Direct observation of fluorescent proteins in gels: a rapid cost-efficient, and quantitative alternative to immunoblotting
Matthieu Sanial,
Ryan Miled,
Marine Alves
et al.
Abstract:The discovery of Green Fluorescent Protein (GFP) and its derivatives has revolutionized cell biology. These fluorescent proteins (FPs) have enabled the real-time observation of protein localization and dynamics within live cells. Applications of FP vary from monitoring gene/protein expression patterns, visualizing protein-protein interactions, measuring protein stability, assessing protein mobility and creating biosensors. The utility of FPs also extends to biochemical approaches through immunoblotting and pro… Show more
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