Direct Optical Visualization of DNA–DNA Interaction by Nanoparticle-Capture on Resonant PET-Films

Bauer, Maria; Haglmüller, Jakob; Pittner, Fritz; Schalkhammer, Thomas G. M.

doi:10.1166/jnn.2006.628

Cited by 8 publications

(9 citation statements)

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“…The methodology allows for applying minute amounts of an almost colorless substance on top of the corrugated surface to create a drastic and clearly visible color change. [53,54] The fact that very thin layers of these substances are sufficient makes the principle especially promising for any application that relies on diffusion of, e.g., a gas into the layer to produce absorption due to minimal diffusion times. [55,56] Applications could include fast gas sensors and PH detectors.…”

Section: Discussionmentioning

confidence: 99%

Remarkable Color Gamut Enhancement of Dye Lacquers Using Corrugated Surfaces

Mader

Martin

2021

Advanced Photonics Research

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Producing colors with colorless metals such as silver or aluminum is a fascinating application of plasmonic systems. [1][2][3][4][5][6] Such systems can be as simple as a single metal particle, where material, size, shape, and refractive index of the surrounding medium determine the color impression of the particle. [7][8][9][10][11] Indeed, all these parameters influence the absorption amplitude and the wavelength of the localized plasmon resonance within the particle. [12,13] On the other hand, one can also use a system supporting propagating surface plasmon-polaritons or in short surface plasmons. [14][15][16] These systems are often periodic at a length scale below the wavelength of visible light and have been optimized for color generation in transmission [17][18][19] as well as reflection, [20,21] where recently polarization-tunable colors were demonstrated. [22,23] In that case of periodic systems, the color is mainly determined by the material, periodicity, shape, and the refractive index of the surrounding medium. [24] The color generation of plasmonic systems is based on absorption due to the excitation of surface plasmons and not on an interference-based effect, where the periodicity determines the wavelengths which can excite surface plasmons. [14] In this article, we generate vivid colors by strongly enhanced absorption of dye lacquers applied conformally on square crossed metallic grating surfaces with identical periodicity in both dimensions. [25] Unlike previously published works, we focus here not only on the generation of color by the grating itself, [20,21] but explore its possible interactions with a dye lacquer to strongly enhance the dye absorption. Instead of the conventional approach that consists in embedding a plasmonic system in a nonabsorbing dielectric medium, [1,20] we apply a partially absorbing dye lacquer conformally and in direct contact to the grating surface.This idea is inspired by works of others, who measured a significant fluorescence emission enhancement for molecules placed at well-defined distances atop a metallic grating. [26,27] This concept was also used for light extraction to improve the efficiency of a light-emitting diode. [28] Instead of fluorescence emission, we focus here on the enhanced absorption of submicrometer thick (semi-) transparent dye lacquers applied conformally to metallic crossed gratings. Rather than varying the distance of the absorbing material to the metal surface, we vary the thickness and extinction coefficient κ of these lacquers. This detailed study reveals the mechanisms of enhancing absorption within the dye lacquer. The findings are not limited to color generation and may find applications in other fields, such as solar cell absorption improvement, [29] Raman spectroscopy, [30] or highly sensitive sensing applications. [31] 2. Results and Discussion 2.1. Measurement and Simulation of Gratings Self-Color

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Section: Discussionmentioning

confidence: 99%

Remarkable Color Gamut Enhancement of Dye Lacquers Using Corrugated Surfaces

Mader

Martin

2021

Advanced Photonics Research

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“…The cost of gold nanoparticle is cheaper than the urine strip test. In another study, Bauer et al aimed at the direct detection of sub-molecular layers of DNA with naked eye, based on the understanding of absorption property of metal nanoparticles [ 30 ]. The study focused on the nanolayer coated metallized-PET-chip setup and on the synthesis of DNA nanoparticle conjugates suitable for resonance amplifi ed absorption -point of care tests and the applied usage of those particles in the direct visualization of DNA-DNA binding events [ 30 ].…”

Section: Diagnostic Applications Of Nanoparticlesmentioning

confidence: 99%

Nanobiology in Medicine

Thangavel

2014

Nanomedicine

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“…In 1998, the application of the effect in a bioassay was reported [4]. Its potential as a novel (bio)analytical platform has been studied extensively since then (see [5][6][7]). In 2009, our group developed a novel microfluidic diagnostic device combining REA technology with an innovative fluidic setup operating by passive capillary force.…”

Section: Introductionmentioning

confidence: 99%

Immobilization Techniques and Integrated Signal Enhancement for POC Nanocolor Microfluidic Devices

Schlauf¹,

Assadollahi²,

Palkovits

et al. 2015

Journal of Nanomaterials

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Resonance enhanced absorption (REA) nanocolor microfluidic devices are new promising bioassay platforms, which employ nanoparticle-(NP-) protein conjugates for the immunodetection of medically relevant markers in biologic samples such as blood, urine, and saliva. The core component of a REA test device is a PET chip coated with aluminum and SiO 2 thin layers, onto which biorecognitive molecules are immobilized. Upon addition of a sample containing the analyte of interest, a NP-protein-analyte complex is formed in the test device that is captured on the REA chip, for example, via streptavidin-biotin interaction. Thereby, a colored symbol is generated, which allows optical readout. Silver enhancement of the bound nanoparticles may be used to increase the sensitivity of the assay. Herein, we demonstrate that adsorptive immobilization via a cationic polymeric interlayer is a competitive and fast technique for the binding of the capture protein streptavidin onto planar SiO 2 surfaces such as REA biochips. Moreover, we report the development of a silver enhancement technology that operates even in the presence of high chloride concentrations as may be encountered in biologic samples. The silver enhancement reagents may be integrated into the microfluidic assay platform to be released upon sample addition. Hereby, a highly sensitive one-step assay can be realized.

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Direct Optical Visualization of DNA–DNA Interaction by Nanoparticle-Capture on Resonant PET-Films

Cited by 8 publications

References 0 publications

Remarkable Color Gamut Enhancement of Dye Lacquers Using Corrugated Surfaces

Remarkable Color Gamut Enhancement of Dye Lacquers Using Corrugated Surfaces

Nanobiology in Medicine

Immobilization Techniques and Integrated Signal Enhancement for POC Nanocolor Microfluidic Devices

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