Direct Transcriptional Control of a p38 MAPK Pathway by the Circadian Clock in Neurospora crassa

Lamb, Teresa M.; Goldsmith, Charles S.; Bennett, Lindsay; Finch, Katelyn E.; Bell-Pedersen, Deborah

doi:10.1371/journal.pone.0027149

Cited by 36 publications

(50 citation statements)

References 72 publications

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“…In studies to identify output pathways from the clock, we discovered that the WCC rhythmically binds to the promoter of os-4, encoding the OS-4 MAPKKK, and drives rhythms in the accumulation of os-4 mRNA and OS-4 protein. Rhythmic accumulation of OS-4 protein is necessary for rhythmic phosphorylation and activation of the terminal MAPK, OS-2, and downstream ccgs (20,21). Taken together, these data demonstrate that clock control of the OS pathway provides a mechanism for Neurospora to prepare for predictable daily hyperosmotic stresses.…”

mentioning

confidence: 68%

“…Integration at the mak-1 locus was verified by PCR (data not shown). Circadian time course experiments were performed in liquid culture as previously described (21), with the following modifications. Mycelial mats from the ⌬mak-1 strain required 4 additional days of growth in constant light (LL) compared to wild-type strains, because of poor growth.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, clock regulation of major signaling pathways, such as mitogen-activated protein kinase (MAPK) pathways, provides mechanisms for circadian regulation of large sets of functionally related downstream target genes of the pathway. For example, we previously found that the Neurospora FWO controls rhythmic activity of the osmolarity-sensitive (OS) MAPK pathway and downstream ccg targets of the pathway (20)(21)(22).…”

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confidence: 99%

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Circadian Activation of the Mitogen-Activated Protein Kinase MAK-1 Facilitates Rhythms in Clock-Controlled Genes in Neurospora crassa

et al. 2013

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The circadian clock regulates the expression of many genes involved in a wide range of biological functions through output pathways such as mitogen-activated protein kinase (MAPK) pathways. We demonstrate here that the clock regulates the phosphorylation, and thus activation, of the MAPKs MAK-1 and MAK-2 in the filamentous fungus Neurospora crassa. In this study, we identified genetic targets of the MAK-1 pathway, which is homologous to the cell wall integrity pathway in Saccharomyces cerevisiae and the extracellular signal-regulated kinase 1/2 (ERK1/2) pathway in mammals. When MAK-1 was deleted from Neurospora cells, vegetative growth was reduced and the transcript levels for over 500 genes were affected, with significant enrichment for genes involved in protein synthesis, biogenesis of cellular components, metabolism, energy production, and transcription. Additionally, of the ϳ500 genes affected by the disruption of MAK-1, more than 25% were previously identified as putative clock-controlled genes. We show that MAK-1 is necessary for robust rhythms of two morning-specific genes, i.e., ccg-1 and the mitochondrial phosphate carrier protein gene NCU07465. Additionally, we show clock regulation of a predicted chitin synthase gene, NCU04352, whose rhythmic accumulation is also dependent upon MAK-1. Together, these data establish a role for the MAK-1 pathway as an output pathway of the circadian clock and suggest a link between rhythmic MAK-1 activity and circadian control of cellular growth.

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confidence: 68%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Circadian Activation of the Mitogen-Activated Protein Kinase MAK-1 Facilitates Rhythms in Clock-Controlled Genes in Neurospora crassa

et al. 2013

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“…Interestingly, the N. crassa HPt homolog is indirectly regulated by the circadian clock (734). T. reesei, T. atroviride, and T. virens also have three response regulator (RR) proteins related to SSK1, SKN7, and RIM15 or RRG-1, RRG-2, and STK-12 in N. crassa (see Table S1 in the supplemental material).…”

Section: Protein Kinasesmentioning

confidence: 99%

“…Intriguingly, in N. crassa, the photoreceptor complex WCC binds to the os-4 promoter in response to light and influences OS-2 phosphorylation rhythms (734). Hence, an influence of light and the circadian clock on the Trichoderma TMK3/HOG1 (TR_45018, TA_301235, and TV_83666) pathway would not be without precedent.…”

Section: Protein Kinasesmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

159

195

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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Circadian Rhythms in Liver Physiology and Liver Diseases

Tong

Yin

2013

Comprehensive Physiology

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In mammals, circadian rhythms function to coordinate a diverse panel of physiological processes with environmental conditions such as food and light. As the driving force for circadian rhythmicity, the molecular clock is a self-sustained transcription-translational feedback loop system consisting of transcription factors, epigenetic modulators, kinases/phosphatases, and ubiquitin E3 ligases. The molecular clock exists not only in the suprachiasmatic nuclei of the hypothalamus but also in the peripheral tissues to regulate cellular and physiological function in a tissue-specific manner. The circadian clock system in the liver plays important roles in regulating metabolism and energy homeostasis. Clock gene mutant animals display impaired glucose and lipid metabolism and are susceptible to diet-induced obesity and metabolic dysfunction, providing strong evidence for the connection between the circadian clock and metabolic homeostasis. Circadian-controlled hepatic metabolism is partially achieved by controlling the expression and/or activity of key metabolic enzymes, transcription factors, signaling molecules, and transporters. Reciprocally, intracellular metabolites modulate the molecular clock activity in response to the energy status. Although still at the early stage, circadian clock dysfunction has been implicated in common chronic liver diseases. Circadian dysregulation of lipid metabolism, detoxification, reactive oxygen species (ROS) production, and cell-cycle control might contribute to the onset and progression of liver steatosis, fibrosis, and even carcinogenesis. In summary, these findings call for a comprehensive study of the function and mechanisms of hepatic circadian clock to gain better understanding of liver physiology and diseases.

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Direct Transcriptional Control of a p38 MAPK Pathway by the Circadian Clock in Neurospora crassa

Cited by 36 publications

References 72 publications

Circadian Activation of the Mitogen-Activated Protein Kinase MAK-1 Facilitates Rhythms in Clock-Controlled Genes in Neurospora crassa

Circadian Activation of the Mitogen-Activated Protein Kinase MAK-1 Facilitates Rhythms in Clock-Controlled Genes in Neurospora crassa

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Circadian Rhythms in Liver Physiology and Liver Diseases

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