Direct stability-indicating method development and validation for analysis of etidronate disodium using a mixed-mode column and charged aerosol detector

Simultaneous determination of positive and negative pharmaceutical counterions using mixed-mode chromatography coupled with charged aerosol detector

“…The columns that exhibit both reversed-phase and ion-exchange (anionic, cationic or zwitterionic) behavior are most common type of mixed-mode columns [14][15][16][17][18][19]. Recently, mixed-mode columns with HILIC (hydrophilic interaction liquid chromatography) behavior have become increasingly popular in the separation of highly polar molecules including ions being weakly or not retained by conventional reversed-phase LC systems [20][21][22][23][24].…”

Development of a nucleotide sugar purification method using a mixed mode column & mass spectrometry detection

“…Results demonstrated that pH affected retention time. The Primesep SB column is always positively charged throughout the pH range of the mobile phases explored in this study [21]. While the buffer range for ammonium formate is 2.8-4.8, the pH values tested in this study were in the range of 2.0-5.0.…”

“…Among the several Primesep columns available, the Primesep SB column contains strong basic ion-pairing groups, improves retention of acidic compounds by anion-exchange mechanism, separates bases by ion-exclusion mechanism together with hydrophobicity and retains neutral compounds via a reversed-phase mechanism [20]. It has been shown to resolve analytes that are very polar and have highly ionic characteristics, such as etidronate disodium [21]. The column was used for stability-indicating method development and validation for analysis of etidronate disodium [21].…”

“…It has been shown to resolve analytes that are very polar and have highly ionic characteristics, such as etidronate disodium [21]. The column was used for stability-indicating method development and validation for analysis of etidronate disodium [21]. Another study showed successful separation of nucleotides and sugar phosphates [10].…”

Impurity profiling of ibandronate sodium by HPLC–CAD

“…Instead, the non-volatile impurities 3 and 4, the related substances 7, 9 and 11 as well as the amino acid precursor 1 of ibandronate (Fig. 2) can be subjected to particle based detectors like ELSD, CNLSD or CAD [21][22][23][24][25][26][27]. Due to the known disadvantages of the ELSD when it comes to impurity profiling, like non reproducible spike peaks and poor sensitivity [28][29][30], the CAD was chosen as detector because it does not show artefacts and provides better sensitivity.…”