Direct Observation of Release of Cytochrome c from Lipid-Encapsulated Protein by Peroxide and Superoxide: A Possible Mechanism for Drug-Induced Apoptosis

Das, Neeladri; Gupta, Sayan; Mazumdar, Shyamalava

doi:10.1006/bbrc.2001.5403

Cited by 3 publications

(3 citation statements)

References 22 publications

(25 reference statements)

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“…Particularly sensitive to the applied therapy were primary melanoma cells (MEWO). In both cell lines the localization of Photofrin was observed mainly in mitochondrial membrane, which may lead to induction of intracellular disorders, release of apoptogenic proteins and finally to cell apoptosis [17]. Other researchers have also shown that the application of PDT in melanoma treatment is effective.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the influence of photodynamic reaction on the Me45 and MEWO cell lines in vitro

Choromańska¹,

Saczko²,

Kulbacka³

et al. 2012

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Aim of the studyPhotodynamic therapy (PDT) is an approved, minimally invasive and highly selective therapeutic approach to a variety of tumors. It is based on specific photosensitizer accumulation in the tumor tissue, followed by irradiation with visible light. The photochemical interactions of the photosensitizer, light and molecular oxygen produce singlet oxygen and other reactive oxygen forms. The imbalance between ROS generation and antioxidant capacity of the body gives rise to oxidative stress in the cell, which initiates cell death in PDT. The aim of this study was to investigate the effect of photodynamic reactions in human melanoma cell lines.Material and methodsPhotofrin® (Ph) was used for the photodynamic reaction in vitro as a photosensitizer. The primary cell line was MEWO cell line (granular fibroblasts), derived from a human melanoma. As a recurrent cell line we used Me45 cell line, derived from a lymph node metastasis of skin melanoma. We compared cell viability (MTT assay) to determine the effectiveness of applied therapy. The intracellular distribution of photosensitizer (Photofrin) and localization of mitochondria (Mito-Tracker Green) were detected by confocal microscopy.ResultsWe observed that Me45 and MEWO cell viability was dependent on the time of incubation after irradiation. In the recurrent cell line Ph accumulated mainly in the mitochondrial membranes and in MEWO cells also in the cytoplasm. The primary melanoma cell line exhibited significantly reduced cellular proliferation (below 50%) after photodynamic reaction with Ph.ConclusionsThe applied photodynamic reaction was more effective in primary melanoma cells. Additionally, mitochondrial localization of Ph can lead to disturbances of mitochondrial transmembrane potential and finally to release of apoptotic proteins.

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Section: Discussionmentioning

confidence: 99%

Comparison of the influence of photodynamic reaction on the Me45 and MEWO cell lines in vitro

Choromańska¹,

Saczko²,

Kulbacka³

et al. 2012

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“…necrosis, autophagic cell death). The death type depends on the properties and the concentration of the photosensitizer [4,10,11]. The type of cellular death is a very important subject in photodynamic applications.…”

Section: Introductionmentioning

confidence: 99%

Effects of electrophotodynamic therapy in vitro on human melanoma cells – melanotic (MeWo) and amelanotic (C32)

Choromańska¹,

Kulbacka²,

Rembiałkowska³

et al. 2015

Melanoma Research

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Photodynamic therapy has been considered ineffective for melanomas because of the competition between the absorbance of melanin from the melanoma and the absorbance of photosensitizers at the photosensitizer excitation light wavelength. Melanomas show considerable heterogeneity and resistance to phototherapy. The effectiveness of photodynamic therapy could be intensified by electroporation for enhanced transport of a photosensitizer by transient pores in the membrane. In this study, photodynamic therapy combined with electroporation was tested in vitro on the human melanoma cell lines melanotic melanoma (MeWo) and amelanotic melanoma (C32). Control experiments were conducted on human keratinocytes (HaCaT). Photofrin was used as a photosensitizer. Photosensitizer distribution, cloning efficacy test, comet assay, and assessment of apoptotic proteins were performed. Melanin levels were determined before and after photodynamic therapy. The experiments indicated that electroporation effectively supports the photodynamic method. It was found that photodynamic therapy with electroporation efficiently induces apoptosis in melanotic and amelanotic melanoma cells.

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“…While pathological significance for the peroxidatic activity of cytochrome c remains a topic of current research, there are several potential roles related to apoptotic death of cells [8][9][10][11] and to disruptions of the electron transport chain or mitochondrial membrane integrity [12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

A cytochrome c-enhanced peroxidation reaction with potential use in screening dietary antioxidants

Zhou

Davison

et al. 2004

Journal of Pharmaceutical and Biomedical Analysis

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Direct Observation of Release of Cytochrome c from Lipid-Encapsulated Protein by Peroxide and Superoxide: A Possible Mechanism for Drug-Induced Apoptosis

Cited by 3 publications

References 22 publications

Comparison of the influence of photodynamic reaction on the Me45 and MEWO cell lines in vitro

Comparison of the influence of photodynamic reaction on the Me45 and MEWO cell lines in vitro

Effects of electrophotodynamic therapy in vitro on human melanoma cells – melanotic (MeWo) and amelanotic (C32)

A cytochrome c-enhanced peroxidation reaction with potential use in screening dietary antioxidants

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