Direct Recording of Trans-Plasma Membrane Electron Currents Mediated by a Member of the Cytochrome <i>b</i>561 Family of Soybean

Picco, Cristiana; Scholz‐Starke, Joachim; Festa, Margherita; Costa, Alex; Sparla, Francesca; Trost, Paolo; Carpaneto, Armando

doi:10.1104/pp.15.00642

Cited by 22 publications

(13 citation statements)

References 38 publications

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“…Combined, these signaling pathways function to control cell expansion via direct modulation of plasma membrane H + ATPase activity and apoplastic acidification (70). Lastly, three auxin responsive cytochrome b561 electron transport proteins were induced in weeping peach shoots, which are thought to stimulate H + ATPase pumps via plasma membrane depolarization (71).…”

Section: Discussionmentioning

confidence: 99%

Loss of a highly conserved sterile alpha motif domain gene ( WEEP ) results in pendulous branch growth in peach trees

Hollender

Pascal

Tabb

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Plant shoots typically grow upward in opposition to the pull of gravity. However, exceptions exist throughout the plant kingdom. Most conspicuous are trees with weeping or pendulous branches. While such trees have long been cultivated and appreciated for their ornamental value, the molecular basis behind the weeping habit is not known. Here, we characterized a weeping tree phenotype in (peach) and identified the underlying genetic mutation using a genomic sequencing approach. Weeping peach tree shoots exhibited a downward elliptical growth pattern and did not exhibit an upward bending in response to 90° reorientation. The causative allele was found to be an uncharacterized gene,, having a 1.8-Kb deletion spanning the 5' end. This gene, dubbed , was predominantly expressed in phloem tissues and encodes a highly conserved 129-amino acid protein containing a sterile alpha motif (SAM) domain. Silencing WEEP in the related tree species (plum) resulted in more outward, downward, and wandering shoot orientations compared to standard trees, supporting a role for WEEP in directing lateral shoot growth in trees. This previously unknown regulator of branch orientation, which may also be a regulator of gravity perception or response, provides insights into our understanding of how tree branches grow in opposition to gravity and could serve as a critical target for manipulating tree architecture for improved tree shape in agricultural and horticulture applications.

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Section: Discussionmentioning

confidence: 99%

Loss of a highly conserved sterile alpha motif domain gene ( WEEP ) results in pendulous branch growth in peach trees

Hollender

Pascal

Tabb

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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“…However, the protein partners responsible for electron transport from cytosolic NADPH to the apoplastic side of the membrane had not been identified previously. Only recently, transplasma membrane electron currents mediated by a soybean cytochrome b561 isoform (CYBDOM) were directly recorded (Picco et al 2015). This type of electron transport was mediated by a single transmembrane cytochrome; it was stimulated by cytosolic ascorbate and required an artificial electron acceptor like ferricyanide on the external face of the membrane.…”

Section: Discussionmentioning

confidence: 99%

“…The plasma membrane contains a complex redox system (for a recent review, see Lüthje et al 2013). Amongst other redox proteins, several types of cytochrome b have been identified, such as the Cytb561 family (Picco et al 2015), flavocytochromes b, and AIR12, an ascorbate-reducible cytochrome b. As mobile electron carriers, quinones are likely to be present in the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

Role of the NAD(P)H quinone oxidoreductase NQR and the cytochrome b AIR12 in controlling superoxide generation at the plasma membrane

Biniek

Heyno

Kruk

et al. 2016

Planta

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The quinone reductase NQR and the b-type cytochrome AIR12 of the plasma membrane are important for the control of reactive oxygen species in the apoplast. AIR12 and NQR are two proteins attached to the plant plasma membrane which may be important for generating and controlling levels of reactive oxygen species in the apoplast. AIR12 (Auxin Induced in Root culture) is a single gene of Arabidopsis that codes for a mono-heme cytochrome b. The NADPH quinone oxidoreductase NQR is a two-electron-transferring flavoenzyme that contributes to the generation of O in isolated plasma membranes. A. thaliana double knockout plants of both NQR and AIR12 generated more O and germinated faster than the single mutant affected in AIR12. To test whether NQR and AIR12 are able to interact functionally, recombinant purified proteins were added to plasma membranes isolated from soybean hypocotyls. In vitro NADH-dependent O production at the plasma membrane in the presence of NQR was reduced upon addition of AIR12. Electron donation from semi-reduced menadione to AIR12 was shown to take place. Biochemical analysis showed that purified plasma membrane from soybean hypocotyls or roots contained phylloquinone and menaquinone-4 as redox carriers. This is the first report on the occurrence of menaquinone-4 in eukaryotic photosynthetic organisms. We propose that NQR and AIR12 interact via the quinone, allowing an electron transfer from cytosolic NAD(P)H to apoplastic monodehydroascorbate and control thereby the level of reactive oxygen production and the redox state of the apoplast.

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“…In this study, the activity of NADH-ferricyanide oxidoreductase (NFORase) of PM vesicles from V.radiata hypocotyls was also measured. Ferricyanide (hexacyanoferrate (III), HCF III) was used as an electron acceptor and its reduced product was HCF II, which is convenient for the investigation of electron flow [16]. The reaction of HCF III + NADH → HCF II + NAD + was detected spectrophotometrically in this experiment.…”

Section: Other Enzymatic Characterization Of Hydrogenase Activity In Pmmentioning

confidence: 99%

Hydrogen evolution and absorption phenomena in plasma membrane of higher plants

Zhang

et al. 2020

Preprint

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Molecular hydrogen (H 2 ) exhibits a wide breadth of botanical effects especially in improving the resistance to abiotic stress. However, the primary target is not clear. Bidirectional hydrogenases have been well studied in microbes and alga, but there is a paucity of research in higher plants. Here, we used real-time spectrophotometry, gas chromatography and H 2 sensor to detect the H 2 -evolving and H 2 -uptake activity of the plasma membrane (PM) vesicles of higher plants. Net H + , Na + and K + fluxes were measured using Non-invasive Micro-test Technology (NMT). We found that the PM vesicles of Vigna radiata hypocotyls and Capsicum annuum stems have the ability of both oxidizing and producing H 2 . This two-way enzymatic activity coupled with the redox of ferricyanide/ferrocyanide and NADH/NAD + . H 2 affected the H + and Na + fluxes on the surface of V. radiata hypocotyls. These results demonstrate that the PM vesicles of higher plants have the abilities of H 2 evolution and absorption. The bidirectional activities may act as a valve to regulate the energy balance in plant cells.

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Direct Recording of Trans-Plasma Membrane Electron Currents Mediated by a Member of the Cytochrome b561 Family of Soybean

Cited by 22 publications

References 38 publications

Loss of a highly conserved sterile alpha motif domain gene ( WEEP ) results in pendulous branch growth in peach trees

Loss of a highly conserved sterile alpha motif domain gene ( WEEP ) results in pendulous branch growth in peach trees

Role of the NAD(P)H quinone oxidoreductase NQR and the cytochrome b AIR12 in controlling superoxide generation at the plasma membrane

Hydrogen evolution and absorption phenomena in plasma membrane of higher plants

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