Direct Plant Regeneration From Mature Nodal Explants of Andrographis Echioides (L.) Nees – A Valuable Medicinal Plant

Abstract

The present study describes an effective, reproducible and rapid regeneration protocol for in vitro shoot development of the important medicinal plant Andrographis echioides (L.) Nees. via mature nodal explants inoculation on Murashige and Skoog (MS) medium supplemented with 6-Benzyl amino purine (BAP) 2.0 mg/l combined with α-Naphtalene acetic acid (NAA) 0.5 mg/l on which the highest percentage of shoot bud induction (90±2.88), number of shoot per explant (5±1.00) having a shoot length (2.0±0.57 cm) were produced. This investigation strongly recommends that the perfection of BAP (2.0 mg/l), NAA (0.5 mg/l) in combination with Gibberellic acid (GA 3 ) 0.5 mg/l on which a maximum percentage of shoot bud response (95±5.00), number of shoots per explants (9±1.00) and shoot length (6.8±1.11 cm) were found in A. echioides. MS medium, fortified with Indole-3-acetic acid (IAA) 1.0 mg/l combined with Indole-3-butyric acid (IBA) 0.5 mg/l on which highest percentage of response (90±5.77), number of roots per explants (12±1.15) and root length (6.5±1.26 cm) was observed. The regenerated plantlets were successfully transferred to field conditions with 80% survival rate.