Direct-write laser micromachining and universal surface modification of PMMA for device development

Cheng, Ji-Yen; Wei, C. M.; Hsu, Kai-Hsiung; Young, Tai‐Horng

doi:10.1016/j.snb.2003.10.022

Cited by 232 publications

(157 citation statements)

References 22 publications

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“…The detail of the PMMA laser machining technique has been reported in our previous works. 32,40,41 The microfluidic network shown in Fig. 1(a) was patterned by CO 2 laser ablation on a piece of 70 lm-thick polyester double-sided tape (PET 8018, 3 M, USA) adhered on a Teflon film (ASF-110, Chukoh Ltd., Japan).…”

Section: Fabrication Of Mfuf Chipmentioning

confidence: 99%

Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field

Tsai

Peng

et al. 2012

Biomicrofluidics

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We report a new design of microfluidic chip (Multiple electric Field with Uniform Flow chip, MFUF chip) to create multiple electric field strengths (EFSs) while providing a uniform flow field simultaneously. MFUF chip was fabricated from poly-methyl methacrylates (PMMA) substrates by using CO2 laser micromachining. A microfluidic network with interconnecting segments was utilized to de-couple the flow field and the electric field (EF). Using our special design, different EFSs were obtained in channel segments that had an identical cross-section and therefore a uniform flow field. Four electric fields with EFS ratio of 7.9:2.8:1:0 were obtained with flow velocity variation of only 7.8% CV (coefficient of variation). Possible biological effect of shear force can therefore be avoided. Cell behavior under three EFSs and the control condition, where there is no EF, was observed in a single experiment. We validated MFUF chip performance using lung adenocarcinoma cell lines and then used the chip to study the electrotaxis of HSC-3, an oral squamous cell carcinoma cell line. The MFUF chip has high throughput capability for studying the EF-induced cell behavior under various EFSs, including the control condition (EFS = 0).

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Section: Fabrication Of Mfuf Chipmentioning

confidence: 99%

Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field

Tsai

Peng

et al. 2012

Biomicrofluidics

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“…The PMMA layer was fabricated by CO 2 laser direct writing. 27 A transparent indium tin oxide ͑ITO͒ heating layer is stacked beneath the PMMA layer. The ITO heater was patterned by laser-induced backside wet etching.…”

Section: A Chip Design and Fabricationmentioning

confidence: 99%

“…In our laboratory, we have developed several rapid-prototyping methods for fabricating microfluidic chips 27,28 and integrated transparent heaters. 29,30 In this work we report a simple design that provides a variably controllable chemical gradient for cell culturing.…”

Section: Introductionmentioning

confidence: 99%

A transparent cell-culture microchamber with a variably controlled concentration gradient generator and flow field rectifier

et al. 2008

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Real-time observation of cell growth provides essential information for studies such as cell migration and chemotaxis. A conventional cell incubation device is usually too clumsy for these applications. Here we report a transparent microfluidic device that has an integrated heater and a concentration gradient generator. A piece of indium tin oxide ͑ITO͒ coated glass was ablated by our newly developed visible laser-induced backside wet etching ͑LIBWE͒ so that transparent heater strips were prepared on the glass substrate. A polymethylmethacrylate ͑PMMA͒ microfluidic chamber with flow field rectifiers and a reagent effusion hole was fabricated by a CO 2 laser and then assembled with the ITO heater so that the chamber temperature can be controlled for cell culturing. A variable chemical gradient was generated inside the chamber by combining the lateral medium flow and the flow from the effusion hole. Successful culturing was performed inside the device. Continuous long-term ͑Ͼ10 days͒ observation on cell growth was achieved. In this work the flow field, medium replacement, and chemical gradient in the microchamber are elaborated.

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“…The pattern was drawn in AutoCAD (Autodesk) and then loaded into a CO 2 laser scriber (M-300, Universal Laser Systems or ILS2, Laser Tools & Technics Corp.) to ablate desired patterns on PMMA substrates. [53][54][55] Three layers ( Fig. 1(a), thickness ¼ 1 mm) were bound together via double-sided tapes (8018, 3M) to form the integrated chip ( Fig.…”

Section: A Chip Design and Fabricationmentioning

confidence: 99%

Effects of shear stresses and antioxidant concentrations on the production of reactive oxygen species in lung cancer cells

Zhu

Tsai

et al. 2013

Biomicrofluidics

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Reactive oxygen species (ROS) are known to be a key factor in the development of cancer, and many exogenous sources are supposed to be related to the formation of ROS. In this paper, a microfluidic chip was developed for studying the production of ROS in lung cancer cells under different chemical and physical stimuli. This chip has two unique features: (1) five relative concentrations of 0, 1/8, 1/2, 7/8, and 1 are achieved in the culture regions; (2) a shear stress gradient is produced inside each of the five culture areas. Lung cancer cells were seeded inside this biocompatible chip for investigating their response to different concentrations of H 2 O 2 , a chemical stimulus known to increase the production of ROS. Then the effect of shear stress, a physical stimulus, on lung cancer cells was examined, showing that the production of ROS was increased in response to a larger shear stress. Finally, two antioxidants, a-tocopherol and ferulic acid, were used to study their effects on reducing ROS. It was found that high-dose a-tocopherol was not able to effectively eliminate the ROS produced inside cells. This counter effect was not observed in cells cultured in a traditional chamber slide, where no shear stress was present. This result suggests that the current microfluidic chip provides an in vitro platform best mimicking the physiological condition where cells are under circulating conditions. V C 2013 AIP Publishing LLC. [http://dx

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Direct-write laser micromachining and universal surface modification of PMMA for device development

Cited by 232 publications

References 22 publications

Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field

Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field

A transparent cell-culture microchamber with a variably controlled concentration gradient generator and flow field rectifier

Effects of shear stresses and antioxidant concentrations on the production of reactive oxygen species in lung cancer cells

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