Direct Somatic Embryogenesis in &lt;I&gt;Coffea canephora&lt;/I&gt;

Quiroz-Figueroa, Francisco Roberto; Monforte‐González, Miriam; Galáz-Ávalos, Rosa M.; Loyola‐Vargas, Víctor M.

doi:10.1385/1-59259-959-1:111

Cited by 30 publications

(31 citation statements)

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“…After a 14‐day pretreatment, four fully developed leaves were excised from each plantlet, and five 0.8 cm diameter explants were cut from each leaf. Disc explants were then incubated in Yasuda liquid media, as modified by Quiroz‐Figueroa et al (), in the presence of 5 μM benzyladenine (BA, Sigma‐Aldrich) and then cultivated at 25 ± 2°C in the dark in an orbital shaker at 55 rpm. Five discs explants were cultivated in the absence of BA as negative controls.…”

Section: Methodsmentioning

confidence: 99%

Ectopic expression of the Coffea canephora SERK1 homolog‐induced differential transcription of genes involved in auxin metabolism and in the developmental control of embryogenesis

Pérez-Pascual

Jiménez-Guillen

Villanueva-Alonzo

et al. 2018

Physiologia Plantarum

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Somatic embryogenesis receptor-like kinase 1 (SERK1) is a membrane receptor that might serve as common co-regulator of plant cell differentiation processes by forming heterodimers with specific receptor-like kinases. The Coffea canephora SERK1 homolog (CcSERK1) was cloned in this work, and its early function in the transcription of embryogenesis master genes and of genes encoding proteins involved in auxin metabolism was investigated by externally manipulating its expression in embryogenic leaf explants, before the appearance of embryogenic structures. Overexpression of CcSERK1 early during embryogenesis caused an increase in the number of somatic embryos when the 55-day process was completed. Suppression of CcSERK1 expression by RNA interference almost abolished somatic embryogenesis. Real time-PCR experiments revealed that the transcription of the CcAGL15, CcWUS, CcBBM, CcPKL, CcYUC1, CcPIN1 and CcPIN4 homologs was modified in direct proportion to the expression of CcSERK1 and that only CcLEC1 was inversely affected by the expression levels of CcSERK1. The expression of the CcYUC4 homolog was induced to more than 80-fold under CcSERK1 overexpression conditions, but it was also induced when CcSERK1 expression was silenced. The level of CcTIR1 was not affected by CcSERK1 overexpression but was almost abolished during CcSERK1 silencing. These results suggest that CcSERK1 co-regulates the induction of somatic embryogenesis in Coffea canephora by early activation of YUC-dependent auxin biosynthesis, auxin transport mediated by PIN1 and PIN4, and probably auxin perception by the TIR1 receptor, leading to the induction of early-stage homeotic genes (CcAGL15, CcWUS, CcPKL and CcBBM) and repression of late-stage homeotic genes (CcLec1).

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Section: Methodsmentioning

confidence: 99%

Ectopic expression of the Coffea canephora SERK1 homolog‐induced differential transcription of genes involved in auxin metabolism and in the developmental control of embryogenesis

Pérez-Pascual

Jiménez-Guillen

Villanueva-Alonzo

et al. 2018

Physiologia Plantarum

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“…of Abies alba (European silver fir). 13 Other plants, such as Coffea canephora, 14 Medicago spp., 15,16 and Manihot esculenta Crantz 17 also require the addition of an auxin to induce SE. The increase in IAA conjugates is related to the presence of enzymes with acyl-amidosynthetase activity; they are codified by genes belonging to the GRETCHEN HAGEN 3 (GH3) family.…”

Section: Dynamics Of the Concentration Of Iaa And Some Of Its Conjugamentioning

confidence: 99%

“…We have shortened the embryogenic response time in SE of C. canephora to 5 wk. 14 Since an exogenous auxin is not added during the induction of SE, this system is excellent to study the internal auxins during the induction of SE in C. canephora.…”

