Direct single cell determination of nitric oxide synthase related metabolites in identified nitrergic neurons

Moroz, Leonid L.; Dahlgren, Robin L.; Boudko, Dmitry; Sweedler, Jonathan V.; Lovell, Peter V.

doi:10.1016/j.jinorgbio.2005.01.013

Cited by 50 publications

(79 citation statements)

References 76 publications

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“…This antisense region enables the transcript of the pseudogene to form a heteroduplex with the NOS mRNA, which suppresses NOS mRNA translation. The suggestion that CGCs are unable to produce NO is also supported by a recent study, which showed a low concentration of NOS-related metabolites in the CGC (Moroz et al 2005). However, it should be noted that the level of NOS and pseudo-NOS mRNA expression is dynamically regulated in the CGCs as reported in a recent publication showing that appetitive conditioning causes a transient upregulation in NOS expression and downregulation in pseudo-NOS expression in the CGCs (Korneev et al 2005).…”

Section: Discussionmentioning

confidence: 62%

Modulation of Serotonergic Neurotransmission by Nitric Oxide

Straub

Grant²,

O’Shea³

et al. 2007

Journal of Neurophysiology

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tric oxide (NO) and serotonin (5-HT) are two neurotransmitters with important roles in neuromodulation and synaptic plasticity. There is substantial evidence for a morphological and functional overlap between these two neurotransmitter systems, in particular the modulation of 5-HT function by NO. Here we demonstrate for the first time the modulation of an identified serotonergic synapse by NO using the synapse between the cerebral giant cell (CGC) and the B4 neuron within the feeding network of the pond snail Lymnaea stagnalis as a model system. Simultaneous electrophysiological recordings from the pre-and postsynaptic neurons show that blocking endogenous NO production in the intact nervous system significantly reduces the B4 response to CGC activity. The blocking effect is frequency dependent and is strongest at low CGC frequencies. Conversely, bath application of the NO donor DEA/NONOate significantly enhances the CGC-B4 synapse. The modulation of the CGC-B4 synapse is mediated by the soluble guanylate cyclase (sGC)/cGMP pathway as demonstrated by the effects of the sGC antagonist 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). NO modulation of the CGC-B4 synapse can be mimicked in cell culture, where application of 5-HT puffs to isolated B4 neurons simulates synaptic 5-HT release. Bath application of diethylamine NONOate (DEA/NONOate) enhances the 5-HT induced response in the isolated B4 neuron. However, the cell culture experiment provided no evidence for endogenous NO production in either the CGC or B4 neuron suggesting that NO is produced by an alternative source. Thus we conclude that NO modulates the serotonergic CGC-B4 synapse by enhancing the postsynaptic 5-HT response.

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Section: Discussionmentioning

confidence: 62%

Modulation of Serotonergic Neurotransmission by Nitric Oxide

Straub

Grant²,

O’Shea³

et al. 2007

Journal of Neurophysiology

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“…It is present in endothelial and neuronal cells at relatively steady state high concentrations of up to 1-2 mM [100,101], which implies existence of an active and regulated transport system. The intracellular L-arginine pool is generated by mean activity of protein digestion/ synthesis pathways, amino acid metabolism [102], and secondary plasma membrane transporters.…”

Section: Nos Substrate L-arginine-l-mentioning

confidence: 99%

“…Conductivity-based assays were also optimized in a number of practical works relevant to the analysis of the L-arginine/NO pathway [101,179,[247][248][249][313][314][315][316][317]. The major challenge of CD/CCD in the determination of nitrite and nitrate in biological samples is the high concentration of Cl − .…”

Section: Contact and Contactless Conductivity Detection (Cd Ccd C 4mentioning

confidence: 99%

“…The type of combination of CZE and ITP sample treatment can be especially useful for planar chip CE technologies such as the one introduced by Kaniansky and colleagues [318]. Our group has optimized a CZE/CD technique to measure nitrite and nitrate concentrations in hemolymph, tissues, and individual neurons samples from model mollusks [101,315], subepithelial and epithelial tissue microsamples from the midgut of mosquito larvae [249], and submicroliter tissue samples from mammalian neuronal tissues [247]. To reduce chloride interference and improve NO 2 − detection, we use sample cleanup with a home-made solid-phase microextraction (SPME) cartridge filled with Dionex OnGuard-Ag cationic exchange resin.…”

Section: Contact and Contactless Conductivity Detection (Cd Ccd C 4mentioning

confidence: 99%

“…Recently a more comprehensive evaluation was performed for the simultaneous determination of L-arginine, L-citrulline, NO 2 − and NO 3 − in a selected set of NO-ergic and NO-naïve molluscan neurons [101]. HPCE/LIF of OPF-derivatized samples was used for the amino acid assay, and the CZE-CD technique was used for inorganic anions (Fig.…”

Section: Single-neuron and Neuronal Tissue Hpce Of No Metabolitesmentioning

confidence: 99%

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Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

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This review briefly summarizes recent progress in fundamental understanding and analytical profiling of the L-arginine/nitric oxide (NO) pathway. It focuses on key analytical references of NO actions and on the experimental acquisition of these references in vivo, with capillary electrophoresis (CE) and high-performance capillary electrophoresis (HPCE) comprising one of the most flexible and technologically promising analytical platform for comprehensive high-resolution profiling of NO-related metabolites. Second aim of this review is to express demands and bridge efforts of experimental biologists, medical professionals and chemical analysis-oriented scientists who strive to understand evolution and physiological roles of NO and to develop analytical methods for use in biology and medicine.

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2008

Coupled Bioluminescent Assays

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Direct single cell determination of nitric oxide synthase related metabolites in identified nitrergic neurons

Cited by 50 publications

References 76 publications

Modulation of Serotonergic Neurotransmission by Nitric Oxide

Modulation of Serotonergic Neurotransmission by Nitric Oxide

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

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