Direct quantification of cancerous exosomes via surface plasmon resonance with dual gold nanoparticle-assisted signal amplification

Wang, Qing; Zou, Liyuan; Yang, Xiaohai; Liu, Xiaofeng; Nie, Wenyan; Zheng, Yan; Cheng, Quan

doi:10.1016/j.bios.2019.04.013

Cited by 160 publications

(96 citation statements)

References 46 publications

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“…These results verify the extreme inconsistency of exosome composition, even though the mother cells were of similar origin. Sina et al reported a real-time, label-free detection method for breast cancerrelated exosomes from complex biological samples using a SPR immunosensor [86]. Using this simple platform, HER2-specific exosomes were captured and detected on an anti-HER2-functionalized Au plate, at concentrations as low as 0.83 × 10 7 /mL.…”

Section: Analytical Methods For Extracellular Vesicles (Evs)mentioning

confidence: 99%

“…Each of the nanoislands can capture nine exosomes, meaning that the developed Au nanoisland platform can capture a much higher number of extracellular vesicles, thus offering a wide detection range from early stages to advanced stages of cancer. Wang et al described a 30 nm-sized dual Au nanoparticle-assisted SPR immunosensor for the sensitive detection of exosomes released from MCF-7 breast cancer cells [86] (Figure 7b). The aptamer/T 30 -linked Au nanoparticles were bound to the target exosomes and A 30 -coated Au nanoparticles could be captured on the aptamer/T 30 -linked Au nanoparticles through A-T hybridization.…”

Section: Analytical Methods For Extracellular Vesicles (Evs)mentioning

confidence: 99%

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Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Choi

Lee

Son

et al. 2020

Sensors

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For the early diagnosis of several diseases, various biomarkers have been discovered and utilized through the measurement of concentrations in body fluids such as blood, urine, and saliva. The most representative analytical method for biomarker detection is an immunosensor, which exploits the specific antigen-antibody immunoreaction. Among diverse analytical methods, surface plasmon resonance (SPR)-based immunosensors are emerging as a potential detection platform due to high sensitivity, selectivity, and intuitive features. Particularly, SPR-based immunosensors could detect biomarkers without labeling of a specific detection probe, as typical immunosensors such as enzyme-linked immunosorbent assay (ELISA) use enzymes like horseradish peroxidase (HRP). In this review, SPR-based immunosensors utilizing noble metals such as Au and Ag as SPR-inducing factors for the measurement of different types of protein biomarkers, including viruses, microbes, and extracellular vesicles (EV), are briefly introduced.

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Section: Analytical Methods For Extracellular Vesicles (Evs)mentioning

confidence: 99%

Section: Analytical Methods For Extracellular Vesicles (Evs)mentioning

confidence: 99%

Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Choi

Lee

Son

et al. 2020

Sensors

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“…This device is able to detect even 10 cancer cells/mL. Several authors employ the dual SPR assisted nanoparticle-mediated amplification: Park et al [71] to profile different transmembrane and intraexosomal proteins derived from ovarian cancer cells and Wang et al [72] to detect exosomes derived from MCF-7 breast cancer cells.…”

Section: Surface Plasmon Resonance (Spr)mentioning

confidence: 99%

Cancer Diagnosis through SERS and Other Related Techniques

Blanco-Formoso

Alvarez-Puebla

2020

IJMS

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Cancer heterogeneity increasingly requires ultrasensitive techniques that allow early diagnosis for personalized treatment. In addition, they should preferably be non-invasive tools that do not damage surrounding tissues or contribute to body toxicity. In this context, liquid biopsy of biological samples such as urine, blood, or saliva represents an ideal approximation of what is happening in real time in the affected tissues. Plasmonic nanoparticles are emerging as an alternative or complement to current diagnostic techniques, being able to detect and quantify novel biomarkers such as specific peptides and proteins, microRNA, circulating tumor DNA and cells, and exosomes. Here, we review the latest ideas focusing on the use of plasmonic nanoparticles in coded and label-free surface-enhanced Raman scattering (SERS) spectroscopy. Moreover, surface plasmon resonance (SPR) spectroscopy, colorimetric assays, dynamic light scattering (DLS) spectroscopy, mass spectrometry or total internal reflection fluorescence (TIRF) microscopy among others are briefly examined in order to highlight the potential and versatility of plasmonics.

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“…provided sufficient evidences that an electrochemical aptasensor based on Hemin/G‐Quadruplex‐Assisted Signal Amplification could detect exosomes derived from GC limit of 9.54 × 10 2 particles/μl (Table ), which was a visible and sensitive platform for the early diagnosis of GC. Wang et al . demonstrated another efficient surface plasmon resonance (SPR)‐reliant aptasensor for breast cancer, which allowed the detection of the limit of 5 × 10 3 particles/ml (Table ), as well as offering an avenue to capture breast cancer‐derived exosomes consequently.…”

Section: The Innovative Approaches To Detect Tumor‐derived Exosomes Fmentioning

confidence: 99%

“…Huang et al 89 provided sufficient evidences that an electrochemical aptasensor based on Hemin/ G-Quadruplex-Assisted Signal Amplification could detect exosomes derived from GC limit of 9.54 × 10 2 particles/μl (Table 2), which was a visible and sensitive platform for the early diagnosis of GC. Wang et al 90 demonstrated another efficient surface plasmon resonance (SPR)-reliant aptasensor for breast cancer, which allowed the detection of the limit of 5 × 10 3 particles/ml (Table 2), as well as offering an avenue to capture breast cancer-derived exosomes consequently. Liu et al 91 reported that a thermophoretic aptasensor based on enrichment of exosomes conjugated with aptamers could detect the tumor-derived EVs and seven kinds of EVs protein biomarkers from a serum sample (<1 μl), including breast cancer, lung cancer, ovarian cancer, prostatic cancer, HCC and lymphomas, which only cost one dollar within 3 hr ( Table 2).…”

Section: Aptasensor Techniquesmentioning

confidence: 99%

The cancer exosomes: Clinical implications, applications and challenges

Tang

Hou

et al. 2019

Intl Journal of Cancer

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The exosome is a small functional vesicle enriched in selected proteins, lipids and nucleic acids, displaying distinct molecular heterogeneity. Exosomes released can transform the extracellular matrix microenvironments, transmit signals and molecules to recipient cells and trigger changes in their pathophysiological functions. Tumor‐derived exosomes mediate the interactions of tumor cells and microenvironment significantly, and they stimulate tumor growth and development through specific signaling pathways related to metastasis, therapeutic resistance and immunosuppression. Exosome biogenesis from tumors often represents abundant biological information, and novel and efficient isolation and detection methods of exosomes provide a promising approach for tumor diagnosis and prognosis estimation. Moreover, exosome can even be developed as therapeutic agents for multiple disease models based on effective material transport characteristics and biofilm specificity. This review reports the clinical implications and challenges of exosomes in cancer progression, therapy resistance, metastasis and immune escape, and underlying cancerogenic pathological phenotypes including fibrosis and viral infection.

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Direct quantification of cancerous exosomes via surface plasmon resonance with dual gold nanoparticle-assisted signal amplification

Cited by 160 publications

References 46 publications

Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Cancer Diagnosis through SERS and Other Related Techniques

The cancer exosomes: Clinical implications, applications and challenges

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