative medicine. In view of the time and effort required to differentiate HIO, protocols for differentiation were compared and five iPSC lines, produced by retroviral or non-viral reprogramming methods, were concurrently differentiated to HIO to evaluate t… Show more

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Cited by 10 publications
(7 citation statements)
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References 34 publications
(59 reference statements)
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“…Recently, several researchers have reported that intestinal organoids can be differentiated from human pluripotent stem cells in vitro (Forster et al., 2014, Kauffman et al., 2015, Spence et al., 2011, Tamminen et al., 2015). Human intestinal organoids consist of all small intestinal cell types (paneth cells, goblet cells, enterocytes, and enteroendocrine cells).…”
Section: Introductionmentioning
confidence: 99%
“…Recently, several researchers have reported that intestinal organoids can be differentiated from human pluripotent stem cells in vitro (Forster et al., 2014, Kauffman et al., 2015, Spence et al., 2011, Tamminen et al., 2015). Human intestinal organoids consist of all small intestinal cell types (paneth cells, goblet cells, enterocytes, and enteroendocrine cells).…”
Section: Introductionmentioning
confidence: 99%
“…Recently, 3D organoids and tissue models have been generated from iPSCs to resemble organ structure and function. Examples of complex tissues and organs modelled in 3D include Engineered Heart Tissues (EHTs) [ 5 ], Lung [ 6 ], and intestinal organoids [ 7 ], as well as so-called “mini-brains” [ 8 ]. A 3D model of the vascular system was only recently published [ 9 ].…”
Section: Development and Progress Of Human Ipsc Technology Over The Lmentioning
confidence: 99%
“…Furthermore, since SARS-CoV-2 infections also cause dysfunction of disparate human organs including the lungs, heart, brain, kidney, intestine, etc., disease modeling by employing the human induced pluripotent stem cells (hiPSC) derived organoidmodel systems [30][31][32][33][34][35][36][37][38][39][40], lead the path towards the mechanisms underlying organ failures due to SARS-CoV-2 infections. Such experimental investigations using hiPSC derived organoid models could well generate molecular drug therapies to treat preemptive tissue damage by SARS-CoV-2 infections.…”
Section: Hypothesesmentioning
confidence: 99%