Direct TLC-MALDI Coupling Using a Hybrid Plate

Mehl, John T; Hercules, David M.

doi:10.1021/ac990003y

Cited by 58 publications

(45 citation statements)

References 24 publications

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“…In another study, GSLs developed on a TLC plate were transferred to a PVDF membrane then coupled to MALDI MS; this was called the TLC-Blot-MALDI MS method [35][36][37]. There have been only a few recent reports on TLC-Blot-MALDI MS, because the additional work, i.e., the transfer from TLC plates to PVDF membranes, requires, and the orthogonal configuration of the instrument allows for direct desorption of samples from the irregular surface of the TLC plate without impairment of either mass accuracy or resolution [38].…”

Section: Introductionmentioning

confidence: 99%

High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes

Goto‐Inoue

Hayasaka

Sugiura

et al. 2008

Journal of Chromatography B

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Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. Here we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e. both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports. (10 pmol)

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Section: Introductionmentioning

confidence: 99%

High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes

Goto‐Inoue

Hayasaka

Sugiura

et al. 2008

Journal of Chromatography B

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“…The direct coupling described previously has been implemented on axial MALDI-TOF mass spectrometers, [30][31][32][33], showing the feasibility of this method. However, axial MALDI-TOF instruments require a flat surface for sample desorption to achieve optimum resolving power and mass accuracy results.…”

mentioning

confidence: 98%

Ganglioside analysis by thin-layer chromatography matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry

Ivleva

Sapp

O’Connor

et al. 2005

J. Am. Soc. Mass Spectrom.

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Metastable decomposition of ions generated in matrix-assisted laser desorption/ionization (MALDI) mass spectrometers complicates analysis of biological samples that have labile bonds. Recently, several academic laboratories and manufacturers of commercial instruments have designed instruments that introduce a cooling gas into the ion source during the MALDI event and have shown that the resulting vibrational cooling stabilizes these labile bonds. In this study, we compared stabilization and detection of desorbed gangliosides on a commercial orthogonal time-of-flight (oTOF) instrument with results we reported previously that had been obtained on a home-built Fourier transform mass spectrometer. Decoupling of the desorption/ ionization from the detection steps resulted in an opportunity for desorbing thin-layer chromatography (TLC)-separated gangliosides directly from a TLC plate without compromising mass spectral accuracy and resolution of the ganglioside analysis, thus coupling TLC and oTOF mass spectrometry. The application of a declustering potential allowed control of the matrix cluster and matrix adduct formation, and, thus, enhanced the detection of the gangliosides. during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analyses of molecules having labile bonds. Although this phenomenon can be used for obtaining analyte structural information for small peptides, oligosaccharides, and some glycolipids [1-5] for larger or more fragile molecules, it can limit the mass accuracy and resolution and complicate interpretation of the spectra of heterogeneous samples. Thus, it is important to develop methods that allow control of the extent of metastable decay. In addition to the pressure of the background gas and extraction field strength in the MALDI technique, the major factors affecting metastable fragmentation are laser wavelength/pulse width and the choice of the MALDI matrix [6,7]. In addition, the presence of electrons may play a role in analyte fragmentation and may cause suppression of multiply charged ions in a spectrum [8,9].Control of the metastable fragmentation in MALDI by increasing the pressure in the ionization source and reduction of the ions internal energy was first introduced by Loboda et al. on orthogonal time-of-flight (oTOF) instruments [10] and later extended to Fourier transform mass spectrometry (FTMS) [11] and to the operation at atmospheric pressure on various types of instruments [12]. These methods have since been used for stabilization of glycolipids [13,14] and peptides and proteins [11,15]. The term vibrational cooling (VC) MALDI recently has been introduced to describe desorption in the pressure range where cooling of the excess bond energy is achieved [12]. Stabilization of the labile bonds under these conditions allows for control of the extent of fragmentation, which is particularly

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“…The challenge of the TLC-MS coupling, which is different from the other chromatography-MS approaches, arises between the extraction from the plate and introduction into the mass spectrometer, because the sample is embedded within the stationary phase. Therefore, most common methods developed for direct sampling TLC-MS are today based on surface or laser desorption/ionization techniques ( 37,38 ). However, because of the high risk for elimination of sialic acid ( 39 ), unless special devices are employed, such as the vibrationally cooled MALDI-FTMS system mentioned above ( 40 ) or a MALDI-Q-o-TOF MS that can be operated at relatively high ion source pressure ( 29 ), gangliosides cannot be individually analyzed.…”

mentioning

confidence: 99%

Direct analysis of sialylated or sulfated glycosphingolipids and other polar and neutral lipids using TLC-MS interfaces

Park¹,

Zhou²,

Costello

2014

Journal of Lipid Research

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Direct TLC-MALDI Coupling Using a Hybrid Plate

Cited by 58 publications

References 24 publications

High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes

High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes

Ganglioside analysis by thin-layer chromatography matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry

Direct analysis of sialylated or sulfated glycosphingolipids and other polar and neutral lipids using TLC-MS interfaces

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