Direct Regulation of the NADPH Oxidase RBOHD by the PRR-Associated Kinase BIK1 during Plant Immunity

Kadota, Yasuhiro; Sklenář, Jan; Derbyshire, Paul; Stransfeld, Lena; Asai, Shuji; Ntoukakis, Vardis; Jones, Jonathan D. G.; Shirasu, Ken; Menke, Frank L.H.; Jones, Alexandra M. E.; Zipfel, Cyril

doi:10.1016/j.molcel.2014.02.021

Cited by 765 publications

(952 citation statements)

References 68 publications

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“…Bacterial growth assays were performed as described previously (Kadota et al ., 2014), with slight modification. We assessed the significance of CML41 activity in overall plant resistance by first infiltrating leaves of different Arabidopsis lines with the virulent bacterial pathogen P. syringae pv.…”

Section: Methodsmentioning

confidence: 99%

A calmodulin‐like protein regulates plasmodesmal closure during bacterial immune responses

Cheval

Laohavisit

et al. 2017

New Phytologist

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Summary Plants sense microbial signatures via activation of pattern recognition receptors (PPRs), which trigger a range of cellular defences. One response is the closure of plasmodesmata, which reduces symplastic connectivity and the capacity for direct molecular exchange between host cells.Plasmodesmal flux is regulated by a variety of environmental cues but the downstream signalling pathways are poorly defined, especially the way in which calcium regulates plasmodesmal closure.Here, we identify that closure of plasmodesmata in response to bacterial flagellin, but not fungal chitin, is mediated by a plasmodesmal‐localized Ca2+‐binding protein Calmodulin‐like 41 (CML41). CML41 is transcriptionally upregulated by flg22 and facilitates rapid callose deposition at plasmodesmata following flg22 treatment. CML41 acts independently of other defence responses triggered by flg22 perception and reduces bacterial infection.We propose that CML41 enables Ca2+‐signalling specificity during bacterial pathogen attack and is required for a complete defence response against Pseudomonas syringae.

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Section: Methodsmentioning

confidence: 99%

A calmodulin‐like protein regulates plasmodesmal closure during bacterial immune responses

Cheval

Laohavisit

et al. 2017

New Phytologist

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“…BIK1 phosphorylates the NADPH oxidase RbohD to regulate ROS generation (Kadota et al, 2014;. To investigate whether XopR can interfere with the phosphorylation of RbohD by BIK1, we performed an in vitro kinase assay followed by an acrylamide-pendant Phos-tag TM SDS-PAGE analysis.…”

Section: Sds-pagementioning

confidence: 99%

“…BIK1 is involved in PAMP-triggered stomatal closure (Kadota et al, 2014;, and XopR targets BIK1. Therefore, we asked whether XopR could interfere with PAMP-triggered stomatal closure.…”

Section: Xopr Suppresses Pamp-induced Stomatal Closurementioning

confidence: 99%

“…Recently, it has been reported that an RLCK, BIK1, plays a pivotal role in stomatal immunity signaling (Kadota et al, 2014;. BIK1 is a component of the FLS2 immune receptor complex (Lu et al, 2010;.…”

Section: Introductionmentioning

confidence: 99%

“…BIK1 is a component of the FLS2 immune receptor complex (Lu et al, 2010;. It directly phosphorylates the NADPH oxidase RbohD at specific sites, resulting into ROS generation, thus causing the two guard cells of the stomata to actively reduce the opening aperture to prevent the entry of microbes (Kadota et al, 2014;. In addition to FLS2, BAK1 and BIK1, there are other RLKs found to be involved in PTI signaling.…”

Section: Introductionmentioning

confidence: 99%

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A Xanthomonas oryzae pv. oryzae effector, XopR, associates with receptor-like cytoplasmic kinases and suppresses PAMP-triggered stomatal closure

Wang

Sun

Fan

et al. 2016

Sci. China Life Sci.

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Receptor-like kinases (RLKs) play important roles in plant immunity signaling; thus, many are hijacked by pathogen effectors to promote successful pathogenesis. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of rice leaf blight disease. The strain PXO99A has 18 non-TAL (transcription activation-like) effectors; however, their mechanisms of action and host target proteins remain largely unknown. Although the effector XopR from the Xoo strain MAFF311018 was shown to suppress PAMP-triggered immune responses in Arabidopsis, its target has not yet been identified. Here, we show that PXO99A XopR interacts with BIK1 at the plasma membrane. BIK1 is a receptor-like cytoplasmic kinase (RLCK) belonging to the RLK family of proteins and mediates PAMP-triggered stomatal immunity. In turn, BIK1 phosphorylates XopR. Furthermore, XopR suppresses PAMP-triggered stomatal closure in transgenic Arabidopsis expressing XopR. In addition, XopR is able to associate with RLCKs other than BIK1. These results suggest that XopR likely suppresses plant immunity by targeting BIK1 and other RLCKs. Xanthomonas, effector, XopR, BIK1, immunity Citation:Wang, S., Sun, J., Fan, F., Tan, Z., Zou, Y., and Lu, D. (2016). A Xanthomonas oryzae pv. oryzae effector, XopR, associates with receptor-like cytoplasmic kinases and suppresses PAMP-triggered stomatal closure. Sci China Life Sci 59, 897 -905.

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CBL1‐CIPK26‐mediated phosphorylation enhances activity of the NADPH oxidase RBOHC, but is dispensable for root hair growth

et al. 2018

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Root hairs (RH) are tip growing polarized cells aiding the uptake of nutrients and water into plants. RH differentiation involves the interplay of various hormones and second messengers. Tightly regulated production of reactive oxygen species by the NADPH oxidase RBOHC crucially functions in RH differentiation and Ca -dependent phosphorylation has been implemented in these processes. However, the kinases regulating RBOHC remained enigmatic. Here we identify CBL1-CIPK26 Ca sensor-kinase complexes as modulators of RBOHC activity. Combined genetic, cell biological and biochemical analyses reveal synergistic function of CIPK26-mediated phosphorylation and Ca binding for RBOHC activation. Complementation of rbohC mutant RH phenotypes by a S318/322 phosphorylation deficient RBOHC version suggests flexible and alternating phosphorylation patterns as mechanism fine-tuning ROS production in RH development.

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Direct Regulation of the NADPH Oxidase RBOHD by the PRR-Associated Kinase BIK1 during Plant Immunity

Cited by 765 publications

References 68 publications

A calmodulin‐like protein regulates plasmodesmal closure during bacterial immune responses

A calmodulin‐like protein regulates plasmodesmal closure during bacterial immune responses

A Xanthomonas oryzae pv. oryzae effector, XopR, associates with receptor-like cytoplasmic kinases and suppresses PAMP-triggered stomatal closure

CBL1‐CIPK26‐mediated phosphorylation enhances activity of the NADPH oxidase RBOHC, but is dispensable for root hair growth

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