Direct transfer of vitrified rabbit embryos

Vicente, J.S.; García-Ximénez, F.

doi:10.1016/0093-691x(96)00010-6

Cited by 20 publications

(7 citation statements)

References 15 publications

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“…To produce a control population, embryos from the base generation were vitrified and stored in liquid N 2 until transfer. Details of the technique are presented by Vicente and García-Ximénez (1996). After thawing and transferring the embryos, a total of 26 does of the control group was obtained, and these control does were contemporary to the does from the high and the low lines of Generation 11 ( Table 1).…”

Section: Animalsmentioning

confidence: 99%

Divergent selection for uterine capacity in rabbits. II. Correlated response in litter size and its components estimated with a cryopreserved control population1

Santacreu

Mocé

Climent

et al. 2005

Journal of Animal Science

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Our objective was to evaluate the correlated responses to selection for litter size and its components after 10 generations of divergent selection for uterine capacity (UC). A total of 294 intact females from the 11th and 12th generations of divergent selection for high and low UC and from a cryopreserved control population was used (139, 112, and 43 females, respectively). Uterine capacity was assessed as litter size in unilaterally ovariectomized females. Traits recorded on females for up to five parities were litter size (LS) and number born alive (NBA). Laparoscopy was performed in all females at d 12 of their second parity, and the ovulation rate (OR) and number of implanted embryos (IE) were recorded in these females. Embryo survival (ES = IE/OR), fetal survival (FS = LS/IE), and prenatal survival (PS = LS/OR) were computed. Correlated responses in LS and in its components were inferred using Bayesian methods. Correlated responses in LS were asymmetric. The divergence between high and low lines was 2.35 kits, mainly because of a higher correlated response in the low line (1.88 kits). The lower LS in the low line was associated with a lower PS (control - low = 0.14), because of decreases in ES and FS.

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Section: Animalsmentioning

confidence: 99%

Divergent selection for uterine capacity in rabbits. II. Correlated response in litter size and its components estimated with a cryopreserved control population1

Santacreu

Mocé

Climent

et al. 2005

Journal of Animal Science

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“…After one generation without selection, to avoid the environmental maternal effect, 72 adult V16 female rabbits were obtained to be simultaneously compared with 79 female rabbits of generation 36. Detailed information concerning the cryopreservation and embryo transfer techniques used in this study are available in García-Ximénez (1993 and1996).…”

Section: Animalsmentioning

confidence: 99%

Different resource allocation strategies result from selection for litter size at weaning in rabbit does

Savietto

Cervera

Ródenas

et al. 2014

Animal

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This study examined the effect of long-term selection of a maternal rabbit line, solely for a reproductive criterion, on the ability of female rabbits to deal with constrained environmental conditions. Female rabbits from generations 16 and 36 (n = 72 and 79, respectively) of a line founded and selected to increase litter size at weaning were compared simultaneously. Female rabbits were subjected to normal (NC), nutritional (NF) or heat (HC) challenging conditions from 1st to 3rd parturition. Animals in NC and NF were housed at normal room temperatures (18°C to 25°C) and respectively fed with control (11.6 MJ digestible energy (DE)/kg dry matter (DM), 126 g digestible protein (DP)/kg DM, and 168 g of ADF/kg DM) or low-energy fibrous diets (9.1 MJ DE/kg DM, 104 g DP/kg DM and 266 g ADF/kg DM), whereas those housed in HC were subjected to high room temperatures (25°C to 35°C) and the control diet. The litter size was lower for female rabbits housed in both NF and HC environments, but the extent and timing where this reduction took place differed between generations. In challenging conditions (NF and HC), the average reduction in the reproductive performance of female rabbits from generation 16, compared with NC, was −2.26 (P < 0.05) and −0.51 kits born alive at 2nd and 3rd parturition, respectively. However, under these challenging conditions, the reproductive performance of female rabbits from generation 36 was less affected at 2nd parturition (−1.25 kits born alive), but showed a greater reduction at the 3rd parturition (−3.53 kits born alive; P < 0.05) compared with NC. The results also showed differences between generations in digestible energy intake, milk yield and accretion, and use of body reserves throughout lactation in NC, HC and NF, which together indicate that there were different resource allocation strategies in the animals from the different generations. Selection to increase litter size at weaning led to increased reproductive robustness at the onset of an environmental constraint, but failure to sustain the reproductive liability when the challenge was maintained in the long term. This response could be directly related to the shortterm environmental fluctuations (less severe) that frequently occur in the environment where this line has been selected.

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“…Among the factors responsible for this situation a visible tendency of less frequent development of in vitro cultured, traditionally vitrified embryos to more advanced blastocyst stages (83.1% vs. 90.6% in control, Table 2) may be indicated. Nevertheless, the ratio of pups born after vitrification shown here (36.8%) remains within the range of data reported recently for rabbit cryopreserved embryo transfer (Vicente and Garcia Ximenez, 1996;Kaufmann et al, 1998;Lopez-Bejar and Lopez-Gatius, 2002;Mehaisen et al, 2006). Further experiments will be needed to compare the transfer efficiency of embryos vitrified using methods presented in this paper (especially those more promising, employing the VitMaster system).…”

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confidence: 57%

Comparison of traditional and modified (VitMaster) methods of rabbit embryo vitrification

Papis

Korwin-Kossakowski

Wenta-Muchalska

2009

Acta Veterinaria Hungarica

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In spite of their cryobiological efficacy, minimum-volume vitrification methods suffer from the risk of microbiological contamination and are technically and/or manually demanding. In this study, the effects of a traditional, slightly modified vitrification method and vitrification using supercooled liquid nitrogen (VitMaster) applied for rabbit morula-stage embryos were compared. Embryos were equilibrated in a solution containing 1,2-propanediol (2.72 M) and glycerol (1.36 M) for 7 min and vitrified in 0.25-ml insemination straws after 1-min exposure to a vitrification solution containing additionally 1.0 M sucrose. Cooling was performed in 'normal' or supercooled liquid nitrogen. Regardless of the cooling method applied, high in vitro survival and development rates of vitrified embryos were obtained. All embryos were intact after warming, and 61 out of 65 (93.8%) and 23 out of 24 (95.8%) embryos developed to the blastocyst stage after 48-h in vitro culture of embryos vitrified in 'normal' or supercooled liquid nitrogen, respectively. The results suggest higher developmental ability of embryos vitrified in supercooled liquid nitrogen (91.7% vs. 83.1% of embryos vitrified traditionally developed to more advanced, expanding and/or hatching blastocyst stages). In vivo survival rate, tested for the traditional vitrification system only, revealed that 36.8% of embryos developed to term. The results show promise for establishing a fully successful method for rabbit embryo vitrification.

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Direct transfer of vitrified rabbit embryos

Cited by 20 publications

References 15 publications

Divergent selection for uterine capacity in rabbits. II. Correlated response in litter size and its components estimated with a cryopreserved control population1

Divergent selection for uterine capacity in rabbits. II. Correlated response in litter size and its components estimated with a cryopreserved control population1

Different resource allocation strategies result from selection for litter size at weaning in rabbit does

Comparison of traditional and modified (VitMaster) methods of rabbit embryo vitrification

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