Direct polymerase chain reaction amplification of formalin-fixed, paraffin-wax-embedded tissue after automated sequential laser microdissection and pressure catapulting: Table 1

Abstract

A robust method to facilitate rapid laser microdissection and pressure catapulting (LMPC) coupled with direct polymerase chain reaction (dPCR) to eliminate the need for extraction of DNA before a PCR-based assay is described. This sequential LMPC–dPCR method is rapid and decreases the number of processing steps, reducing the chance of tissue loss and contamination.

Histological microdissection in diagnostic and investigative pathology

“…In h a false-colour image of the dissected tissue fragment has been digitally replaced in the space it occupied before removal, showing precision possible with mechanical microdissection. O'Kane et al 27 found extraction of DNA prior to PCR analysis was usually unnecessary with amplicons in the 107e232 bp range. The quantity of tissue needed for 90% successful amplification of 160 bp fragments by the methodology of Cawkwell and Quirke 28 was approximately 100 cells.…”