Direct production of acetone–butanol–ethanol from waste starch by free and immobilized Clostridium acetobutylicum

Kheyrandish, Maryam; Asadollahi, Mohammad Ali; Jeihanipour, Azam; Doostmohammadi, Mohsen; Rismani-Yazdi, Hamid; Karimi, Keikhosro

doi:10.1016/j.fuel.2014.11.017

Cited by 68 publications

(35 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Butanol is recognized 56 as a candidate liquid biofuel substitute to gasoline due to its poten-57 tial properties as a fuel. Butanol is currently recognized as a neces-58 sary alternative and renewable biofuel due to the expected 59 exhaustion of petroleum oil and increased demand for energy, as 60 well as increasing global oil prices [1,2]. Butanol has many advan-61 tages as a biofuel.…”

mentioning

confidence: 99%

Biobutanol production by a new aerotolerant strain of Clostridium acetobutylicum YM1 under aerobic conditions

Al-Shorgani

Yusoff

Hamid

2015

Fuel

View full text Add to dashboard Cite

mentioning

confidence: 99%

Biobutanol production by a new aerotolerant strain of Clostridium acetobutylicum YM1 under aerobic conditions

Al-Shorgani

Yusoff

Hamid

2015

Fuel

View full text Add to dashboard Cite

“…In addition, ABE production was improved by increasing initial glucose concentration up to about 40 g/L (Long, Jones, & Woods, ). However, increasing the initial concentration of carbon source more than 75 g/L in the monoculture of C. acetobutylicum or C. beijerinckii (Ezeji, Qureshi, & Blaschek, ; Kheyrandish et al, ) decreases the yield of ABE. The concentration and yield of ABE were also affected by the initial concentration of yeast extract in the medium.…”

Section: Resultsmentioning

confidence: 99%

“…The preculture contained (g/L): Glucose, 50; peptone, 3; yeast extract, 1; and cysteine hydrochloride, 0.15 together with 1% (vol/vol) of P2 solution. P2‐stock solution (vitamins, minerals, and buffers) had the following composition (g/L): KH 2 PO 4 , 50; K 2 HPO 4 , 50; ammonium acetate, 220; para‐aminobenzoic acid, 0.1; thiamin, 0.1; biotin, 0.001; MgSO 4 ‐7H 2 O, 20; MnSO 4 ‐H 2 O, 1; FeSO 4 ‐7H 2 O, 1; NaCl, 1 (Kheyrandish et al, ; Lee et al, ; Salimi, ; Xue et al, ). After sterilization using an autoclave (at 111°C for 10 min), P2‐stock solution was added, and the pH was adjusted to 6.8 using 1 M NaOH.…”

Section: Methodsmentioning

confidence: 99%

Efficient butanol production under aerobic conditions by coculture of Clostridium acetobutylicum and Nesterenkonia sp. strain F

Ebrahimi

Amiri

Asadollahi

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

Clostridium acetobutylicum is widely used for the microbial production of butanol in a process known as acetone–butanol–ethanol (ABE) fermentation. However, this process suffers from several disadvantages including high oxygen sensitivity of the bacterium which makes the process complicated and necessitate oxygen elimination in the culture medium. Nesterenkonia sp. strain F has attracted interests as the only known non‐Clostridia microorganism with inherent capability of butanol production even in the presence of oxygen. This bacterium is not delimited by oxygen sensitivity, a challenge in butanol biosynthesis, but the butanol titer was far below Clostridia. In this study, Nesterenkonia sp. strain F was cocultivated with C. acetobutylicum to form a powerful “coculture” for butanol production thereby eliminating the need for oxygen removal before fermentation. The response surface method was used for obtaining optimal inoculation amount/time and media formulation. The highest yield, 0.31 g/g ABE (13.6 g/L butanol), was obtained by a coculture initiated with 1.5 mg/L Nesterenkonia sp. strain F and inoculated with 15 mg/L C. acetobutylicum after 1.5 hr in a medium containing 67 g/L glucose, 2.2 g/L yeast extract, 4 g/L peptone, and 1.4% (vol/vol) P2 solution. After butanol toxicity assessment, where Nesterenkonia sp. strain F showed no butanol toxicity, the coculture was implemented in a 2 L fermenter with continual aeration leading to 20 g/L ABE.

show abstract

“…onto a support or gel through adsorption, entrapment or covalent bond formation [75,76]. When Clostridium acetobutylicum bacteria were immobilized in LentiKats® capsules, a significant reduction in fermentation time from 27.8 h to 3.3 h (maintaining equal yields) compared with free-cell fermentation was observed [77].…”

Section: Batch Fermentationmentioning

confidence: 99%

Quest for sustainable bio-production and recovery of butanol as a promising solution to fossil fuel

Maiti

Gallastegui

Brar

et al. 2015

Int. J. Energy Res.

View full text Add to dashboard Cite

Summary Biobutanol has conventionally been generated by fermentation of carbohydrates derived from biomass (starch or sugar‐based feedstock, such as corn) using Clostridia strains (mainly C. beijerinckii and C. acetobutylicum) under anaerobic conditions in batch mode. Under these premises, it has been tough for the acetone–butanol–ethanol fermentation to compete with petro‐butanol production from an energy efficiency and material consumption standpoint. Challenges for butanol production from biomass comprised high cost of feedstock, scarcity of hyper‐butanol producing bacteria and low butanol yield, volumetric productivity and titre, leading to high water usage and separation‐purification costs. This article is an up‐to‐date review on several under explored sections, such as optimization of fermenter feed, microbial culture responsible for solvent production (co‐culture techniques and electro‐biochemical process), latest recovery techniques and the studies integrating in situ continuous fermentation processes. Biobutanol refinery way forward should build upon the use of low‐cost lignocellulosic matter and zero cost organic wastes and by‐products from food, agriculture, forestry, fermentation and paper industries as feedstock; optimized fermentation of such diversified feed with appropriate hyper‐butanol producing strains in biofilm reactors and integration of fermentation step with hybrid high butanol‐selective recovery techniques. Copyright © 2015 John Wiley & Sons, Ltd.

show abstract

Direct production of acetone–butanol–ethanol from waste starch by free and immobilized Clostridium acetobutylicum

Cited by 68 publications

References 27 publications

Biobutanol production by a new aerotolerant strain of Clostridium acetobutylicum YM1 under aerobic conditions

Biobutanol production by a new aerotolerant strain of Clostridium acetobutylicum YM1 under aerobic conditions

Efficient butanol production under aerobic conditions by coculture of Clostridium acetobutylicum and Nesterenkonia sp. strain F

Quest for sustainable bio-production and recovery of butanol as a promising solution to fossil fuel

Contact Info

Product

Resources

About