DIRECT PLANT REGENERATION FROM Pysalis peruviana L. EXPLANTS

Yaroshko, Olha

doi:10.15407/biotech12.02.056

Cited by 1 publication

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous work, we obtained regenerants for P. peruviana on the media MS30+ 1 mg/l kinetin (Kin) + 3 mg/l BAP and MS30 + 2 mg/l Kin + 1 mg/l BAP (33.33 % of regeneration on both) (Yaroshko, Kuchuk, 2019). In the current work, we got higher percentages of regeneration of the same species on the media MS30 + 1mg/l Zea and MS30 + 2mg/l Zea (38.46 % and 45 %).…”

Section: Resultsmentioning

confidence: 99%

“…The data were analyzed using the general procedure in the Software Package STATISTICA Version 12. Spearman's test and standard error were used for statistical processing of the obtained data; the procedure was described in detail in our previous work (Yaroshko, Kuchuk, 2019). In this work, the effect of different concentrations of growth regulator zeatin was compared with the appearance of different numbers of regenerants per one explant.…”

Section: Data Collection and Statistical Analysismentioning

confidence: 99%

“…A group of researchers headed by Otroshy received regenerants of P. peruviana on the MS30 medium + 4 mg/l BAP; MS30 + 1 mg/l Kin + 3 mg/l BAP from leaf explants and on the MS30 medium +2 mg/l Kin + 2 mg/l BAP; MS30 + 4 mg/l BAP + 1 mg/l Kin + 0.5 mg/l indolylbutyric acid (IBA) from nodular explants (Otroshy et al, 2013). Yaroshko and Kuchuk obtained regenerants of Physalis peruviana (Yaroshko, Kuchuk, 2019). Several scientific groups worked with Physalis minima (Afroz et al, 2009;Gupta, 1986;Sheeba et al, 2015;Mungole et al, 2011;Patel et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of the plant growth stimulant zeatin on regeneration capacity of some Physalis species in vitro culture

2020

The Journal of v. N. Karazin Kharkiv National University, Series "Biology"

View full text Add to dashboard Cite

The aim of the study was to find an efficient culture medium for regeneration of Physalis species in vitro to provide their further propagation ex vitro and obtain fructiferous plants from the regenerants. Physalis peruviana L., P. ixocarpa Broth. (cv. Likhtaryk), and P. pubescens L. (cv. Zarynka) were taken as plant material for the research. Plant introduction into culture and regenerant production were carried out in vitro; the rooting of mature plants and obtaining plants with ripe fruits took place in a greenhouse and in open ground (ex vitro). To obtain regenerants, we used Murashige and Skoog (MC30) medium supplemented with the growth stimulant zeatin (Zea) at a concentration of 0.5–3 mg/l. The growth stimulant 6-benzylaminopurine (BAP) was used to elongate the regenerant stalks, and the growth stimulator α-naphthylacetic acid (NAA) was used to initiate root formation. Plant regeneration frequency and the number of regenerants per explant served as indicators of the efficiency of various zeatin concentrations on the physalis regenerative capacity. The most effective media for the shoot regeneration from cotyledonous leaf explants were MC30 + 1 mg/l Zea and MC30 + 2 mg/l Zea. Regeneration frequency on these media was 46.15 % and 53.84 % for P. ixocarpa (cv. Likhtaryk), 38.46 % and 45 % for P. peruviana, and 27 % and 34 % for P. pubescens (cv. Zarynka) respectively. The emerged regenerants were separated from explants and transferred to MC30 medium supplemented with 1 mg/l of BAP + 0.1 mg/l of NAA for stalk growth and rooting. After a month of cultivation, juvenile plants were obtained. They were transferred to a greenhouse for adaptation, and later to open ground at the experimental plot. Three months after the regenerant emergence, we obtained fertile plants, which bloomed and bore fruit. The regenerants for domestic varieties of P. ixocarpa (cv. Likhtaryk) and P. pubescens (cv. Zarynka) were obtained for the first time. We established a direct relationship between the concentration of zeatin and both the frequency of plant regeneration and the number of regenerants per explant.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Data Collection and Statistical Analysismentioning

confidence: 99%