Direct Organogenesis Protocol for in vitro Propagation of Chenopodium quinoa

Abstract

The direct organogenesis protocol of quinoa cultivar "Faro" was established in this study，respectively via axillary bud proliferation model and adventious bud formation model,using two different section ways from seedling cotyledons as explants.The most explant types for axillary bud proliferation model were cotyledons with petiole(approx. 1cm length), while for adventious bud formation were cotyledons without petiole. The medium ( 2MS supplemented with 3.0mg/L 6-BA ) was most suitable for axillary bud primary induction (97.5%), and the highest multiplication rate was attained in the subculture medium( 2MS supplemented with 3.0mg/L 6-BA and 0.1mg/L NAA) . we obtained 94.36% rejuvenation plantlets( >1cm in heighth) in the medium (2MS supplemented with 0.1mg/L 6-BA) . The highest adventitious buds formation was induced in the medium 2MS supplemented with 1.0mg/L 6-BA and 0.1mg/L NAA (12.14%). The most suitable medium for subculture proliferation was 2MS supplemented with 0.5mg/L 6-BA( 3.6 adventitous buds per explant). Rejuvenation plantlets (93.04% , >1cm in heighth) were successfully obtained in the medium (2MS supplemented with 0.3mg/L 6-BA). The most percentage of shoot rooting in vitro (51.04%) was abttained in the medium MS supplemented with 1.0mg/L IBA.The cultivar micropropagation protocol can be used for further genetic research of quinoa and prevention of viral diseases.