Direct observation of cytoskeleton-dependent trafficking of miRNA visualized by the introduction of pre-miRNA

Ishikawa, Toshinari; Sugawara, Ko; Zhang, Junwei; Funatsu, Takashi; Okabe, Kohki

doi:10.1016/j.isci.2024.108811

Cited by 2 publications

(2 citation statements)

References 54 publications

(88 reference statements)

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“…Meister et al, by studying Hela cells, demonstrated that mature miR-21 can be found in the cytoplasm and in the nucleus. This discovery suggested that a number of mature miRNAs may be transported back into the nucleus, where they may exert a functional activity [30,31]. In 2009, Földes-Papp et al used femtosecond laser microscopy to assess the association between molecular beacon probes and human single-stranded cellular targets of miR-122 [32].…”

Section: Evidence Of Mirnas In the Nucleusmentioning

confidence: 99%

Nuclear miRNAs: gene regulation activities

Billi,

De Marinis,

Gentile

et al. 2024

Preprint

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MicroRNAs are small non-coding RNAs, which contribute to the regulation of many physiolog-ical and pathological processes. Conventionally, miRNAs perform their activity in the cyto-plasm, where they regulate gene expression by interacting in a sequence-specific manner with mature messenger RNAs. Recent studies point to the presence of mature miRNAs in the nucleus. This review summarizes current findings regarding the molecular activities of nuclear miRNAs. These molecules can regulate gene expression at the transcriptional level by directly binding DNA on the promoter or the enhancer of regulated genes. MiRNAs recruit to these regions dif-ferent protein complexes, resulting both in activation or repression of transcription, through a number of molecular mechanisms. Haematopoiesis is presented as a paradigmatic biological process whereby nuclear miRNAs possess a relevant regulatory role. Nuclear miRNAs may af-fect gene expression also acting on nuclear mRNA processing and on the biogenesis of miRNA themselves by regulating pri-miRNA maturation. Overall, nuclear miRNAs are biologically ac-tive molecules that can be critical for the fine tuning of gene expression and deserve further studies in a number of physiological and pathological conditions

show abstract

Section: Evidence Of Mirnas In the Nucleusmentioning

confidence: 99%

Nuclear miRNAs: gene regulation activities

Billi,

De Marinis,

Gentile

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Meister et al, by studying Hela cells, demonstrated that mature miR-21 can be found in the cytoplasm and in the nucleus [ 36 ]. This discovery suggested that a number of mature miRNAs may be transported back into the nucleus, where they may exert a functional activity [ 36 , 37 ]. In 2009, Földes-Papp et al used femtosecond laser microscopy to assess the association between molecular beacon probes and human single-stranded cellular targets of miR-122 [ 38 ].…”

Section: Evidence Of Mirnas In the Nucleusmentioning

confidence: 99%

Nuclear miRNAs: Gene Regulation Activities

Billi,

De Marinis,

Gentile

et al. 2024

IJMS

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are small non-coding RNAs which contribute to the regulation of many physiological and pathological processes. Conventionally, miRNAs perform their activity in the cytoplasm where they regulate gene expression by interacting in a sequence-specific manner with mature messenger RNAs. Recent studies point to the presence of mature miRNAs in the nucleus. This review summarizes current findings regarding the molecular activities of nuclear miRNAs. These molecules can regulate gene expression at the transcriptional level by directly binding DNA on the promoter or the enhancer of regulated genes. miRNAs recruit different protein complexes to these regions, resulting in activation or repression of transcription, through a number of molecular mechanisms. Hematopoiesis is presented as a paradigmatic biological process whereby nuclear miRNAs possess a relevant regulatory role. Nuclear miRNAs can influence gene expression by affecting nuclear mRNA processing and by regulating pri-miRNA maturation, thus impacting the biogenesis of miRNAs themselves. Overall, nuclear miRNAs are biologically active molecules that can be critical for the fine tuning of gene expression and deserve further studies in a number of physiological and pathological conditions.

show abstract

Direct observation of cytoskeleton-dependent trafficking of miRNA visualized by the introduction of pre-miRNA

Cited by 2 publications

References 54 publications

Nuclear miRNAs: gene regulation activities

Nuclear miRNAs: gene regulation activities

Nuclear miRNAs: Gene Regulation Activities

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