Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort.

Martin, K. P.; Joseph, Dominic; Madasser, Joseph; Philip, V. J.

doi:10.1079/ivp2003460

Cited by 59 publications

(45 citation statements)

References 12 publications

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“…This result can be explained with the presence of predetermined cells which first dedifferentiate themselves, subsequently form new meristematic centres, and finally differentiate again producing new organs [34][35][36][37][38][39]. This process, due to cell totipotency, has been observed in several species [30,[40][41][42][43][44][45][46][47] and is very efficient for regeneration. Our results showed that AFLP is a very sensitive and reliable molecular marker technique for revealing specific genomic alterations induced by tissue culture and for identifying slightly different genotypes.…”

Section: Discussionmentioning

confidence: 99%

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Perrini

Alba

Ruta

et al. 2009

Cellular and Molecular Biology Letters

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A protocol for the induction of regeneration from leaves of Helichrysum italicum was established. Calli were found to form on the basal medium only when it was supplemented with thidiazuron (TDZ) alone or in combination with naphthalene acetic acid (NAA), with a percentage ranking of at least 80%. The hormone-free medium showed the highest percentage of shoot regeneration (62%) even though no callus formed. AFLP markers were employed to verify tissue culture-induced variation in the regenerated plantlets obtained by direct shoot regeneration or the indirect shoot regeneration process (callus formation). Seven out of the eleven AFLP primer pairs yielded polymorphic patterns. The average number of fragments per primer pair was 64.1. Singletons were represented by 12 (2.7%) fragments. Student’s T-test was performed both on the average number of shared fragments and on the nucleotide diversity, and no significant statistical difference was observed between the two regeneration treatments.

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Section: Discussionmentioning

confidence: 99%

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Perrini

Alba

Ruta

et al. 2009

Cellular and Molecular Biology Letters

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“…A number of researchers have examined the process involved in the administration of minerals and the results suggest a complex network of interactions between the explant and the culture medium (Ramage and Williams, 2002). In A. andreanum, adventitious shoots of Calypso were obtained in 1/2 MS, a result which is similar to those obtained in Anthurium antioquiense (Murillo-Gómez et al, 2014), A. andreanum cv Tinora Red and Senator (Martin et al, 2003), A. andreanum cv flamingo (Viégas et al, 2007) and A. andreanum (Jahan et al, 2009). There are a number of reports in which the induction of shoots in A. andreanum has been possible using MS at 100% of its ionic strength (Sedaghati et al, 2012); this is possible due to the fact that the morphogenic response varies according to the genotype, which would suggest the need to develop protocols for each variety (Gantait and Mandal, 2010).…”

Section: Morphogenesis and Plant Regenerationmentioning

confidence: 63%

Morphogenesis and plant regeneration from Anthurium andreanum cv Calypso leaf explant

Guadalupe¹,

Mayanin²

2017

Afr. J. Biotechnol.

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Anthuriums are among the most attractive ornamental plants; however, the commercial production of these plants is limited by the slow propagation methods presently in use. This situation can be resolved with the application of in vitro culture techniques which allow massive plant propagation through morphogenic processes. Plant growth regulators (PGR) and the composition of the basal media comprising the culture medium are among the factors influencing the induction of morphogenesis. Optical and electron microscopy analysis suggested that the morphogenic routes induced were organogenesis and somatic embryogenesis. This report presents three protocols of morphogenesis, two for adventitious shoot organogenesis and one via somatic embryogenesis. The treatments which induced adventitious shoot organogenesis were Murashige and Skoog medium at half ionic strength supplemented with 0.2 μM of thidiazuron (TDZ) and 2.2 μM of 6-benzylaminopurine (BAP); 0.2 μM of TDZ and 6.6 μM of zeatin (ZEA); the treatment which induced somatic embryogenesis was 0.4 μM of TDZ and 0.5 μM of 2, 4-dichlorophenoxyacetic acid (2, 4-D).

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“…Desde el establecimiento de las primeras técnicas para la multiplicación, diversos grupos han innovado en el procedimiento (Kunisaki, 1980;Geier, 1986;Teng, 1997;Chen et al, 1997;Joseph et al, 2003;Martin et al, 2003;Vargas et al, 2004: Yixun et al, 2009.…”

Section: Introductionunclassified

Reprogramación celular de embriones de Anthurium andraeanum por fitohormonas para micropropagación masiva

Mireles-Ordaz

Arellano-Perusquia

Espinal-Centeno

et al. 2015

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El género Anthurium incluye alrededor de 800 especies, las cuales son originarias de diversos países tropicales y subtropicales de América. Numerosos cultivos de estas especies de Anthurium son crecidos y comercializados debido a su gran popularidad como plantas ornamentales alrededor de todo el mundo, de las cuales la más popular es Anthurium andreanum. La reproducción sexual de estas plantas en invernaderos es difícil y toma mucho tiempo, lo cual representa una desventaja para su producción masiva y comercialización. La micropropagación in vitro ha emergido como una opción para sobrellevar dicha desventaja, hasta la fecha se han logrado avances parcialmente exitosos en la micropropagación en especies de Anthurium usando varios tejidos como explantes iniciales, incluyendo hojas, pecíolo, espádice, espata, brote lateral, meristemo apical y embriones somáticos, estos últimos previamente inducidos a partir de tejido diferenciado. Sin embargo, hasta ahora no hay reportes del uso de embriones cigóticos como tejido madre para la propagación masiva de estas plantas. En el presente estudio reportamos la formación de callos organogénicos a partir de embriones cigóticos inmaduros crecidos en medio MS suplementado con la combinación de 2 mg/l de ácido 2,4-diclorofenoxiacético (2,4-D) y 0.5 mg/l de 6-benzilamino-purina (BAP). Los resultados obtenidos demuestran que los embriones en proceso de desarrollo son altamente eficientes como explantes de origen para inducir la formación de tejido calloso de una manera rápida y fácil, dando en promedio 9 brotes por callo y logrando una taza de sobrevivencia de las plantas del 90% en la fase de aclimatación.

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Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort.

Cited by 59 publications

References 12 publications

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Morphogenesis and plant regeneration from Anthurium andreanum cv Calypso leaf explant

Reprogramación celular de embriones de Anthurium andraeanum por fitohormonas para micropropagación masiva

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