Direct reprogramming induces vascular regeneration post muscle ischemic injury

Kaur, Keerat; Hadas, Yoav; Kurian, Achamma; Żak, Magdalena; Yoo, Jimeen; Mahmood, Asharee; Girard, Hanna; Komargodski, Rinat; Io, Toshiro; Santini, Maria Paola; Sultana, Nishat; Sharkar, Mohammad Tofael Kabir; Magadum, Ajit; Fargnoli, Anthony S.; Yoon, Seonghun; Chepurko, Elena; Chepurko, Vadim; Eliyahu, Efrat; Pinto, Dalila; Lebeche, Djamel; Kovacic, Jason C.; Hajjar, Roger J.; Rafii, Shahin; Zangi, Lior

doi:10.1016/j.ymthe.2021.07.014

Cited by 25 publications

(16 citation statements)

References 70 publications

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“…They showed high transgene expression shortly after infection and more efficient generation of beating iCMs when compared to retroviral delivery. As a safe and non-immunogenic gene delivery method, modified mRNA (modRNA) was used to deliver a cocktail including GHMT, dominant-negative (DN)-TGF-β, DN-Wnt8a, and acid ceramidase into mouse fibroblasts ( Kaur et al., 2021 ). Cardiac marker expression showed 57% reprogramming efficiency in vitro after repeated transfection of modRNA mixture during the first two weeks of reprogramming.…”

Section: Methods For Cardiac Differentiationmentioning

confidence: 99%

A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

Lyra-Leite¹,

Gutiérrez-Gutiérrez²,

Wang³

et al. 2022

STAR Protocols

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Section: Methods For Cardiac Differentiationmentioning

confidence: 99%

A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

Lyra-Leite¹,

Gutiérrez-Gutiérrez²,

Wang³

et al. 2022

STAR Protocols

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“…Additionally, CFs can be reprogrammed into CMs and cardiovascular progenitor cells via lentiviral packaging techniques ( Isomi et al, 2021 ) and CRISPR ( Jiang et al, 2022 ), respectively. Moreover, microRNA-delivery platforms efficiently reprogram CFs into induced CMs ( Yang et al, 2021a ) or non-CMs into CMs-like cells ( Kaur et al, 2021 ). Further, photo biomodulation therapy modulates gene transcription and miRNA expression to reverse the profibrotic signaling pathway ( Feliciano et al, 2022 ), and fibroblast cilia regulates cardiac regeneration ( Djenoune et al, 2022 ).…”

Section: Interventions For Cardiac Fibrosismentioning

confidence: 99%

Post-myocardial infarction fibrosis: Pathophysiology, examination, and intervention

Yin

Pan

et al. 2023

Front. Pharmacol.

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Cardiac fibrosis plays an indispensable role in cardiac tissue homeostasis and repair after myocardial infarction (MI). The cardiac fibroblast-to-myofibroblast differentiation and extracellular matrix collagen deposition are the hallmarks of cardiac fibrosis, which are modulated by multiple signaling pathways and various types of cells in time-dependent manners. Our understanding of the development of cardiac fibrosis after MI has evolved in basic and clinical researches, and the regulation of fibrotic remodeling may facilitate novel diagnostic and therapeutic strategies, and finally improve outcomes. Here, we aim to elaborate pathophysiology, examination and intervention of cardiac fibrosis after MI.

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“…This cocktail reprogrammed CM-like cells in the scar area, reduced scar size, promoted capillary density, improved cardiac function, and increased survival after MI. However, it was unable to develop de novo beating CMs either in vitro or in vivo [ 85 ]. This limitation of modRNA might be due to the relatively short transgene expression time that was insufficient to reprogram non-CMs into mature CMs.…”

Section: Cf Reprogramming Into Induced Cmsmentioning

confidence: 99%

Direct Reprogramming of Resident Non-Myocyte Cells and Its Potential for In Vivo Cardiac Regeneration

Perveen

Vanni

Iacono

et al. 2023

Cells

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Cardiac diseases are the foremost cause of morbidity and mortality worldwide. The heart has limited regenerative potential; therefore, lost cardiac tissue cannot be replenished after cardiac injury. Conventional therapies are unable to restore functional cardiac tissue. In recent decades, much attention has been paid to regenerative medicine to overcome this issue. Direct reprogramming is a promising therapeutic approach in regenerative cardiac medicine that has the potential to provide in situ cardiac regeneration. It consists of direct cell fate conversion of one cell type into another, avoiding transition through an intermediary pluripotent state. In injured cardiac tissue, this strategy directs transdifferentiation of resident non-myocyte cells (NMCs) into mature functional cardiac cells that help to restore the native tissue. Over the years, developments in reprogramming methods have suggested that regulation of several intrinsic factors in NMCs can help to achieve in situ direct cardiac reprogramming. Among NMCs, endogenous cardiac fibroblasts have been studied for their potential to be directly reprogrammed into both induced cardiomyocytes and induced cardiac progenitor cells, while pericytes can transdifferentiate towards endothelial cells and smooth muscle cells. This strategy has been indicated to improve heart function and reduce fibrosis after cardiac injury in preclinical models. This review summarizes the recent updates and progress in direct cardiac reprogramming of resident NMCs for in situ cardiac regeneration.

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Direct reprogramming induces vascular regeneration post muscle ischemic injury

Cited by 25 publications

References 70 publications

A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

Post-myocardial infarction fibrosis: Pathophysiology, examination, and intervention

Direct Reprogramming of Resident Non-Myocyte Cells and Its Potential for In Vivo Cardiac Regeneration

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