Direct plant regeneration from cultured young leaf segments of sugarcane

Gill, R. S.; Malhotra, Pawan Kumar; Gosal, S. S.

doi:10.1007/s11240-005-9015-9

Cited by 45 publications

(29 citation statements)

References 11 publications

(7 reference statements)

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“…This result can be explained with the presence of predetermined cells which first dedifferentiate themselves, subsequently form new meristematic centres, and finally differentiate again producing new organs [34][35][36][37][38][39]. This process, due to cell totipotency, has been observed in several species [30,[40][41][42][43][44][45][46][47] and is very efficient for regeneration. Our results showed that AFLP is a very sensitive and reliable molecular marker technique for revealing specific genomic alterations induced by tissue culture and for identifying slightly different genotypes.…”

Section: Discussionmentioning

confidence: 99%

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Perrini

Alba

Ruta

et al. 2009

Cellular and Molecular Biology Letters

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A protocol for the induction of regeneration from leaves of Helichrysum italicum was established. Calli were found to form on the basal medium only when it was supplemented with thidiazuron (TDZ) alone or in combination with naphthalene acetic acid (NAA), with a percentage ranking of at least 80%. The hormone-free medium showed the highest percentage of shoot regeneration (62%) even though no callus formed. AFLP markers were employed to verify tissue culture-induced variation in the regenerated plantlets obtained by direct shoot regeneration or the indirect shoot regeneration process (callus formation). Seven out of the eleven AFLP primer pairs yielded polymorphic patterns. The average number of fragments per primer pair was 64.1. Singletons were represented by 12 (2.7%) fragments. Student’s T-test was performed both on the average number of shared fragments and on the nucleotide diversity, and no significant statistical difference was observed between the two regeneration treatments.

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Section: Discussionmentioning

confidence: 99%

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Perrini

Alba

Ruta

et al. 2009

Cellular and Molecular Biology Letters

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“…O mesmo ocorreu para o clone RB986419. A manutenção da alta umidade relativa, desde a retirada dos explantes do meio de cultivo até a retomada de seu crescimento, é fator de extrema importância para a sua sobrevivência (GRATTAPAGLIA; MACHADO, 1990).…”

Section: Fonte: Mudry (2011)unclassified

Embriogênese somática da cultivar RB966928 e do clone RB986419 de cana-de-açúcar (Saccharum spp.)

et al. 2013

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ResumoA cana-de-açúcar é uma cultura muito plantada devido a sua grande importância econômica, provenientes de seus produtos, etanol e açúcar. O Brasil é o maior produtor e exportador mundial de açúcar, sendo assim a cultura está freqüentemente inserida em programas de melhoramento genético. A transformação genética é estratégia para obtenção de cultivares resistentes e produtivas. Para obtenção de plantas transgênicas são necessários protocolos definidos e otimizados de regeneração in vitro. O objetivo desse trabalho foi avaliar a influência de diferentes concentrações de 2,4-D (ácido 2,4 diclorofenoxiacético) para obtenção de calos embriogênicos na cultivar RB966928 e no clone RB986419. Folhas imaturas, de plantas com 10 meses de idade, foram utilizadas como fonte de explantes. Cilindros foliares de 8 x 150 mm, segmentados em 4 mm, foram inoculados em meio de cultura MS com cinco concentrações de 2,4-D (1, 3, 9, 16 e 27 µM).Um segundo experimento foi realizado com as concentrações de 3, 9 e 16 µM de 2,4-D. Para os dois experimentos, a desdiferenciação do material ocorreu no escuro. Os calos foram transferidos após 45 dias de subcultivo para novo meio de cultura, com as mesmas concentrações de origem para sua multiplicação. A cultivar RB966928 teve respostas de embriogênese somática de 30 e 41% utilizando 16 µM de 2,4-D para o primeiro e segundo experimentos, respectivamente. O clone RB986419 apresentou, respectivamente, 26 e 80% de formação de calos embriogênicos, utilizando-se 9 µM de 2,4-D no primeiro e segundo experimento. Os calos formados foram transferidos para meio MS sem regulador de crescimento vegetal. As plântulas formadas foram aclimatizadas e colocadas em casa de vegetação. As melhores concentrações de 2,4-D para a formação de calos embriogênicos foram 16 µM para a cv. RB966928 e 9 µM para o clone RB986419. Palavras-chave: Calos embriogênicos, 2,4-D, cultivo in vitro AbstractThe sugar cane is a crop widely planted because of the economic importance of its products, sugar and ethanol. Brazil is the largest producer and exporter of sugar, so the crop is often inserted in breeding programs. Genetic transformation strategy is used to obtain resistant and productive cultivars. Defined and optimized in vitro regeneration protocols are required to obtain transgenic plants. The aim of this study was to evaluate the influence of different concentrations of 2,4-D (2,4-dichlorophenoxyacetic acid) to obtain embryogenic callus on cultivars RB966928 and RB986419 clone. Immature leaves from

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“…Lack of suitable multiplication procedures, for the mass multiplication of the elite germplasm, results in varietal decline (Kaur and Kapoor, 2016). The yield potential of sugarcane varieties is deteriorating, due to susceptibility to diseases, insects, admixture and changing edaphic and climatic environment (Gill et al, 2006). Moreover, the lack of rapid multiplication procedures has long been a serious problem in sugarcane breeding programs, as it takes 10-15 years of work to complete a selection cycle.…”

Section: Introductionmentioning

confidence: 99%

In Vitro direct plant regeneration using shoot tip explants in sugarcane (Saccharum officinarum L.) for rapid mass cloning

Kaur

Kapoor

2017

ASD

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Protocol for mass propagation, through direct plant regeneration in three commercial cultivars of sugarcane (CoJ64, CoJ83 & CoJ86) was standardized. Cultures were established from the young leaf segments (1.0 -1.5 cm), excised from spindle leaf (Shoot tip), used as explant source, were cultured on different media compositions based on Murashige and Skoog salts. Cultured explants exhibited swelling followed by direct shoot regeneration on media containing NAA, in all the three varieties. The highest frequency of shoot regeneration (87.58%) occurred on MS medium supplemented with NAA (5.0 mg/L) and Kinetin (0.5 mg/L) in variety, CoJ83. Medium devoid of NAA and supplemented with only kinetin did not induce direct shoot regeneration in any of the varieties thus tried. Subsequently profuse rooting of shoots was observed on the same medium and complete plantlets were recovered within 6 weeks. The sugarcane plantlets were acclimatized in greenhouse. The plantlets were hardened and transferred to soil, which exhibited good survival ranging from 85-90%. Tissue culture derived field-grown plants were normal and exhibited faster growth and better tillers. This protocol is a single step method without callus interphase for direct plant regeneration, which can be used commercially for rapid mass cloning of elite germplasm of sugarcane.

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Direct plant regeneration from cultured young leaf segments of sugarcane

Cited by 45 publications

References 11 publications

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

An evaluation of a new approach to the regeneration of Helichrysum italicum (Roth) G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Embriogênese somática da cultivar RB966928 e do clone RB986419 de cana-de-açúcar (Saccharum spp.)

In Vitro direct plant regeneration using shoot tip explants in sugarcane (Saccharum officinarum L.) for rapid mass cloning

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