Direct micromethod for diagnosis of acute and congenital Chagas' disease

Feilij, Hector; Müller, L; Cappa, S. M. González

doi:10.1128/jcm.18.2.327-330.1983

Cited by 159 publications

(83 citation statements)

References 9 publications

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“…However, the double examination is not always possible in rural conditions. Microhaematocrit concentration method is very easy to perform and reliable for the diagnosis of congenital Chagas disease diagnosis (Freilij et al 1983;. Other highly sensitive techniques, such as xenodiagnosis or haemoculture, are difficult to perform, expensive and require higher volumes of blood not suitable for newborns.…”

Section: Discussionmentioning

confidence: 99%

Risk factors and consequences of congenital Chagas disease in Yacuiba, south Bolivia

Salas

Cot

Schneider

et al. 2007

Tropical Med Int Health

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Summaryobjective To determine the risk factors of congenital Chagas disease and the consequences of the disease in newborns.methods Study of 2712 pregnant women and 2742 newborns in Yacuiba, south Bolivia. Chagas infection was determined serologically in mothers and parasitologically in newborns. Consequences of congenital Chagas disease were assessed clinically.results The prevalence of Chagas disease in pregnant women was 42.2%. Congenital transmission was estimated at 6% of infected mothers leading to an incidence rate of 2.6% among newborns. Main risk factors of congenital transmission were mothers' seropositivity and maternal Trypanosoma cruzi parasitaemia. Parity was higher in infected than in non-infected mothers, but it was not associated with the risk of congenital transmission. The rate of congenital infection was significantly higher in newborns from multiple pregnancies than in singletons. However, we did not observe statistically significant consequences of Chagas disease in newborns from single pregnancies or among twins.conclusions The main risk factors for congenital transmission were infection and parasitaemia of mothers. Consequences of the disease seemed mild in newborns from single pregnancies and perhaps more important in multiple births.keywords congenital Chagas disease, vertical transmission, pregnant women, newborns, Bolivia

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Section: Discussionmentioning

confidence: 99%

Risk factors and consequences of congenital Chagas disease in Yacuiba, south Bolivia

Salas

Cot

Schneider

et al. 2007

Tropical Med Int Health

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“…Approximately 2 ml of blood were taken from the heel for parasitological and serological studies. The blood samples were examined for T. cruzi by the microhaematocrit concentration technique using six 75 µl-capillary tubes per infant (Freilij et al 1983). Infants with a positive parasitological test were frequently not examined serologically to save labour, and they were immediately treated as described below.…”

Section: Diagnosis and Treatment Of Infantsmentioning

confidence: 99%

“…Early diagnosis of congenital T. cruzi infection is essential for treatment. Direct detection of T. cruzi in newborns is more effective by the microhaematocrit (or micro-Strout) concentration technique than by fresh blood examination (Freilij et al 1983). Histopathological examination of the placenta, xenodiagnosis and haemoculture are more costly and time-consuming than other direct methods, and the results only become available long after the infant and mother left the health facility.…”

Section: Introductionmentioning

confidence: 99%

Congenital transmission of Trypanosoma cruzi: an operational outline for detecting and treating infected infants in north‐western Argentina

Blanco¹,

Segura

Cura

et al. 2000

Tropical Med Int Health

119

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SummaryWe designed a set of procedures for first-line local health services to detect and treat the congenital transmission of Trypanosoma cruzi at a province-wide scale, and field-tested the programme in the province of Tucumán, northwestern Argentina, from 1992 to 1994. The programme consists of routine screening of pregnant women for seroreactivity to T. cruzi, serological and parasitological follow-up of the newborn at least twice during the first year of age, treatment of the infected infants, and evaluation of the outcome. 927 (5.5%) of 16 842 pregnant women were seroreactive to T. cruzi by indirect haemagglutination assay and ELISA. Twenty-one (6.7%) of 315 newborns to seroreactive mothers were diagnosed as infected with T. cruzi parasites microhaematocrit concentration before 30 days of age. Five newborns who initially tested negative had a T. cruzi infection detected by microhaematocrit and/or serological techniques at 3 or 6 months of age. Thus, congenital infection was diagnosed in 26 (7.1%) infants born to seroreactive women and residing in houses free of triatomine bugs. Four of 6 infants born to seroreactive mothers died during the first year of age and had some evidence of T. cruzi infection; one of the deaths was attributed to T. cruzi based on clinical evidence. After specific treatment with nifurtimox or benznidazole, 30 of 32 infants remained parasitologically and serologically negative. This study shows the feasibility of controlling the incidence of congenitally acquired T. cruzi infections at a province-wide scale by means of a specific screening programme at first-line health services level.

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“…Parasitological methods for detection of the acute phase have great sensitivity (direct methods) [21].…”

Section: Diagnosis Of Infectionmentioning

confidence: 99%

The Mouse Model as a Tool for Histological, Immunological and Parasitological Studies of Trypanosoma cruzi Infection

Villagrán-Herrera¹,

Martínez-Ibarra²,

Sánchez‐Moreno³

et al. 2018

Chagas Disease - Basic Investigations and Challenges

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The global expansion of Chagas disease is due to the constant migration of individuals from endemic countries with incidence of vector and nonvector transmission of Trypanosoma cruzi. The disease is present in its various stages: chronological characteristic signs and symptoms of the infection and its mechanism of immune system and cell and tissue damage. The first stage, which lasts 90 days approximately, is diagnosed by direct methods (blood smears stained with Giemsa, fresh and xenodiagnosis). The indeterminate-chronic stage is asymptomatic, but the growth and intracellular binary multiplication of the trypomastigotes continue promoting cell lysis and allowing parasites to infect other cells, with preferential tropism to organs producing mega syndromes such as cardiomyopathy, myocarditis, meningoencephalitis, megaesophagus and megacolon. Inadvertently, this process is repeated for several years leading to Chagas disease.The mouse inoculation allows checking the parasitemia in vivo and the development of the disease in short time (signs, behavior and tropism), histopathological alterations and detection of antibodies in serum. These parameters may vary when using different strains of T. cruzi from different geographical areas; Triatoma species due to their genetic variability are influenced by the environment, nutrition, reservoirs and habitat. The murine model ECA CD-1 has the ability to replicate human findings of Chagas disease.

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Direct micromethod for diagnosis of acute and congenital Chagas' disease

Cited by 159 publications

References 9 publications

Risk factors and consequences of congenital Chagas disease in Yacuiba, south Bolivia

Risk factors and consequences of congenital Chagas disease in Yacuiba, south Bolivia

Congenital transmission of Trypanosoma cruzi: an operational outline for detecting and treating infected infants in north‐western Argentina

The Mouse Model as a Tool for Histological, Immunological and Parasitological Studies of Trypanosoma cruzi Infection

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