Direct observation of the elasticity-texture relationship in pyrolytic carbon via in situ micropillar compression and digital image correlation

Kabel, Joey; Edwards, Thomas Edward James; Sharma, Amit; Michler, Johann; Hosemann, Peter

doi:10.1016/j.carbon.2021.06.045

Cited by 13 publications

(6 citation statements)

References 60 publications

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“…However, accurately measuring localized tissue stiffness within deep, living three-dimensional (3D) tissues remains challenging 2 . Existing techniques primarily focus on subcellular measurements, are limited to in vitro cultures or dissected tissues and are unable to capture local variations in tissue stiffness 3 – 8 . Ultrasound-based shear-wave elastography offers non-invasive and quantitative assessment of in vivo tissue stiffness, but its resolution may be limited in detecting small tumours, resolving boundaries between tumours and surrounding tissue and assessing wave speed fluctuation in different tissue types 9 .…”

Section: Mainmentioning

confidence: 99%

Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo

Kim,

Min,

Kim

et al. 2023

Nat. Mater.

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Measuring cellular and tissue mechanics inside intact living organisms is essential for interrogating the roles of force in physiological and disease processes. Current agents for studying the mechanobiology of intact, living organisms are limited by poor light penetration and material stability. Magnetomotive ultrasound is an emerging modality for real-time in vivo imaging of tissue mechanics. Nonetheless, it has poor sensitivity and spatiotemporal resolution. Here we describe magneto-gas vesicles (MGVs), protein nanostructures based on gas vesicles and magnetic nanoparticles that produce differential ultrasound signals in response to varying mechanical properties of surrounding tissues. These hybrid nanomaterials significantly improve signal strength and detection sensitivity. Furthermore, MGVs enable non-invasive, long-term and quantitative measurements of mechanical properties within three-dimensional tissues and in vivo fibrosis models. Using MGVs as novel contrast agents, we demonstrate their potential for non-invasive imaging of tissue elasticity, offering insights into mechanobiology and its application to disease diagnosis and treatment.

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Section: Mainmentioning

confidence: 99%

Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo

Kim,

Min,

Kim

et al. 2023

Nat. Mater.

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“…Advanced techniques, such as X-ray computed tomography, enabled probing microstructure-property relationships [15][16][17] at the microscale. Transmission Electron Microscopy (TEM) is uniquely suitable to visualize submicron scale defects [10,[18][19][20], but it can be challenging to conduct in situ property measurements in the TEM [21]. TEM has enabled significant progress in atomic and nanoscale microstructural defects unique to layered materials.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Observation of Nanoscale Kink Band Mediated Plasticity in Ion-Irradiated Graphite: An In Situ TEM Study

Thomas,

Schoell,

Al-Mamun

et al. 2024

Materials

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Graphite IG-110 is a synthetic polycrystalline material used as a neutron moderator in reactors. Graphite is inherently brittle and is known to exhibit a further increase in brittleness due to radiation damage at room temperature. To understand the irradiation effects on pre-existing defects and their overall influence on external load, micropillar compression tests were performed using in situ nanoindentation in the Transmission Electron Microscopy (TEM) for both pristine and ion-irradiated samples. While pristine specimens showed brittle and subsequent catastrophic failure, the 2.8 MeV Au2+ ion (fluence of 4.378 × 1014 cm−2) irradiated specimens sustained extensive plasticity at room temperature without failure. In situ TEM characterization showed nucleation of nanoscale kink band structures at numerous sites, where the localized plasticity appeared to close the defects and cracks while allowing large average strain. We propose that compressive mechanical stress due to dimensional change during ion irradiation transforms buckled basal layers in graphite into kink bands. The externally applied load during the micropillar tests proliferates the nucleation and motion of kink bands to accommodate the large plastic strain. The inherent non-uniformity of graphite microstructure promotes such strain localization, making kink bands the predominant mechanism behind unprecedented toughness in an otherwise brittle material.

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“…Moreover, measurements at this length scale are different from tissue-level mechanical properties. Macroscopic characterization methods such as traction force microscopy, rheometry, and elastic micropillars have been used to measure forces at supracellular length scale [9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo

Kim

Min

Kim

et al. 2022

Preprint

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Measuring cellular and tissue mechanics inside intact living organisms is essential for interrogating the roles of force in physiological and disease processes, and is a major goal in the field of mechanobiology. However, existing biosensors for 3D tissue mechanics, primarily based on fluorescent emissions and deformable materials, are limited for in vivo measurement due to the limited light penetration and poor material stability inside intact, living organisms. While magneto-motive ultrasound (MMUS), which uses superparamagnetic nanoparticles as imaging contrast agents, has emerged as a promising modality for real-time in vivo imaging of tissue mechanics, it has poor sensitivity and spatiotemporal resolution. To overcome these limitations, we introduce magneto-gas vesicles (MGVs), a unique class of protein nanostructures based on gas vesicles and magnetic nanoparticles that produces differential ultrasound signals in response to varying mechanical properties of surrounding tissues. These hybrid protein nanostructures significantly improve signal strength and detection sensitivity. Furthermore, MGVs enable non-invasive, long-term, and quantitative measurement of mechanical properties within 3D tissues and organs in vivo. We demonstrated the performance of MGV-based mechano-sensors in vitro, in fibrosis models of organoids, and in vivo in mouse liver fibrosis models.

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Direct observation of the elasticity-texture relationship in pyrolytic carbon via in situ micropillar compression and digital image correlation

Cited by 13 publications

References 60 publications

Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo

Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo

Real-Time Observation of Nanoscale Kink Band Mediated Plasticity in Ion-Irradiated Graphite: An In Situ TEM Study

Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo

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