Direct Writing of Tunable Living Inks for Bioprocess Intensification

Qian, Fang; Zhu, Cheng; Knipe, Jennifer M.; Ruelas, Samantha; Stolaroff, Joshuah K.; DeOtte, Joshua R.; Duoss, Eric B.; Spadaccini, Christopher M.; Henard, Calvin A.; Guarnieri, Michael T.; Baker, Sarah E.

doi:10.1021/acs.nanolett.9b00066

Cited by 80 publications

(98 citation statements)

References 26 publications

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“…3D‐printing technologies have been extensively used in different research fields such as energy storage devices, catalysis, electronics, microfluidics, and biotechnology . These printing technologies have enabled the creation of unique material and device structures that cannot be achieved by conventional methods .…”

Section: Methodsmentioning

confidence: 99%

3D‐Printed Structure Boosts the Kinetics and Intrinsic Capacitance of Pseudocapacitive Graphene Aerogels

et al. 2020

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The performance of pseudocapacitive electrodes at fast charging rates are typically limited by the slow kinetics of Faradaic reactions and sluggish ion diffusion in the bulk structure. This is particularly problematic for thick electrodes and electrodes highly loaded with active materials. Here, a surface‐functionalized 3D‐printed graphene aerogel (SF‐3D GA) is presented that achieves not only a benchmark areal capacitance of 2195 mF cm−2 at a high current density of 100 mA cm−2 but also an ultrahigh intrinsic capacitance of 309.1 µF cm−2 even at a high mass loading of 12.8 mg cm−2. Importantly, the kinetic analysis reveals that the capacitance of SF‐3D GA electrode is primarily (93.3%) contributed from fast kinetic processes. This is because the 3D‐printed electrode has an open structure that ensures excellent coverage of functional groups on carbon surface and facilitates the ion accessibility of these surface functional groups even at high current densities and large mass loading/electrode thickness. An asymmetric device assembled with SF‐3D GA as anode and 3D‐printed GA decorated with MnO2 as cathode achieves a remarkable energy density of 0.65 mWh cm−2 at an ultrahigh power density of 164.5 mW cm−2, outperforming carbon‐based supercapacitors operated at the same power density.

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Section: Methodsmentioning

confidence: 99%

3D‐Printed Structure Boosts the Kinetics and Intrinsic Capacitance of Pseudocapacitive Graphene Aerogels

et al. 2020

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“…Calcium alginate and other polysaccharides are the most common matrices used for immobilizing cells, despite the sensitivity of the ionic crosslinks to the presence of charge-bearing molecules and the pH of the medium 18 . Various other hydrogel materials, comprised of synthetic or modified naturally occurring polymers, have been explored but fail to produce a material that is simultaneously readily processable, mechanically robust, and inert to the chemicals and biochemicals present in the media [19][20][21][22][23][24][25][26][27][28][29] .…”

mentioning

confidence: 99%

Compartmentalized microbes and co-cultures in hydrogels for on-demand bioproduction and preservation

et al. 2020

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Most mono-and co-culture bioprocess applications rely on large-scale suspension fermentation technologies that are not easily portable, reusable, or suitable for on-demand production. Here, we describe a hydrogel system for harnessing the bioactivity of embedded microbes for on-demand small molecule and peptide production in microbial mono-culture and consortia. This platform bypasses the challenges of engineering a multi-organism consortia by utilizing a temperature-responsive, shear-thinning hydrogel to compartmentalize organisms into polymeric hydrogels that control the final consortium composition and dynamics without the need for synthetic control of mutualism. We demonstrate that these hydrogels provide protection from preservation techniques (including lyophilization) and can sustain metabolic function for over 1 year of repeated use. This approach was utilized for the production of four chemical compounds, a peptide antibiotic, and carbohydrate catabolism by using either mono-cultures or co-cultures. The printed microbe-laden hydrogel constructs' efficiency in repeated production phases, both pre-and post-preservation, outperforms liquid culture.

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“…These are made from biocompatible materials such as hydrogels, gelatin or alginate and are designed to cross-link immediately after or during bioprinting (Gungor-Ozkerim et al, 2018). They are seeded with living cells which can be printed directly into the desired 3D conformation (Connell et al, 2013;Schaffner et al, 2017;Huang et al, 2018;Qian et al, 2019). Structures can be designed to increase mass transfer, leading to improvements in product yield (Qian et al, 2019) and distinct populations can be layered on top of one another (Lehner et al, 2017;Schmieden et al, 2018).…”

Section: Liquid and Solid State Environmentsmentioning

confidence: 99%

From Microbial Communities to Distributed Computing Systems

Karkaria¹,

Treloar²,

Barnes³

et al. 2020

Front. Bioeng. Biotechnol.

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A distributed biological system can be defined as a system whose components are located in different subpopulations, which communicate and coordinate their actions through interpopulation messages and interactions. We see that distributed systems are pervasive in nature, performing computation across all scales, from microbial communities to a flock of birds. We often observe that information processing within communities exhibits a complexity far greater than any single organism. Synthetic biology is an area of research which aims to design and build synthetic biological machines from biological parts to perform a defined function, in a manner similar to the engineering disciplines. However, the field has reached a bottleneck in the complexity of the genetic networks that we can implement using monocultures, facing constraints from metabolic burden and genetic interference. This makes building distributed biological systems an attractive prospect for synthetic biology that would alleviate these constraints and allow us to expand the applications of our systems into areas including complex biosensing and diagnostic tools, bioprocess control and the monitoring of industrial processes. In this review we will discuss the fundamental limitations we face when engineering functionality with a monoculture, and the key areas where distributed systems can provide an advantage. We cite evidence from natural systems that support arguments in favor of distributed systems to overcome the limitations of monocultures. Following this we conduct a comprehensive overview of the synthetic communities that have been built to date, and the components that have been used. The potential computational capabilities of communities are discussed, along with some of the applications that these will be useful for. We discuss some of the challenges with building co-cultures, including the problem of competitive exclusion and maintenance of desired community composition. Finally, we assess computational frameworks currently available to aide in the design of microbial communities and identify areas where we lack the necessary tools.

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Direct Writing of Tunable Living Inks for Bioprocess Intensification

Cited by 80 publications

References 26 publications

3D‐Printed Structure Boosts the Kinetics and Intrinsic Capacitance of Pseudocapacitive Graphene Aerogels

3D‐Printed Structure Boosts the Kinetics and Intrinsic Capacitance of Pseudocapacitive Graphene Aerogels

Compartmentalized microbes and co-cultures in hydrogels for on-demand bioproduction and preservation

From Microbial Communities to Distributed Computing Systems

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