Section: Dynamics Of the Concentration Of Iaa And Some Of Its Conjugamentioning

confidence: 99%

“…Plantlets of C. canephora were obtained from somatic embryos as previously reported 14 and cultured in Murashige and Skoog 39 (MS; PhytoTechnology Laboratories, M524) medium, supplemented with 29.6 μM thiamine-HCl (Sigma, T3902-25G), 550 μM myo-inositol (Sigma, I5125-500G), 0.15 μM cysteine (Sigma, C-8277), 87.64 mM sucrose (Sigma, S5391) and 0.25% (w/v) gelrite (Sigma, G1910), pH 5.8 and cultured at 25 ± 2 °C under a 16/8h (light/darkness) photoperiod (150 μmol m -2 s -1 ) ( Fig. S1).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis inCoffea canephora

Ayil-Gutiérrez

Galáz-Ávalos

Peña‐Cabrera

et al. 2013

Plant Signaling & Behavior

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Most of the somatic embryogenesis (SE) process requires the presence, either before or during the embryogenic process, of at least one exogenous auxin. This exogenous auxin induces the presence of endogenous auxins, which appears to be essential for SE induction. We found that during the preincubation period of SE in Coffea canephora, there is an important increase in both free and conjugated indole-3-acetic acid (IAA), as well as indole-3-butyric acid. This increase is accompanied by an increase in the expression of YUCCA (CcYUC), TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (CcTAA1), and GRETCHEN HAGEN 3 (GH3) genes. On the other hand, most of the IAA compounds decreased during the induction of SE. The results presented in this research suggest that a balance between free IAA and its amide conjugates is necessary to allow the expression of SE-related genes.

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“…Owing to the karyotype variation and crop relevance of the HT accessions, in vitro tissue culture techniques such as micro-cutting (Bertrand-Desbrunais et al 1991, Etienne andBertrand 2001) and somatic embryogenesis (SE) (Staritsky 1970, Quiroz-Figueroa et al 2006, Rezende et al 2008 can be used to achieve clonal and mass propagation.…”

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confidence: 99%

Regeneration of Allotriploid <i>Coffea</i> Plants from Tissue Culture: Resolving the Propagation Problems Promoted by Irregular Meiosis

2016

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Summary Híbrido de Timor (HT) is a natural interspecific hybrid of Coffea arabica L. with Coffea canephoraPierre ex Froehner that has played a substantial role in breeding programs as source of resistance genes. The original HT plant is represented by the anorthoploid accession CIFC 4106 , an allotriploid plant with 3x=33 chromosomes and 2C=2.10 pg. From this HT plant, other accessions have been obtained and used in crossings with C. arabica to provide resistant plants to the main pathogens. However, each HT accession and their derivates show a particular karyotype due to irregular anaphasic disjunction, which results in a few reproductive cells with an unbalanced number of chromosomes. Regarding this fact and the crop relevance of HT, this study aimed to develop a direct somatic embryogenesis system for the clonal propagation of this germplasm, using the first HT plant as explant donor. To accomplish this purpose, disinfested leaf explants of greenhouse-cultivated HT CIFC 4106 plants were inoculated in medium supplied with 0.001 g L 1 6-benzylaminopurine. Somatic embryogenesis process was asynchronous, with distinct developmental stages occurring simultaneously. Besides, emergence of secondary embryos from primary ones was observed. Mature embryos were germinated, and well-rooted embryos were selected for plantlet regeneration. The nuclear DNA content and the karyotype showed that allotriploidy was conserved in all regenerated plantlets. Considering these results, the direct somatic embryogenesis protocol adopted in the present work was imperative for the accurate clonal propagation of HT, being relevant for multiplication and conservation of elite accessions that show an irregular meiosis.

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Direct Somatic Embryogenesis in <I>Coffea canephora</I>

Cited by 30 publications

References 5 publications

Ectopic expression of the Coffea canephora SERK1 homolog‐induced differential transcription of genes involved in auxin metabolism and in the developmental control of embryogenesis

Ectopic expression of the Coffea canephora SERK1 homolog‐induced differential transcription of genes involved in auxin metabolism and in the developmental control of embryogenesis

Dynamics of the concentration of IAA and some of its conjugates during the induction of somatic embryogenesis inCoffea canephora

Regeneration of Allotriploid <i>Coffea</i> Plants from Tissue Culture: Resolving the Propagation Problems Promoted by Irregular Meiosis

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