Direct technetium-99m labeling of anti-hepatoma monoclonal antibody fragment: a radioimmunoconjugate for hepatocellular carcinoma imaging

Function of HAb18G/CD147 in Invasion of Host Cells by Severe Acute Respiratory Syndrome Coronavirus

“…To analyze the interaction between HAb18G/CD147 and CyPA, 100 mg of HAb18G/CD147 (a recombinant extracellular fragment of HAb18G/CD147, prepared in our lab) was incubated with an equivalent amount of CyPA (Alexis Biochemical) overnight at room temperature (RT). The mixture was then added to the gel, which was coupled with 200 mg of mouse anti-human HAb18G/CD147 monoclonal antibody (MAb) HAb18 (prepared in our lab) [25][26][27][28][29][30][31][32][33], for incubation with gentle end-over-end mixing for 4 h. After the bound proteins were eluted, aliquots of the eluent were analyzed by a Western blot developed with rabbit anti-human CyPA antibody (diluted 1:2000; Calbiochem-Novabiochem) and horseradish peroxidase (HRP)conjugated goat anti-rabbit IgG (diluted 1:5000; Amersham Pharmacia). Purchased CyPA (2.5 mg) was used as the positive control.…”

Radioimmunoscintigraphy of pancreatic cancer in tumor-bearing athymic nude mice using 99mtechnetium-labeled anti-KL-6/MUC1 antibody

“…[8][9][10]13,14 In the future, we need to generate F(ab)2 or scFv fragments of the monoclonal antibody to avoid or reduce its antigenicity for use in human subjects, which has already been carried out for monoclonal anti-carcinoembryonic antigen antibody. 32…”

“…Labeling of monoclonal antibodies with 99m Tc has been found to be effective for radioimmunoscintigraphy. [8][9][10][11]14 The antibody was labeled with 99m Tc as described by Karube et al 8 with minor modifi cations. Anti-KL-6/ MUC1 antibody (2.5 mg) was reduced by reaction with 2-mercaptoethanol at room temperature for 60 min.…”

“…[3][4][5] Specifi c monoclonal antibodies are extremely powerful tools for the diagnosis or treatment of some diseases; for example, daclizumab (a monoclonal antibody against the α chain of the interleukin 2 receptor) has been used to prevent acute rejection after renal transplantation 6 and rituximab (a monoclonal antibody against CD20) has been used to treat lymphomas. 7 Radiolabeling of tumorspecifi c antibodies for use as in vivo tracers has been attempted to detect the distribution of neoplasms by scintigraphy, [8][9][10][11] and one of these approaches is now in clinical use. 12 Mucins comprise a group of high-molecular-weight glycoproteins.…”