Direct Serum Total Iron-binding Capacity Assay Suitable for Automated Analyzers

Siek, Gordon; Lawlor, Joseph; Pelczar, Donna; Sane, Meena; Musto, Joseph D.

doi:10.1093/clinchem/48.1.161

Cited by 25 publications

(7 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The negative proportional bias was ~8%, meaning that results gained by the Olympus »calculation method« were 91.9% of the results obtained by the reference method. These findings are similar with those reported in other studies (9,(12)(13)(14).…”

Section: Discussionsupporting

confidence: 93%

Reference interval for calculated total iron-binding capacity using Olympus AU2700 analyzer

Jovicic

Ignjatović

Dajak

et al. 2005

Jugoslav Med Biohem

View full text Add to dashboard Cite

Total iron-binding capacity (TIBC) values are determined on Olympus AU2700 automated chemistry analyzer as the sum of serum iron and unsaturated iron-binding capacity (UIBC)-(calculated TIBC, TIBC cal). Considering that Olympus AU2700 automated analyzer was recently brought in function and TIBC values calculated from serum iron and UIBC values were significantly lower than those obtained by a direct and fully automated TIBC assay, it was necessary to determine the reference interval for TIBC, according to the recommendation that every laboratory should have its own reference ranges. The »calculation method« of TIBC determination showed satisfactory accuracy (p > 0.001) and precision, with CV values ranging from 0.91% to 1.63% within-run and from 2.30% to 2.80% day-today. The correlation between the TIBC values obtained with the »calculation method« using Olympus AU2700 analyzer (y) and those obtained with the direct method (x) was: y = 0.919x + 2.319 mmol/L (r = 0.980; Sy,x = 1.814; p < 0.001; N = 85). The reference interval for TIBC was determined using sera collected from 125 healthy individuals of both sexes, 15 to 80 years old. Since the values did not depend on sex, the reference interval calculated for the whole studied population ranged between 42.0 mmol/L and 64.3 mmol/L.

show abstract

Section: Discussionsupporting

confidence: 93%

Reference interval for calculated total iron-binding capacity using Olympus AU2700 analyzer

Jovicic

Ignjatović

Dajak

et al. 2005

Jugoslav Med Biohem

View full text Add to dashboard Cite

show abstract

“…A second reagent is then added, making the pH rise, resulting in a large increased affinity of transferrin for iron. The observed decrease in absorbance of the coloured dye-iron complex is directly proportional to the TIBC of the serum [42]. We hypothesize that transferrin from rhinoceroses is not as pH-sensitive as human transferrin, and that pH variations were not adequate to accurately measure TIBC in this species, leading to underestimated values.…”

Section: Plos Onementioning

confidence: 88%

Inflammatory and oxidative status in European captive black rhinoceroses: A link with Iron Overload Disorder?

et al. 2020

View full text Add to dashboard Cite

Iron Overload Disorder (IOD) is a syndrome developed by captive browsing rhinoceroses like black rhinoceroses (Diceros bicornis), in which hemosiderosis develops in vital organs while free iron accumulates in the body, potentially predisposing to various secondary diseases. Captive grazing species like white rhinoceroses (Ceratotherium simum) do not seem to be affected. The authors hypothesized that inflammation and oxidative stress may be implicated in the pathogenesis of IOD in captive black rhinoceroses, making this syndrome a potential common denominator to various diseases described in captivity in this species. In this prospective study, 15 black (BR) and 29 white rhinoceroses (WR) originating from 22 European zoos were blood-sampled and compared for their iron status (serum iron), liver/ muscle biochemical parameters (AST, GGT, cholesterol), inflammatory status (total proteins, protein electrophoresis) and oxidative stress markers (SOD, GPX, dROMs). Results showed higher serum iron and liver enzyme levels in black rhinoceroses (P < 0.01), as well as higher dROMs (P < 0.01) and a trend for higher GPX (P = 0.06) levels. The albumin/globulin ratio was lower in black rhinoceroses (P < 0.05) due to higher α 2-globulin levels (P < 0.001). The present study suggests a higher inflammatory and oxidative profile in captive BR than in WR, possibly in relation to iron status. This could be either a consequence or a cause of iron accumulation. Further investigations are needed to assess the prognostic value of the inflammatory and oxidative markers in captive black rhinoceroses, particularly for evaluating the impact of reduced-iron and antioxidant-supplemented diets.

show abstract

“…A second reagent is then added, making the pH rise, resulting in a large increased affinity of transferrin for iron. The observed decrease in absorbance of the coloured dye-iron complex is directly proportional to the TIBC of the serum [40]. We hypothesized that transferrin from rhinoceroses was not as pH-sensitive as human transferrin, and that pH variations were not adequate to accurately measure TIBC in this species, leading to underestimated values.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory and oxidative status in European captive black rhinoceroses: a link with Iron Overload Disorder?

Pouillevet

Soetart

Boucher

et al. 2020

Preprint

View full text Add to dashboard Cite

17Iron Overload Disorder (IOD) is a syndrome developed by captive browsing 18 rhinoceroses like black rhinoceroses (Diceros bicornis) in which hemosiderosis settles in vital 19 organs while free iron accumulates in the body, potentially predisposing to various secondary 20 diseases. Captive grazing species like white rhinoceroses (Ceratotherium simum) do not seem 21 to be affected. The pro-oxidant and pro-inflammatory properties of iron, associated with the 22 poor antioxidant capacities of black rhinoceroses, could enhance high levels of inflammation 23 and oxidative stress leading to rapid ageing and promoting diseases. In this prospective study, 24 15 black (BR) and 29 white rhinoceroses (WR) originating from 22 European zoos were blood-25 sampled and compared for their iron status (serum iron), liver/muscle biochemical parameters 26 (AST, GGT, cholesterol), inflammatory status (total proteins, protein electrophoresis) and 27 oxidative stress markers (SOD, GPX, dROMs). Results showed higher serum iron and liver 28 enzyme levels in black rhinoceroses (P<0.01), as well as higher GPX (P<0.05) and dROM 29 (P<0.01) levels. The albumin/globulin ratio was lower in black rhinoceroses (P<0.05) due to 30 higher  2 -globulin levels (P<0.001). The present study suggests a higher inflammatory and 31 oxidative profile in captive BR than in WR, possibly in relation to iron status. This could be 32 either a consequence or a cause of iron accumulation, potentially explaining rapid ageing and 33 various diseases. Further investigations are needed to assess the prognostic value of the 34 inflammatory and oxidative markers in captive black rhinoceroses, particularly for evaluating 35 the impact of reduced-iron and antioxidant-supplemented diets. 36

show abstract

Direct Serum Total Iron-binding Capacity Assay Suitable for Automated Analyzers

Cited by 25 publications

References 13 publications

Reference interval for calculated total iron-binding capacity using Olympus AU2700 analyzer

Reference interval for calculated total iron-binding capacity using Olympus AU2700 analyzer

Inflammatory and oxidative status in European captive black rhinoceroses: A link with Iron Overload Disorder?

Inflammatory and oxidative status in European captive black rhinoceroses: a link with Iron Overload Disorder?

Contact Info

Product

Resources

About