Direct Measurement of Progesterone in Milk and Plasma by a Sensitive and Simple Enzymeimmunoassay

Wiel, D.F.M. van de; Koops, W.

doi:10.1016/s0007-1935(17)30993-4

Cited by 13 publications

(3 citation statements)

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“…Following the development of enzyme immunoassay (EIA) technology at the beginning of the 1980s for milk progesterone measurement (Arnstadt and Cleere 1981, Sauer and others 1981, van de Wiel and Koops 1982), on-farm milk progesterone tests quickly became commercially available (Nebel 1988). Consequently, progesterone measurements could for the first time be performed directly on farms, thus abrogating the requirement to transport milk samples to specialised laboratories.…”

Section: Introductionmentioning

confidence: 99%

“…Milk progesterone testing has a wide range of applications in animal production and veterinary requirements, including the identification of which cows are not pregnant 19–23 days post insemination (Burke and others 2012), confirmation of oestrous signs and pregnancy (Rajamahendran and others 1993, Romagnolo and Nebel 1993), as well as measuring the efficiency and accuracy of the detection of oestrus (Heersche and Nebel 1994), differentiation of ovarian cysts (Booth 1988 ; Sprecher and others 1988) and follow‐up endocrine therapy (Sprecher and others 1990), screening of embryo donors and embryo transfer recipients (Britt and Holt 1988, Herrler and others 1990) and assessment of ovulation detection performance (Morton and Wynn 2010). Following the development of enzyme immunoassay (EIA) technology at the beginning of the 1980s for milk progesterone measurement (Arnstadt and Cleere 1981, Sauer and others 1981, van de Wiel and Koops 1982), on‐farm milk progesterone tests quickly became commercially available (Nebel 1988). Consequently, progesterone measurements could for the first time be performed directly on farms, thus abrogating the requirement to transport milk samples to specialised laboratories.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of a lateral flow milk progesterone test with enzyme immunoassay as an aid for reproductive status determination in cows

Waldmann

Raud²

2016

Veterinary Record

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The lateral flow test (LFT) is an immunochromatographic method that utilises an immunostrip for non-laboratory diagnostic purposes. The present study evaluated a milk progesterone LFT against the enzyme immunoassay (EIA) to confirm oestrus and a non-pregnancy diagnosis. In total, 277 milk samples from 70 cows were analysed, collected on the day of artificial insemination and at 19 days, 21 days and 24 days post insemination. The level of accuracy of the LFT compared with the EIA was 95.0 per cent for milk samples containing <2 ng/ml progesterone and 97.0 per cent for milk samples containing >10 ng/ml progesterone. The validation of oestrus by the LFT was 98.6 per cent accurate using 2 ng/ml progesterone as the EIA estimate for oestrus. The test performance for a non-pregnancy diagnosis was subject to the day of milk sampling, showing the highest accuracy on day 24 post insemination for both tests. When optimised for maximum specificity, and compared with rectal palpation, the LFT had a sensitivity and specificity for non-pregnancy diagnosis on day 24 post insemination of 75.0 per cent and 100.0 per cent, respectively, with an overall accuracy of 84.4 per cent. The corresponding characteristics for the quantitative EIA were 85.0 per cent, 100.0 per cent and 90.6 per cent, respectively. The LFT results compared favourably with the quantitative milk progesterone EIA.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of a lateral flow milk progesterone test with enzyme immunoassay as an aid for reproductive status determination in cows

Waldmann

Raud²

2016

Veterinary Record

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“…The introduction of microtitration plates made the EIA a really practicable technique. Microtitration plate EIAs were already described for progesterone (Sauer et al 1982, van de Wiel and Koops 1982), estrone sulphate (Meyer and Gregor 1985), cortisol (Dhar et al 1986), 13,14-dihydro-15-keto-PGF 2a (Meyer 1986) and the boar taint compound 5a androst-16-en-3-one (Merckle et al 1986). The microtitration plate technique offers several advantages: the solid phase separation of bound and unbound tracer is very practical; the amount of reagents and waste is largely reduced; the tests are always clearly arranged and easy to survey; and the colorimetric evaluation of the results is finished within a few minutes.…”

Section: Introductionmentioning

confidence: 99%

Development of a sensitive microtitration plate enzyme‐immunoassay for the anabolic steroid trenbolone

Meyer

Hoffmann

1987

Food Additives and Contaminants

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The development of a competitive microtitration plate enzyme-immunoassay for monitoring trenbolone application to animals is described. 'Bridge heterology' was achieved with a rabbit antibody raised against 17 beta-trenbolone-hemisuccinate-BSA and 17 alpha-trenbolone glucuronide-alkaline phosphatase as a tracer. The required 17 alpha-trenbolone glucuronide was prepared by application of trenbolone acetate to a calf following isolation from urine by three HPLC steps. The glucuronide was linked to alkaline phosphatase by the mixed anhydride procedure. The EIA was performed by coating affinity purified sheep IgG antirabbit IgG to the microtitration plate well followed by the addition of sample, tracer and hormone-specific antibody. The absolute detection limit was less than 1 pg; 50% relative binding at approximately 3 pg which is about 20 times superior to our RIA. The sample blanks of purified extracts from urine, bile, faeces, liver and muscle were similar in both methods (EIA and RIA) and much lower than required for a reliable detection of positive samples. Assay variations were always less than 15%. For the EIA, radiolabelled trenbolone, which is not commercially available, is not necessary and according to our experience the EIA is more practicable and economic.

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Plasma progesterone concentrations in superovulated heifers determined by enzymeimmunoassay and radioimmunoassay

Boland

Foulkes²,

MacDonnell

et al. 1985

British Veterinary Journal

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Direct Measurement of Progesterone in Milk and Plasma by a Sensitive and Simple Enzymeimmunoassay

Cited by 13 publications

References 0 publications

Comparison of a lateral flow milk progesterone test with enzyme immunoassay as an aid for reproductive status determination in cows

Comparison of a lateral flow milk progesterone test with enzyme immunoassay as an aid for reproductive status determination in cows

Development of a sensitive microtitration plate enzyme‐immunoassay for the anabolic steroid trenbolone

Plasma progesterone concentrations in superovulated heifers determined by enzymeimmunoassay and radioimmunoassay

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