Direct transfer of frozen-thawed bovine embryos and its application in cattle reproduction management

Dochi, O.

doi:10.1262/jrd.2019-025

Cited by 27 publications

(28 citation statements)

References 48 publications

(76 reference statements)

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“…As described previously ( 40 ), blastocysts imaged by OCT were transferred to a cryoprotective solution [1.8 M of ethylene glycol and 0.1 M of sucrose in Dulbecco's PBS (D-PBS)], which was then placed in a 0.25-mL straw (IMV Technologies, L'Aigle, France) at room temperature. After the blastocysts were equilibrated at room temperature for 15 min, the straws were directly set in a programmable freezer (ET-1N; FUJIHIRA INDUSTRY CO., LTD, Tokyo, Japan) at −7°C where seeding was manually performed.…”

Section: Methodsmentioning

confidence: 99%

Three-Dimensional Live Imaging of Bovine Preimplantation Embryos: A New Method for IVF Embryo Evaluation

Masuda¹,

Hasebe

Kuromi

et al. 2021

Front. Vet. Sci.

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Conception rates for transferred bovine embryos are lower than those for artificial insemination. Embryo transfer (ET) is widely used in cattle but many of the transferred embryos fail to develop, thus, a more effective method for selecting bovine embryos suitable for ET is required. To evaluate the developmental potential of bovine preimplantation embryos (2-cell stage embryos and blastocysts), we have used the non-invasive method of optical coherence tomography (OCT) to obtain live images. The images were used to evaluate 22 parameters of blastocysts, such as the volume of the inner cell mass and the thicknesses of the trophectoderm (TE). Bovine embryos were obtained by in vitro fertilization (IVF) of the cumulus-oocyte complexes aspirated by ovum pick-up from Japanese Black cattle. The quality of the blastocysts was examined under an inverted microscope and all were confirmed to be Code1 according to the International Embryo Transfer Society standards for embryo evaluation. The OCT images of embryos were taken at the 2-cell and blastocyst stages prior to the transfer. In OCT, the embryos were irradiated with near-infrared light for a few minutes to capture three-dimensional images. Nuclei of the 2-cell stage embryos were clearly observed by OCT, and polynuclear cells at the 2-cell stage were also clearly found. With OCT, we were able to observe embryos at the blastocyst stage and evaluate their parameters. The conception rate following OCT (15/30; 50%) is typical for ETs and no newborn calves showed neonatal overgrowth or died, indicating that the OCT did not adversely affect the ET. A principal components analysis was unable to identify the parameters associated with successful pregnancy, while by using hierarchical clustering analysis, TE volume has been suggested to be one of the parameters for the evaluation of bovine embryo. The present results show that OCT imaging can be used to investigate time-dependent changes of IVF embryos. With further improvements, it should be useful for selecting high-quality embryos for transfer.

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Section: Methodsmentioning

confidence: 99%

Three-Dimensional Live Imaging of Bovine Preimplantation Embryos: A New Method for IVF Embryo Evaluation

Masuda¹,

Hasebe

Kuromi

et al. 2021

Front. Vet. Sci.

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“…As described previously [28], blastocysts imaged by OCT were transferred to a cryoprotective solution (1.8 M ethylene glycol and 0.1 M sucrose in Dulbecco's phosphate buffered saline [D-PBS]), which was then placed in a 0.25 ml straw (IMV Technologies, L'Aigle, France) at room temperature (25°C). After the blastocysts were equilibrated at room temperature for 15 min, the straws were directly set in a programmable freezer (ET-1N;…”

Section: Cryopreservationmentioning

confidence: 99%

Three-dimensional live imaging of bovine embryos by optical coherence tomography

Masuda¹,

Hasebe

Kuromi

et al. 2021

J. Reprod. Dev.

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While embryo transfer (ET) is widely practiced, many of the transferred embryos fail to develop in cattle. To establish a more effective method for selecting bovine embryos for ET, here we quantified morphological parameters of living embryos using three-dimensional (3D) images non-invasively captured by optical coherence tomography (OCT). Seven Japanese Black embryos produced by in vitro fertilization that had reached the expanded blastocyst stage after 7 days of culture were transferred after imaged by OCT. Twenty-two parameters, including thickness and volumes of the inner cell mass, trophectoderm, and zona pellucida, and volumes

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“…In some instances, recipients are available for a limited time (i.e. milking dairy cows) and because DT is a quicker process, more transfers can be performed without interfering with a commercial daily operation (Dochi, 2019; Voelkel & Hu, 1992).…”

Section: Ivp Embryo Cryotolerancementioning

confidence: 99%

Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

Ferré

Kjelland

Taiyeb³

et al. 2020

Reprod Domestic Animals

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Contents Cryopreservation of in vitro‐derived bovine embryos is a crucial step for the widespread reproduction and conservation of valuable high‐merit animals. Given the current popularity of bovine in vitro embryo production (IVP), there is a demand for a highly efficient ultra‐low temperature storage method in order to maximize donor ovum pickup (OPU) turn‐over, recipient availability/utilization and domestic/overseas commercial trading opportunities. However, IVP bovine embryos are still very sensitive to chilling and cryopreservation, and despite recent progress, a convenient (simple and robust) protocol has not yet been developed. At the moment, there are two methods for bovine IVP embryo cryopreservation: slow programmable freezing and vitrification. Both of the aforementioned techniques have pros and cons. While controlled‐rate slow cooling can easily be adapted for direct transfer (DT), ice crystal formation remains an issue. On the other hand, vitrification solved this problem but the possibility of successful DT commercial incorporation remains to be determined. Moreover, simplification of the vitrification protocol (including warming) through the use of an in‐straw dilution without the use of a microscope is a prerequisite for its use under farm conditions. This review summarizes the bovine IVP embryo cryopreservation achievements, strengths and limitations of both freezing systems and prospective improvements to enhance cryosurvival, as well as perspectives on future directions of this assisted reproductive technology.

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Direct transfer of frozen-thawed bovine embryos and its application in cattle reproduction management

Cited by 27 publications

References 48 publications

Three-Dimensional Live Imaging of Bovine Preimplantation Embryos: A New Method for IVF Embryo Evaluation

Three-Dimensional Live Imaging of Bovine Preimplantation Embryos: A New Method for IVF Embryo Evaluation

Three-dimensional live imaging of bovine embryos by optical coherence tomography

Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

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